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Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 17812-2025: Determination of vitamin E in feeds - High performance liquid chromatography Status: Valid GB/T 17812: Historical versions
Basic dataStandard ID: GB/T 17812-2025 (GB/T17812-2025)Description (Translated English): Determination of vitamin E in feeds - High performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 14,181 Date of Issue: 2025-01-24 Date of Implementation: 2025-08-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 17812-2025: Determination of vitamin E in feeds - High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.ICS 65.120 CCSB46 National Standard of the People's Republic of China Replace GB/T 17812-2008 Determination of Vitamin E in Feed by High Performance Liquid Chromatography Released on 2025-01-24 2025-08-01 Implementation State Administration for Market Regulation The National Standardization Administration issued ForewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 17812-2008 "Determination of vitamin E in feed by high performance liquid chromatography" and GB/T 17812- Compared with.2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application of the saponification extraction method and the direct extraction method has been expanded to include concentrate supplements and compound premixed feeds (see Chapter 1); b) The extractant is changed to petroleum ether (boiling range 30℃~60℃) (see Chapter 4, Chapter 3 of the.2008 edition); c) Added vitamin E (dl-α-tocopherol) standard series solution (see 4.2.16); d) The sample preparation requirements have been changed (see 4.4, 3.5 of the.2008 edition); e) Added solid phase extraction method (see 4.5.1.2.2); f) Deleted normal phase chromatography conditions (see Chapter 3 of the.2008 edition); g) The detection wavelength has been changed (see 4.5.2.1, 3.6.2.1.2 of the.2008 edition); h) Added liquid chromatography reference conditions II (see 4.5.2.2); i) Added qualitative method and multi-point calibration for quantitative method (see 4.5.2.4 and 4.5.2.5); j) The test data processing has been changed (see 4.6 and 5.6, 3.6.2.3 and 4.6.2.3 of the.2008 edition); k) Added vitamin E (dl-α-tocopheryl acetate) standard solution series (see 5.2.5); l) The extraction temperature has been changed (see 5.5.1, 4.6.1 of the.2008 edition); m) Added qualitative method and multi-point calibration to quantitative method (see 5.5.2.3 and 5.5.2.4). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Shandong Provincial Animal Products Quality and Safety Center (Shandong Provincial Animal and Poultry Slaughter Technology Center), Sichuan Well Testing Technology Co., Ltd. Co., Ltd., Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences, and DSM Vitamins (Shanghai) Co., Ltd. The main drafters of this document are. Zhang Wei, Zhang Fengping, Zhang Yun, Sun Yanjun, Xie Li, Yan Chuanqi, Bu Yanlin, Wang Yingying, Zhu Liangzhi, Fan Xia, Li Junling, Li Huirong, Zhao Xuefeng, Zhao Xiaoyang, Yu Zhegao, Wu Liguo, Zhang Chao, Wu Jinyu. The previous versions of this document and the documents it replaces are as follows. ---First published as GB/T 17812-1999 in.1999 and first revised in.2008; ---This is the second revision. Determination of Vitamin E in Feed by High Performance Liquid Chromatography1 ScopeThis document describes a method for the determination of vitamin E in feeds by HPLC. The saponification extraction method in this document is applicable to compound feed, concentrated feed, concentrate supplement, compound premix feed, vitamin premix feed Determination of vitamin E (dl-α-tocopherol) in feed; direct extraction method is applicable to vitamin E in compound premixed feed and vitamin premixed feed (dl-α-tocopheryl acetate) determination. The quantification limit of the saponification extraction method in this document is 1.1 IU/kg; the quantification limit of the direct extraction method is 20 IU/kg.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples3 Terms and definitionsThere are no terms or definitions that require definition in this document. 4.Method 1.Saponification Extraction 4.1 Principle The vitamin E in the sample is saponified with alkaline solution, purified by liquid-liquid extraction or solid phase extraction, and determined by high performance liquid chromatography using the external standard method. Quantitative. 4.2 Reagents or materials Unless otherwise specified, only analytical grade reagents were used. 4.2.1 Water. GB/T 6682, Grade 1. 4.2.2 Anhydrous ethanol. chromatographic grade. 4.2.3 Anhydrous ethanol. 4.2.4 Petroleum ether. boiling range 30℃~60℃. 4.2.5 Methanol. chromatographic grade. 4.2.6 Acetonitrile. chromatographic grade. 4.2.7 Formic acid. chromatographically pure. 4.2.8 L-Ascorbic acid. 4.2.9 Butylated hydroxytoluene (BHT). 4.2.10 Anhydrous sodium sulfate. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 17812-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 17812-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 17812-2025_English with my colleagues?Answer: Yes. 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