GB/T 30955-2014 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 30955-2014: Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Status: Valid
Basic dataStandard ID: GB/T 30955-2014 (GB/T30955-2014)Description (Translated English): Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 7,724 Date of Issue: 7/8/2014 Date of Implementation: 1/10/2015 Quoted Standard: GB/T 6682; GB/T 14699.1; GB/T 20195 Regulation (derived from): 2014 National Standards Bulletin No. 18 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the feed aflatoxin immunoaffinity column clean - HPLC method for measuring. This standard applies to the Determination of aflatoxin in feed. GB/T 30955-2014: Determination of aflatoxin B1, B2, G1, G2 content in feeds -- High performance liquid chromatography with immunoaffinity column clean-up---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of aflatoxin B1, B2, G1, G2 content in feeds. High performance liquid chromatography with immunoaffinity column clean-up ICS 65.120 B46 National Standards of People's Republic of China Determination of aflatoxin in feed B1, B2, G1, G2 of Immunoaffinity column clean - High Performance Liquid Chromatography DeterminationofaflatoxinB1, B2, G1, G2contentinfeeds- Issued on. 2014-07-08 2015-01-10 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard was drafted. Feed Science Institute, Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Research Institute, Zhejiang University [State Home Feed Quality Supervision and Inspection Center (Beijing)], Beijing seized Technology Co., Ltd. Viacom. The main drafters of this standard. YU Dong, Rao Zhenghua, LI Wei-fen, Li Lan, Wang Xiong, fruit flag, Liang rights. Determination of aflatoxin in feed B1, B2, G1, G2 of Immunoaffinity column clean - High Performance Liquid Chromatography1 ScopeThis standard specifies the feed of aflatoxin B1, B2, G1, G2 immunoaffinity column clean - HPLC determination method. This standard applies to feed Determination of aflatoxin B1, B2, G1, G2's. The standard detection limits. aflatoxin B1 was 0.2μg/kg, aflatoxin B2 was 0.2μg/kg, aflatoxin G1 is 0.3μg/kg, aflatoxin G2 is 0.3μg/kg. The standard limit of quantitation. aflatoxin B1 was 1.0μg/kg, aflatoxin B2 was 1.0μg/kg, aflatoxin G1 is 1.0μg/kg, aflatoxin G2 of 1.0μg/kg.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation Principle 3 After the sample methanol - after water extraction, the extract was filtered, diluted filtrate containing aflatoxin through specific antibody immunoaffinity layer Analysis of column chromatography purification, separation by high performance liquid chromatography, fluorescence detection after column photochemical derivatization determination of aflatoxins B1, B2, G1, G2 of content.4 ReagentsUnless otherwise indicated, all analytical reagents; laboratory water in line with GB/T 6682 in two water regulations, standards and mobile solution A water phase in line with the provisions of water. 4.1 Methanol. chromatographically pure. 4.2 Benzene. chromatography. 4.3 acetonitrile. chromatography. 4.4 aqueous methanol (82). Take 80mL of methanol (4.1) add 20mL water, mix well. 4.5 methanol-water (4555). Take 45mL of methanol (4.1) add 55mL water, mix well. 4.6 benzene acetonitrile (982). Take 2mL acetonitrile (4.3) was added 98mL benzene (4.2), mix well. 4.7 aflatoxin standard stock solution. benzene acetonitrile (982) solution (4.6) were prepared 0.100mg/mL aflatoxin B1, B2, G1, G2 standard stock solution and stored at 4 ℃ spare, you can use for 1 year. 4.8 aflatoxin mixed standard working solution. accurate Pipette the appropriate amount of aflatoxin B1, B2, G1, G2 standard stock solution (4.7), ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 30955-2014_English be delivered?Answer: Upon your order, we will start to translate GB/T 30955-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 30955-2014_English with my colleagues?Answer: Yes. 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