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GB 1886.173-2016: Food additive -- Lactic acid
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Food additive -- Lactic acid
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GB 1886.173-2016
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Basic data | Standard ID | GB 1886.173-2016 (GB1886.173-2016) | | Description (Translated English) | Food additive -- Lactic acid | | Sector / Industry | National Standard | | Classification of Chinese Standard | X41 | | Word Count Estimation | 10,171 | | Date of Issue | 2016-08-31 | | Date of Implementation | 2017-01-01 | | Older Standard (superseded by this standard) | GB 2023-2003 | | Regulation (derived from) | Announcement of the State Administration of Public Health and Family Planning 2016 No.11 | | Issuing agency(ies) | National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration | GB 1886.173-2016: Food additive -- Lactic acid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Food additive - Lactic acid)
National Standards of People's Republic of China
National Food Safety Standard
Food Additives Lactic Acid
Issued on.2016-08-31
2017-01-01 implementation
People's Republic of China
National Health and Family Planning Commission released
Foreword
This standard replaces GB 2023-2003 "food additive lactic acid."
This standard compared with GB 2023-2003, the main changes are as follows.
--- Modify the sensory requirements;
--- Modify the content of lactic acid, L- lactic acid content of total cyanide indicator;
--- Remove the color, solubility ether, methanol (CH3OH in dollars), calcium, heavy metals (Pb) of the indicator;
--- Increased the lead index requirements and test methods;
--- Modify the identification test, lactic acid content, residue on ignition, citric acid, oxalic acid, phosphoric acid, tartaric acid testing methods;
--- Reference standard test methods section is adjusted to the latest released version.
National Food Safety Standard
Food Additives Lactic Acid
1 Scope
This standard applies to starch or sugar as raw material quality, the use of lactic acid bacteria or Rhizopus oryzae fermentation of prepared food additive lactic acid.
2 chemical name, structural formula, molecular formula and relative molecular mass
2.1 Chemical Name
α- hydroxy acid
2.2 formula
Formula 2.3
C3H6O3
2.4 relative molecular mass
90.08 (according to 2011 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall comply with the requirements of Table 1.
Table 1 Sensory requirements
Project requires test methods
Colorless to pale yellow color white to pale yellow
State clear liquid crystalline grain
Smell no odor or slight characteristic odor
Take the right amount of liquid sample and a solid sample is placed in a clean, dry
Beaker or porcelain plate, observe its color under natural light
Chak and status, and to smell the odor
3.2 Physical and Chemical Indicators
Physical and chemical indicators should be consistent with the provisions of Table 2.
Table 2. Physical and chemical indicators
Item Index Test Method
95.0 to 105.0 Appendix A lactate concentration, w /% of the value declared in A.3
L- lactic total lactic acid content, w /% ≥ 97 Appendix A A.4
Residue on ignition, w /% ≤ 0.1 A.5 in Appendix A
Chloride (Cl dollars), w /% ≤ 0.002 A.6 in Appendix A
Sulfate (SO4 dollars), w /% ≤ 0.005 Appendix A A.7
Iron salts (as Fe), w /% ≤ 0.001 Appendix A A.8
Cyanide/(mg/kg) ≤ 1 Appendix A A.9
Citric acid, oxalic acid, phosphoric acid, tartaric acid by Appendix A test A.10
By reducing sugar test A.11 in Appendix A
Easy carbon compound by test A.12 in Appendix A
Lead (Pb)/(mg/kg) ≤ 2.0 GB 5009.75 or GB 5009.12
Arsenic (As)/(mg/kg) ≤ 1.0 GB 5009.76
Appendix A
Testing method
A.1 General Provisions
At the time of the reagents and the water did not specify other requirements, refer to the three water analytical reagent and GB/T 6682 regulations. Test
When used in the standard solution, standard solution for measuring impurities, formulations and products not specified in other requirements, according to GB/T 601, GB/T 602,
GB/T 603 provisions prepared. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution.
A.2 Identification Test
A.2.1 Acid Test
10% aqueous sample of litmus paper acidic.
A.2.2 lactate test
The solid sample is dissolved in an appropriate amount of water or take a liquid sample, plus 10mL0.1mol L potassium permanganate solution/heating, which produce acetaldehyde
It smells.
A.3 Determination of lactic acid
A.3.1 Reagents and materials
A.3.1.1 sodium hydroxide solution. 40g/L.
A.3.1.2 sulfuric acid standard titration solution. c 12H2SO4
÷ = 0.5mol/L.
A.3.1.3 phenolphthalein indicator solution. 10g/L.
A.3.2 Analysis step
Weigh the sample 1g (accurate to 0.0002g), add 50mL water, adding sodium hydroxide solution accurately 20mL, boiled for 5min, add phenolphthalein
Indicator solution 2 drops hot with 0.5mol/L sulfuric acid standard titration solution titration, while for the blank test.
A.3.3 Calculation Results
Lactic acid content mass fraction w1, according to equation (A.1) Calculated.
w1 =
(V0-V1) × c × M
1000 × m ×
100% (A.1)
Where.
V0 --- volume of sulfuric acid standard titration solution consumed in the blank titration solution in milliliters (mL);
Volume V1 --- sulfate standard titration titration solution consumed by the sample solution, in milliliters (mL);
C --- the actual concentration of sulfuric acid standard titration solution, expressed in moles per liter (mol/L);
--- The M molar mass of lactic acid units of grams per mole (g/mol) [M (C3H6O3) = 90.08];
1000 --- quality conversion factor;
M --- the quality of the sample, in grams (g).
The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference
Not more than 0.2%.
Determination of total lactic acid A.4 L- lactic acid
A.4.1 Reagents and materials
GB/T 6682 provided a water.
A.4.2 Instruments and Equipment
High performance liquid chromatography.
A.4.3 reference chromatographic conditions
A.4.3.1 Column. with an optically active ligand exchange-type stationary phase coated or bonded to the silica filler column (φ4.6mm ×
15cm), or other equivalent column.
A.4.3.2 Mobile phase. 0.5g/L copper sulfate solution.
A.4.3.3 detection wavelength. 254nm.
A.4.3.4 Column temperature. 35 ℃.
A.4.3.5 flow rate. 0.5mL/min.
A.4.3.6 Injection volume. 20μL.
A.4.3.7 separation requirements. D-type and L-type separation ≥1.0.
A.4.4 Analysis step
Weigh the sample 0.05g (accurate to 0.0001g), add water or mobile phase and dilute to volume to 100mL, before chromatographic analysis using 0.45μm micro
Hole membrane filter. A.4.3 under chromatographic conditions were determined with reference, to give analysis sample peak area D- and L- lactic acid (D- milk
Acid retention time is about 10min, L- lactic acid retention time of about 12min), by area normalized and quantified.
A.4.5 Calculation Results
L- lactic acid, lactic acid levels of total mass fraction w2, according to equation (A.2) Calculated.
w2 =
AL
AL AD ×
100% (A.2)
Where.
AL --- sample L- lactic acid peak area;
AD --- sample D- lactic acid peak area.
The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference
Not more than 0.2%.
A.5 Determination of residue on ignition
A.5.1 Reagents and materials
sulfuric acid.
A.5.2 Instruments and Equipment
A.5.2.1 crucible.
A.5.2.2 high-temperature furnace.
A.5.2.3 dryer.
A.5.3 Analysis step
Take a sample of about 2g (accurate to 0.0001g), into the ignition to constant weight has been a crucible in the furnace slowly burn to completely carbonized, cold
To room temperature. Add about 0.5mL sulfuric moist, low-temperature heating to sulfuric acid vapor to escape completely. Then transferred to a high temperature furnace at 800 ℃
At 25 ℃ ± ignition to complete ash. Move inside the dryer and let cool to room temperature, accurately weighed, and then at a high temperature oven 800 ℃ ± 25 ℃ in burning
Till constant weight. Repeat twice before and after burning to weigh the difference does not exceed 0.5mg to constant weight.
A.5.4 Calculation Results
Burning residue mass fraction w3, according to equation (A.3) Calculated.
w3 =
m1-m0
m × 100%
(A.3)
Where.
The total mass m1 --- crucible and residue, in grams (g);
m0 --- crucible mass in grams (g);
M --- the quality of the sample, in grams (g).
The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference
Not more than 5% of the arithmetic mean.
A.6 chloride (Cl) Determination
A.6.1 Reagents and materials
A.6.1.1 nitric acid solution. 19.
A.6.1.2 silver nitrate solution. 17g/L.
A.6.1.3 chloride standard solution. 0.01mg/mL.
A.6.2 Analysis step
Weigh the sample 1g (accurate to 0.01g) placed in 50mL colorimetric tube, 10mL of nitric acid was added and the amount of water to a volume of about
40mL, add silver nitrate solution 1mL, diluted with water to 50mL, shake, in the dark place 5min. Sample tube with a standard tube turbidity,
Turbidity should not be deeper than the standard tube that sample mass fraction of chloride (Cl) is less than equal to 0.002%.
Preparation of standard tube. Imbibe 2mL chloride standard solution and the sample tube while the same treatment.
A.7 Sulfate (SO4 meter) measurement
A.7.1 Reagents and materials
A.7.1.1 hydrochloric acid solution. 13.
A.7.1.2 barium chloride solution. 250g/L.
A.7.1.3 sulfate standard solution. 0.1mg/mL.
A.7.2 Analysis step
Weigh the sample 2g (accurate to 0.01g) placed in 50mL colorimetric tube, add water, dissolve and dilute to 25mL, plus hydrochloric acid solution
1mL, placed in 30 ℃ ~ 35 ℃ water bath for 10min, plus barium chloride solution 3mL, shake, in the dark place 5min. Sample tube and
Standard pipe turbidity, turbidity should not be deeper than the standard tube that sample sulfate mass fraction (of SO4) less than or equal to 0.005%.
Preparation of standard tube. Imbibe 1mL sulfate standard solution and the sample tube while the same treatment.
A.8 iron salts (as Fe) Determination
A.8.1 Reagents and materials
A.8.1.1 ammonium persulfate.
A.8.1.2 hydrochloric acid solution. 13.
A.8.1.3 ammonium thiocyanate solution. 250g/L.
A.8.1.4 iron standard solution. 0.01mg/mL.
A.8.2 Analysis step
Weigh the sample 1g (accurate to 0.01g) placed in 50mL colorimetric tube, add water, dissolve and dilute to 25mL, plus hydrochloric acid solution 4mL
And ammonium persulfate 30mg, diluted with water to 35mL, plus 250g/L ammonium thiocyanate solution 3mL. Diluted with water to 50mL, shake, try
Like pipe and standard pipe than the color, the color should not be deeper than the standard tube that sample iron salts (as Fe) content of less than or equal
0.001%.
Preparation of standard tube. Imbibe 1mL iron standard solution and the sample tube while the same treatment.
A.9 Determination of cyanide
Warning --- potassium cyanide is highly toxic, this test need to be carried out carefully in a fume hood, to prevent skin contact, inhalation solution and steam.
A.9.1 Reagents and materials
A.9.1.1 standard potassium cyanide solution. Weigh accurately take potassium 25mg, set 100mL flask, dissolved in water and diluted to the mark, shake
uniform. When you use the precise amount of 5mL, set 250mL flask, diluted with water to the mark, shake, equivalent to 2μg per 1mL of CN-.
Solution should now use the existing.
A.9.1.2 trinitrophenol lithium solution. Weigh 0.25g of lithium carbonate and trinitrophenol 0.5g, add boiling water 80mL dissolved, cooling, plus
Water volume to 100mL.
A.9.2 Instruments and Equipment
A.9.2.1 Spectrophotometer.
A.9.2.2 sample processing apparatus (see Figure A.1).
Figure A.1 sample processing apparatus
A.9.3 Analysis step
A 2.0g sample taken home the bottle, add water, 5mL, shake immediately added 1mL trinitrophenol solution of lithium B cup into the A bottle
In stoppered, allowed to stand overnight in the dark. Remove the B cup, add water to accurately 2mL, mixing to obtain a sample solution. With a spectrophotometer at 500
Determination nm absorbance of the sample solution, and 1.0mL water was added a solution of potassium cyanide standard 5mL, supra operation resulting absorbance compared
It should not be greater that the cyanide content of the sample is less than equal to 1mg/kg.
A.10 Determination of citric acid, oxalic acid, phosphoric acid, tartaric acid
A.10.1 reagents and materials
Calcium hydroxide solution. Weigh calcium hydroxide 3g, add water sealed 1000mL, shake vigorously, place 1h. Pour supernatant with time.
A.10.2 Analysis steps
Weigh the sample 0.5g, add water, 5mL dissolved, mixing, adding calcium hydroxide solution 40mL, heated to boiling 2min, should not produce turbidity,
It is through testing.
A.11 Determination of reducing sugars
A.11.1 reagents and materials
A.11.1.1 sodium hydroxide solution.200g/L.
A.11.1.2 Fehling solution.
A.11.2 Analysis steps
Sample taken 0.1g, dissolved in 10mL water, neutralized with sodium hydroxide solution, Jiafei Lin 6mL solution, heated to boiling 2min, not
It should form a red precipitate, namely through tests.
Determination of carbon compounds A.12 Easy
A.12.1 reagents and materials
sulfuric acid.
A.12.2 Analysis steps
Take 5mL sulfuric acid solution, placed in a clean test tube, add sample 5mL attention along the wall, so that the liquid is divided into two layers, allowed to stand at 15 ℃
15min, then the color of the interface can not be deeper than a pale yellow, is by testing.
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