GB 22255-2014 PDF EnglishUS$70.00 · In stock · Download in 9 seconds
GB 22255-2014: National Food Safety Standard -- Determination of Sucralose in Foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Obsolete GB 22255: Historical versions
Similar standardsGB 22255-2014: National Food Safety Standard -- Determination of Sucralose in Foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB22255-2014GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Determination of sucralose in foods ISSUED ON: JANUARY 28, 2015 IMPLEMENTED ON: JULY 28, 2015 Issued by: National Health and Family Planning Commission Table of ContentsForeword ... 3 1 Scope ... 4 2 Principle ... 4 3 Reagents and materials ... 4 4 Instruments and equipment ... 5 5 Analytical procedures ... 6 6 Presentation of analysis results ... 9 7 Precision ... 9 8 Others ... 9 Appendix A LC elution procedure in complex cases (applicable to evaporative light scattering detector) ... 11 Appendix B HPLC of sucralose ... 12 National food safety standard - Determination of sucralose in foods1 ScopeThis standard specifies the method for the determination of sucralose in food. This standard applies to the determination of sucralose in food.2 PrincipleThe sucralose in the specimen is extracted by methanol aqueous solution, to remove protein and fat; then purified and enriched by solid phase extraction column; separated by high performance liquid chromatography and reversed phase C18 chromatographic column; detected by evaporative light scattering detector or differential detector; qualified according to the retention time and quantified according to peak area.3 Reagents and materialsNote: Unless otherwise specified, the reagents used in this method are of analytical grade; the water is the grade-1 water specified in GB/T 6682. 3.1 Reagents 3.1.1 Methanol (CH3OH). 3.1.2 Acetonitrile (CH3CN): Chromatographically pure. 3.1.3 n-Hexane (C6H14). 3.1.4 Zinc acetate [Zn(CH3COO)2·2H2O]. 3.1.5 Potassium ferrocyanide [K4Fe(CN)6·3H2O]. 3.1.6 Neutral alumina (100 mesh ~ 200 mesh). 3.2 Preparation of reagents 3.2.1 Zinc acetate solution (219 g/L): Weigh 21.9 g of zinc acetate. Add 3 mL of acetic acid. Add water to dissolve it to 100 mL. 3.2.2 Potassium ferrocyanide solution (106 g/L): Weigh 10.6 g of potassium ferrocyanide. Add water to dissolve to 100 mL. 3.2.3 Methanol aqueous solution (75 + 25): Measure 75 mL of methanol. Add 25 mL of water. Mix well. 3.2.4 Acetonitrile aqueous solution (11 + 89): Measure 11 mL of acetonitrile. Add 89 mL of water. Mix well. 3.3 Standards Sucralose standard (C12H19C13O8): CAS No. 56038-13-2, purity ≥ 99%. 3.4 Preparation of standard solution 3.4.1 Standard stock solution of sucralose (10.0 mg/mL): Weigh 0.25 g (accurate to 0.0001 g) of sucralose standard product, in a 25 mL volumetric flask. Use water to dilute to the mark. Mix well. The concentration of sucralose is 10.0 mg/mL. The stock solution is stored in a refrigerator at 4 °C. The shelf life is 6 months. 3.4.2 Standard intermediate solution of sucralose (1.00 mg/mL): Pipette 5.00 mL of sucralose standard stock solution, into a 50 mL volumetric flask. Use water to dilute it to the mark. Mix well. The concentration of sucralose is 1.00 mg/mL. It is stored in a refrigerator at 4 °C. The shelf life is 3 months. 3.4.3 Standard working solution of sucralose: Pipette 0.200 mL, 0.500 mL, 1.00 mL, 2.00 mL, 4.00 mL of sucralose intermediate solution, into a 10 mL volumetric flask. Use water to dilute to the mark. The concentration of sucralose working solution is 0.0200 mg/mL, 0.0500 mg/mL, 0.100 mg/mL, 0.200 mg/mL, 0.400 mg/mL, respectively. 3.5 Materials Solid-phase extraction cartridges (200 mg, type N-vinylpyrrolidone and divinylbenzene hydrophilic-lipophilic balanced packing) are activated, by 4 mL of methanol and 4 mL of water sequentially, before use.4 Instruments and equipment4.1 High performance liquid chromatography: Equipped with a differential detector or an evaporative light scattering detector. 4.2 Balance: Sensitivity is 0.1 mg and 1 mg. 4.3 Vortex mixer. 4.4 Centrifuge: speed ≥ 3000 r/min. Weigh 2 g of the mixed specimen (accurate to 0.001 g). Put it in a 50 mL centrifuge tube. Add 1.0 g of neutral alumina. Add 3 mL of water. Oscillate it on a vortex mixer for 3 min. Then add 15 mL of methanol. The following steps are same as from 5.1.1.1 "Continue to oscillate for 30 s. Ultrasonically extract for 20 min. Centrifuge at 3000 r/min for 10 min", to 5.1.1.3 "The filtrate is the prepared specimen solution, which is set aside". 5.1.3 Alcohol-containing specimens (fermented wine, blended wine) Weigh 5 g of the mixed specimen (accurate to 0.001 g). Place it in a 50 mL evaporating dish. Evaporate to dryness on a boiling water bath. Use 1.00 mL of acetonitrile aqueous solution (11 + 89), to dissolve the residue. Make the solution pass through a 0.45 μm filter membrane. The filtrate is the prepared specimen solution, which is set aside. 5.1.4 Beverages Weigh 5 g of the mixed specimen (accurate to 0.001 g). Place it in a 15 mL centrifuge tube. Add 5 mL of water. Oscillate on a vortex mixer for 30 s. Centrifuge at 3000 r/min for 10 min. The following steps are processed according to 5.1.1.3. 5.1.5 Flavored fermented milk and milk tea Weigh 1 g ~ 5 g of the mixed specimen (accurate to 0.001 g). Place it in a 50 mL centrifuge tube. Add 5 mL of water. Oscillate on a vortex mixer for 3 min. Then add 15 mL of methanol, 0.50 mL of zinc acetate solution, 0.50 mL of potassium ferrocyanide solution. The following steps are same as from 5.1.1.1 "Continue to oscillate for 30 s. Ultrasonically extract for 20 min. Centrifuge at 3000 r/min for 10 min", to 5.1.1.3 "The filtrate is the prepared specimen solution, which is set aside". The pretreatment of different specimens requires a specimen blank test at the same time. 5.2 Instrument reference conditions 5.2.1 Chromatographic column: C18 column (4.6 mm × 150 mm, 5 μm) or equivalent performance. 5.2.2 Mobile phase: water + acetonitrile = 89 + 11. Note: When the detection sample matrix is complex AND the strongly retained substances affect the subsequent detection, an elution procedure can be adopted (applicable to evaporative light scattering detectors), as shown in Appendix A. 5.2.3 Flow rate: 1.0 mL/min. 5.2.4 Column temperature: 35 °C. 5.2.5 Differential detector conditions: ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB 22255-2014 be delivered?Answer: The full copy PDF of English version of GB 22255-2014 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB 22255-2014_English with my colleagues?Answer: Yes. The purchased PDF of GB 22255-2014_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 22255-2014 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds.Question 5: Should I purchase the latest version GB 22255-2014?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 22255-2014 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.How to buy and download a true PDF of English version of GB 22255-2014?A step-by-step guide to download PDF of GB 22255-2014_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).Step 2: Search keyword "GB 22255-2014". Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart. Step 4: Select payment option (Via payment agents Stripe or PayPal). Step 5: Customize Tax Invoice -- Fill up your email etc. Step 6: Click "Checkout". Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively. Step 8: Optional -- Go to download PDF. Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice. See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9 |
