GB/T 30956-2014 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 30956-2014: Determination of deoxynivalenol in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Status: Valid
Basic dataStandard ID: GB/T 30956-2014 (GB/T30956-2014)Description (Translated English): Determination of deoxynivalenol in feeds -- High performance liquid chromatography with immunoaffinity column clean-up Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 7,713 Date of Issue: 7/8/2014 Date of Implementation: 1/10/2015 Quoted Standard: GB/T 6682; GB/T 14699.1; GB/T 20195 Regulation (derived from): 2014 National Standards Bulletin No. 18 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the feed deoxynivalenol bacteria enolase (DON) immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed, concentrate supplement feed deo GB/T 30956-2014: Determination of deoxynivalenol in feeds -- High performance liquid chromatography with immunoaffinity column clean-up---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of deoxynivalenol in feeds. High performance liquid chromatography with immunoaffinity column clean-up ICS 65.120 B46 National Standards of People's Republic of China Determination of deoxynivalenol in feed bacteria enolase Immunoaffinity column clean - High Performance Liquid Chromatography Issued on. 2014-07-08 2015-01-10 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard by the Ministry of Agriculture Feed Quality Supervision and Testing Center (Nanchang), Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Institute The study [National Feed Quality Supervision and Inspection Center (Beijing)], Shanghai Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Research Institute, Shanghai City Feed Quality Supervision and Inspection Station is responsible for drafting. Drafters of this standard. Wen Hong, Rao Hui, Zhou Hua Jiao, Fu Jinhua, Yinteng Gui, Xu Tian put, Raozheng Hua, Yang Linfen, Xing Lei, Zhao Weina, Zhao Zhihui. Determination of deoxynivalenol in feed bacteria enolase Immunoaffinity column clean - High Performance Liquid Chromatography1 ScopeThis standard specifies the feed deoxynivalenol bacteria enolase (DON) immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed, concentrate supplement feed deoxynivalenol bacteria enolase (DON) measurement. The standard quantitative limit of 0.1mg/kg.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation Principle 3 Sample of deoxynivalenol fungi alcohol extracted with water, the extract was immunoaffinity column purification, after concentrated, with a UV detector Or diode array detector HPLC determination, external standard.4 ReagentsUnless otherwise specified, the reagents were of analytical grade water to comply with two water GB/T 6682 regulations. 4.1 Methanol. HPLC grade. 4.2 acetonitrile. HPLC grade. 4.3 polyethylene glycol (polyethyleneglycol, M = 8000). 4.4 HPLC mobile phase. Measure 100mL acetonitrile (4.2) to 1000mL volumetric flask, add water volume to 1000mL. mixing Even through 0.45μm filter and set aside. 4.5 deoxynivalenol bacteria enolase (deoxynivalenol) Standard. Purity ≥98%. 4.6 deoxynivalenol fungi alcohol standard stock solution. accurately weighed amount of deoxynivalenol fungi alcohol standard with methanol (4.1) dubbed Concentration of 0.1mg/mL standard stock solution stored at -20 ℃ refrigerator can be used for 3 months. 4.7 deoxynivalenol fungi alcohol standard working solution. deoxynivalenol fungi alcohol standard solution (4.6) with the mobile phase (4.4) and diluting 0.1μg/mL, 0.5μg/mL, 1.0μg/mL, 5.0μg/mL, 10.0μg/mL concentrations of deoxynivalenol bacteria enolase working standard solution. Deoxynivalenol fungi alcohol standard working solution stored at 2 ℃ ~ 8 ℃ refrigerator can be used for 7 days. 5. 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