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GB 1886.180-2016: National Food Safety Standard -- Food Additives - β-cyclodextrin
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National Food Safety Standard -- Food Additives - β-cyclodextrin
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GB 1886.180-2016
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Basic data | Standard ID | GB 1886.180-2016 (GB1886.180-2016) | | Description (Translated English) | National Food Safety Standard -- Food Additives - ��-cyclodextrin | | Sector / Industry | National Standard | | Classification of Chinese Standard | X42 | | Word Count Estimation | 8,891 | | Date of Issue | 2016-08-31 | | Date of Implementation | 2017-01-01 | | Regulation (derived from) | Announcement of the State Administration of Public Health and Family Planning 2016 No.11 | | Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration | GB 1886.180-2016: National Food Safety Standard -- Food Additives - β-cyclodextrin---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Food additive - Cyclodextrin)
National Standards of People's Republic of China
National Food Safety Standard
Food additives β- cyclodextrin
Published 2016-08-31
2017-01-01 implementation
People's Republic of China
National Health and Family Planning Commission issued
National Food Safety Standard
Food additives β- cyclodextrin
1 Scope
This standard applies to the starch enzymatically purified preparation of food additives β- cyclodextrin.
Molecular formula and relative molecular mass 2
Formula 2.1
(C6H10O5) 7
2.2 relative molecular mass
1134.987 (according to 2011 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall comply with the requirements in Table 1.
Table 1 Sensory requirements
Project requires test methods
Color white or nearly white
Powder or a crystalline solid state
Slightly sweet taste
Proper amount of sample is placed in the white enamel plate, and its color was observed under natural light state,
And the taste of its products
3.2 Physical and Chemical Indicators
Physical and chemical indicators should be consistent with the provisions of Table 2.
Table 2. Physical and chemical indicators
Item Index Test Method
β- cyclodextrin content (dry basis), w /% 98.0 ~ 101.0 Appendix A A.3
Water, w /% ≤ 14 GB 5009.3 Karl Fischer method
Residue on ignition, w /% ≤ 0.1 GB/T 9741
Sugar, w /% ≤ 1.0 Appendix A A.4
Lead (Pb)/(mg/kg) ≤ 1.0 GB 5009.75 or GB 5009.12
Appendix A
Testing method
A.1 General Provisions
At time of the reagents in water and does not indicate other requirements, refer to three analytical reagent water and GB/T 6682 provisions. Test
When the standard solution, the standard solution, the determination of impurities, formulations and articles not indicate other requirements, are by GB/T 601, GB/T 602 and
GB/T 603 Preparation predetermined. The test does not indicate when the solution which is formulated with a solvent, refer to the aqueous solution.
A.2 Identification Test
A.2.1 Test Specific rotation
Weigh 1g sample was dissolved in 100mL of water, measured as GB/T 613 a predetermined, specific rotation should be 160 ° ~ 164 °.
A.2.2 chromatography test
Test, the peak retention times of the sample solution and the main peak of the chromatogram of the standard solution should be retained in the chromatogram β- cyclodextrin Determination
Consistent time.
Determination A.3 β- cyclodextrin content (dry basis) of
A.3.1 Reagents and materials
A.3.1.1 Methanol. HPLC grade.
A.3.1.2 water. a water GB/T 6682 provisions.
A.3.1.3 β- cyclodextrin standard. Purity ≥99%.
A.3.1.4 internal standard solution. 20mg/mL glycerol solution by 0.45μm filter membrane.
A.3.2 Instruments and Equipment
HPLC. equipped with a differential refractive index detector.
A.3.3 Reference chromatographic conditions
A.3.3.1 column. aminopropyl siloxane to bond the filler to 10μm porous silica gel column chromatography (φ4.6mm × 25cm), or
His equivalent column.
A.3.3.2 mobile phase. methanol-water = 946.
A.3.3.3 Column temperature. 30 ℃.
A.3.3.4 Detector temperature. 35 ℃.
A.3.3.5 flow rate. 1.5mL/min.
A.3.3.6 injection volume. 50μL.
A.3.4 Analysis step
Preparation of standard solution A.3.4.1
Weigh 1g standard β- cyclodextrin (accurate to 0.0001g) was placed in a 100mL volumetric flask, dissolve and dilute phase flows to the mark with
Degree, and mix. Take the above solution 1.0mL, 1.0mL internal standard solution was added. Chromatography using 0.45μm microporous membrane filter prior to analysis.
Preparation of sample solution A.3.4.2
1g sample was weighed (accurate to 0.0001g) was placed in a 100mL volumetric flask, and dissolved in mobile phase to volume, and mix. take
The solution 1.0mL, 1.0mL internal standard solution was added. Chromatography using 0.45μm microporous membrane filter prior to analysis.
A.3.5 Determination
In reference A.3.3 chromatographic conditions, respectively, the standard solution and sample solution was measured, and the peak area of the recording area of the internal standard peak. system
Repeating injection system suitability standard solution twice, the resultant area in response to the relative error is less than 2.0%.
A.3.6 calculation results
β- cyclodextrin content (dry basis) the mass fraction w1, calculated according to formula (A.1).
w1 =
Rucs × 100
Rscu × (100-w) ×
100% (A.1)
Where.
Ru --- peak ratio of the sample solution and the internal standard peak area;
--- CS standard solution concentration in grams per milliliter (g/mL);
100 --- mass conversion factor;
The ratio of the peak area of the internal standard peak and RS --- standard solution;
--- Cu concentration of the sample solution in grams per milliliter (g/mL);
W --- the water sample, in grams per hundred grams (g/100g).
The test result to the arithmetic mean of replicates results. Obtained in two independent determination results under repeatability conditions of absolute difference
Value is not greater than 0.5%.
A.4 Determination of reducing sugar
A.4.1 Reagents and materials
Alkaline copper citrate test solution A.4.1.1. Weigh 2.5g of copper sulfate, 5.0 g citric acid, 14.4 g of anhydrous sodium carbonate, dissolved in water and dilute
100mL.
A.4.1.2 acetic acid solution. 281.
A.4.1.3 iodine standard solution. c (
2I2
) = 0.05mol/L.
Hydrochloric acid solution A.4.1.4. 694.
A.4.1.5 sodium thiosulfate standard titration solution. c (Na2S2O3) = 0.05mol/L.
A.4.1.6 starch indicator solution. 10g/L.
A.4.1.7 analysis step
Weigh 1g sample was added 25mL of water, was slowly dissolved by heating, cooling, 20mL of test solution and an alkaline copper citrate few glass beads.
Heating the solution began to boil about 4min solution was maintained to boil 3min. After rapid cooling, the solution was added 100mL of acetic acid and 20.0mL
Standard iodine solution, the solution is shaken while the solution was added 25mL of hydrochloric acid. After the precipitate was dissolved, titrated with standard sodium thiosulfate solution titrated multiple
Iodine remaining. Near the end of the titration, 2mL starch indicator solution was added. Sodium thiosulfate standard titration solution should be greater than the volume required to titrate
Equal 12.8mL, i.e., a reducing sugar content of 1.0% or less.
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