GB/T 30957-2014 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 30957-2014: Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Status: Valid
Basic dataStandard ID: GB/T 30957-2014 (GB/T30957-2014)Description (Translated English): Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 7,774 Date of Issue: 7/8/2014 Date of Implementation: 1/10/2015 Quoted Standard: GB/T 6682; GB/T 14699.1; GB/T 20195 Regulation (derived from): 2014 National Standards Bulletin No. 18 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the feed of ochratoxin A immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed, feed concentrate supplement Determination of ochratox GB/T 30957-2014: Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of ochratoxin A in feeds. High performance liquid chromatography with immunoaffinity column clean-up ICS 65.120 B46 National Standards of People's Republic of China Feed Determination of ochratoxin A in Immunoaffinity column clean - High Performance Liquid Chromatography Issued on. 2014-07-08 2015-01-10 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard by the Ministry of Agriculture Feed Quality Supervision and Testing Center (Nanchang), Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Institute The study [National Feed Quality Supervision and Inspection Center (Beijing)], the quality of agricultural Standards and Testing Technology Research Institute, Shanghai Academy of Agricultural Sciences is responsible Drafted. The main drafters of this standard. Rao Hui, Wen Hong, Zhou Hua Jiao, Fu Jinhua, Xu Tian put, Rao Zhenghua, Yinteng Gui, Yang Linfen, Xing Lei, Zhao Weina, Zhao Zhihui. Feed Determination of ochratoxin A in Immunoaffinity column clean - High Performance Liquid Chromatography1 ScopeThis standard specifies the feed of ochratoxin A immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed, feed concentrate supplement Determination of ochratoxin A in. The standard limit of quantitation was 5.0μg/kg.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation Principle 3 After the sample methanol - water extract, the extract was filtered, diluted filtrate containing ochratoxin A through specific antibody immunoaffinity Column chromatography, eluted with PBS buffer and washed with pure water, methanol eluate was measured by high performance liquid chromatography with fluorescence detector Given ochratoxin A content of toxins.4 ReagentsUnless otherwise specified, reagents were analytical reagent grade water comply with GB/T 6682 in two water. 4.1 Methanol. HPLC grade. 4.2 acetonitrile. HPLC grade. 4.3 sodium chloride. 4.4 dibasic sodium phosphate. 4.5 potassium dihydrogen phosphate. 4.6 potassium chloride. 4.7 hydrochloric acid. 4.8 sodium bicarbonate. 4.9 Tween -20 (Tween-20). 4.10 acetic acid. 4.11 washing buffer. 25g of sodium chloride (4.3), 5g of sodium bicarbonate (4.8) was dissolved in water, was added 0.1mL Tween-20 (4.9), water Diluted to 1L. 4.12 PBS buffer solution. Weigh 8.0g of sodium chloride (4.3), 1.2g disodium hydrogen phosphate (4.4), 0.2g potassium dihydrogen phosphate (4.5), 0.2g chloride Potassium (4.6) was dissolved in 900mL of water, adjusted with concentrated hydrochloric acid (4.7) pH value to 7.0, to volume with water to 1L. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 30957-2014_English be delivered?Answer: Upon your order, we will start to translate GB/T 30957-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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