GB/T 21329-2007 English PDF

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GB/T 21329-2007: Method for the determination of gentamicin residues in animal original food -- Enzyme-linked immunosorbent assay
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21329-2007	209	Add to Cart	3 days	Method for the determination of gentamicin residues in animal original food -- Enzyme-linked immunosorbent assay	Valid

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Basic data

Standard ID: GB/T 21329-2007 (GB/T21329-2007)
Description (Translated English): Method for the determination of gentamicin residues in animal original food -- Enzyme-linked immunosorbent assay
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 67.050
Word Count Estimation: 8,840
Date of Issue: 2007-10-31
Date of Implementation: 2008-04-01
Quoted Standard: GB/T 6379
Regulation (derived from): China National Standard Approval Announcement2007 No.13 (Total No.113)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the meat, enzyme-linked immunoassay method gentamicin residues in milk and milk powder, viscera, aquatic products. This standard specifies the meat, offal, fish, enzyme-linked immunoassay milk and milk powder gentamicin residues. This standard method detection limit: meat and aquatic products as 10. 0ug/kg, viscera, milk and milk powder as 20. 0ug/kg.

GB/T 21329-2007: Method for the determination of gentamicin residues in animal original food -- Enzyme-linked immunosorbent assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for the determination of gentamicin residues in animal original food. Enzyme-linked immunosorbent assay ICS 67.050 C53 National Standards of People's Republic of China Residues in food of animal origin gentamicin Determination ELISA Posted 2007-10-31 2008-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters of this standard. Shi Weiliang, Zhang Xiaofeng, Fang Ying, Xie Wen, Zhu Zhenjiang. This standard is a national standard was first published. Residues in food of animal origin gentamicin Determination ELISA

1 Scope

This standard specifies the method of enzyme-linked immunosorbent assay of gentamicin residues in meat, offal, fish, milk and milk powder. This standard applies to meat, offal, fish, milk and milk powder Determination of residual gentamicin. The standard method detection limit. meat and aquatic products 10.0μg/kg; offal, milk and milk powder to 20.0μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379 (all parts) of measurement methods and results Accuracy (trueness and precision) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987) Principle 3 Microplate coated with sheep anti-rabbit IgG antibody was added specific antibody (rabbit anti-gentamicin antibody), an enzyme-labeled gentamicin, gentamicin After ADM standard or sample, and specific antibody coated sheep anti-rabbit IgG antibody, while the free enzyme-labeled gentamicin and gentamicin And specific antibody binding neomycin competitive. Remove unbound enzyme-labeled gentamicin and gentamicin by washing, and then adding the substrate Color, absorbance was measured using a microplate reader, the absorbance of the sample is worth gentamicin content.

4 Reagents and materials

Remove otherwise stated, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 gentamicin detection kit (composition see Appendix A). 4.2 TCA. 4.3 sodium chloride. 4.4 potassium chloride. 4.5 potassium dihydrogen phosphate. 4.6 dibasic sodium phosphate. 4.7 sodium dihydrogen phosphate. 4.8 Tween-80. 4.9 3% TCA. weigh 3g TCA (4.2), water volume to 100mL. 4.10 PBS buffer. 8.5g sodium chloride, 1.15g disodium hydrogen phosphate, 0.27g sodium dihydrogen phosphate, water volume to 1L. 4.11 SDB buffer. 1.15g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride, 30g sodium chloride, 0.5mL spit -80 Temperature, water volume to 1L. 4.12 hexane. 4.13 gentamicin standards of USP grade. Preparation of standard solution of gentamicin 4.14. sterile water as a solvent formulated as 50mg/mL stock solution, stored at -20 ℃ condition. ......

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