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GB 1886.169-2016 English PDF

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GB 1886.169-2016: [Including 2021XG1] Food additive -- Carrageenan
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Standard similar to GB 1886.169-2016

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Basic data

Standard ID GB 1886.169-2016 (GB1886.169-2016)
Description (Translated English) [Including 2021XG1] Food additive -- Carrageenan
Sector / Industry National Standard
Classification of Chinese Standard X41
Word Count Estimation 20,221
Date of Issue 2016-08-31
Date of Implementation 2017-01-01
Older Standard (superseded by this standard) GB 15044-2009
Regulation (derived from) Announcement of the State Administration of Public Health and Family Planning 2016 No.11
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB 1886.169-2016: [Including 2021XG1] Food additive -- Carrageenan

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(Food safety national standard - Food additive - Carrageenan) National Standards of People's Republic of China GB 1886.171-2016 National Food Safety Standard Food additives flavor nucleotides disodium 5' (Also known as flavor nucleotides disodium) Issued on. 2016-08-31 2017-01-01 implementation People's Republic of China National Health and Family Planning Commission released GB 1886.171-2016 National Food Safety Standard Food additives flavor nucleotides disodium 5' (Also known as flavor nucleotides disodium)

1 Scope

This standard applies to starch, sugar quality as raw material, fermentation or enzymatic method were 5'-inosinic acid disodium 5'-guanylate and disodium, then by Processing the resulting food additive flavor nucleotides 5'-phosphate (also known as flavor nucleotides disodium). 2 molecular formula, relative molecular mass and structural formula 2.1 disodium 5'-inosine monophosphate (IMP) 2.1.1 Formula C10H11N4Na2O8P · nH2O 2.1.2 Structural Formula 2.1.3 relative molecular mass (anhydrous) 392.17 (according to 2011 international relative atomic mass) 2.2 disodium 5'-guanylate (GMP) 2.2.1 Formula C10H12N5Na2O8P · nH2O GB 1886.171-2016 2.2.2 Structural Formula 2.2.3 relative molecular mass (anhydrous) 407.18 (according to 2011 international relative atomic mass)

3 Technical requirements

3.1 Sensory requirements Sensory requirements shall comply with the requirements of Table 1. Table 1 Sensory requirements Project requires test methods White or off-white color State crystal or crystalline powder Take the right amount of sample is placed in a clean, dry white porcelain dish, in natural light Under observe its color and status 3.2 Physical and Chemical Indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2. Physical and chemical indicators Item Index Test Method Flavor nucleotides disodium content (dry basis), w /% 97.0 ~ 102.0 Appendix A A.2 IMP mixing ratio/48.0% ~ 52.0 Appendix A A.2 GMP mixing ratio/48.0% ~ 52.0 Appendix A A.2 Loss on drying, w /% ≤ 25.0 GB 5009.3 a direct drying Transmittance (5% aqueous) /% ≥ 95.0 Appendix A A.3 pH (5% solution) 7.0 to 8.5 Appendix A A.4 Other nucleotides by test A.5 in Appendix A By amino acid test A.6 in Appendix A Appendix A test by ammonium A.7 Arsenic (As)/(mg/kg) ≤ 1.0 GB 5009.76 Heavy metals (Pb)/(mg/kg) ≤ 10 GB 5009.74 a drying temperature of 120 ℃, drying time of 4h. GB 1886.171-2016

Appendix A

Testing method A.1 General Provisions This standard reagents and water, did not indicate when the other requirements, refer to the three water analytical reagent and GB/T 6682 regulations. test Test used in the standard solution, determination of impurities standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T 602 and GB/T 603 provisions prepared. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution. A.2 flavor nucleotides disodium content (dry basis), measured IMP and GMP mixing ratio mixing ratio A.2.1 Reagents and materials Hydrochloric acid solution. 0.9mL amount of hydrochloric acid was slowly injected 1000mL water. A.2.2 Instruments and Equipment UV spectrophotometer (band width. 1nm, wavelength accuracy. ± 2.0nm, wavelength repeatability. ± 1.0nm, stray light. ≤ 0.02%). A.2.3 Analysis step A.2.3.1 Preparation of sample solution Weigh the sample 0.4g (accurate to 0.0001g) in 100mL beaker, dissolved with appropriate amount of water and dilute to 250mL, shake well and set aside. Draw the solution 5.0mL, diluted hydrochloric acid solution and dilute to 250mL, shake, as the sample solution spare. A.2.3.2 Determination The sample solution is injected into 1cm quartz cuvette, hydrochloric acid solution as the reference solution by UV spectrophotometer at 250nm, Absorbance was measured at 280nm. A.2.4 Calculation Results A.2.4.1 was calculated flavor nucleotides disodium content (dry basis) of Flavor nucleotides disodium content (dry basis) of the mass fraction w1, according to equation (A.1) Calculated. w1 = (A-1.463 × B) × 254.55 (7.976 × BA) × 47.769 m × 5 × 100-w4 × 100% (A.1) Where. A --- sample solution was measured at 250nm absorbance; B --- sample solution measured at 280nm absorbance; Sample weight m --- sample in grams (g); 5 --- volume conversion factor; 100 --- conversion factor; W4 --- Loss on drying of the sample,%; 1.463,254.55,7.976,47.769 --- dual wavelength absorbance was measured according to Beer's law, formula derivation process produces GB 1886.171-2016 Coefficients. The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference Not more than 1% of the arithmetic mean. Results are accurate to one decimal place. Calculation A.2.4.2 IMP and GMP mixing ratio mixing ratio IMP mass fraction w2, according to equation (A.2) Calculated. w2 = A-1.463 × B () × 342.18 m × 5 × 100% (A.2) GMP mass fraction w3, according to equation (A.3) Calculated. w3 = 7.976 × BA () × 62.55 m × 5 × 100% (A.3) Formula (A.2), formula (A.3) in. A --- sample solution was measured at 250nm absorbance; B --- sample solution measured at 280nm absorbance; Sample weight m --- sample in grams (g); 5 --- volume conversion factor; 1.463,342.18,7.976,62.55 --- dual wavelength absorbance measurement at, according to Beer's law, formula derivation process produces coefficient. It is mixing ratio X1, according to equation (A.4) Calculated. X1 = w2 w2 w3 × 100% (A.4) GMP mixing ratio X2, according to equation (A.5) Calculated. X2 = w3 w2 w3 × 100% (A.5) Formula (A.4), formula (A.5) in. w2 --- sample IMP mass fraction,%; w3 --- sample GMP mass fraction,%; The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference Not more than 1% of the arithmetic mean. Results are accurate to one decimal place. A.3 transmittance (5% aqueous solution) was measured A.3.1 Instruments and Equipment Spectrophotometer. A.3.2 Analysis step Weigh the sample 1g (accurate to 0.0001g), was dissolved in 20mL of water and shake. The above sample solution was placed 1cm cuvette in order Water as a blank using a spectrophotometer at 430nm measured transmittance of the sample solution and record the readings. The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference Value is not more than 0.5% of the arithmetic mean. A.4 pH (5% aqueous solution) was measured GB 1886.171-2016 A.4.1 Instruments and Equipment PH meter. A.4.2 Analysis step Weigh the sample 1g (accurate to 0.0001g), was dissolved in 20mL water, with the pH of the sample was measured by the acidity of the solution. The results parallel arithmetic mean of the measurement results shall prevail. Twice under the same condition of independent determination results obtained absolute difference Not more than 0.2. Determination A.5 Other nucleotides A.5.1 Reagents and materials A.5.1.1 Expand solution. isopropyl alcohol saturated ammonium water = 79219. A.5.1.2 chromatography paper. A.5.2 Instruments and Equipment A.5.2.1 ultraviolet analyzer. A.5.2.2 micro syringe. A.5.3 Analysis step Weigh the sample 1.0g, dissolved in water and dilute to 100mL, using chromatography paper spotted 10.0μL, to expand the solution to expand to about 30cm, Dry (or drying) filter, observed at 254nm UV analyzer. A.5.4 results found If only presented IMP, GMP two spots, as determined by experiments. A.6 Determination of amino acids A.6.1 Reagents and materials Ninhydrin solution. Take ninhydrin 0.5g, plus ethanol dissolved and set the volume to 100mL. A.6.2 Instruments and Equipment Water bath. A.6.3 Analysis step A sample was weighed 0.1g, dissolved in water and dilute to 100mL, draw the sample solution 5mL Shake added ninhydrin solution 1mL, to boil Water bath 3min. A.6.4 results found Color is not the solution, as determined by experiments. GB 1886.171-2016 Determination A.7 salts A.7.1 Reagents and materials A.7.1.1 magnesia. A.7.1.2 red litmus paper. A.7.2 Analysis step Weigh 0.1g sample in a test tube, was added 50mg of magnesium oxide and 1mL water, water moist red litmus paper, which will be linked into a test tube , The tube was heated in boiling water bath for 5min. A.7.3 results found Litmus paper is not a color, as determined by experiments.

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