US$439.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 26401-2011: National food safety standards of food additives arachidonic acid grease (fermentation)
Status: Obsolete
| Standard ID | USD | BUY PDF | Lead-Days | Standard Title (Description) | Status |
| GB 26401-2011 | 439 |
Add to Cart
|
3 days
|
National food safety standards of food additives arachidonic acid grease (fermentation)
| Obsolete |
Basic data
Standard ID: GB 26401-2011 (GB26401-2011)
Description (Translated English): National food safety standards of food additives arachidonic acid grease (fermentation)
Sector / Industry: National Standard
Classification of Chinese Standard: X40
Classification of International Standard: 67.220.20
Word Count Estimation: 11,119
Date of Issue: 2011-03-15
Date of Implementation: 2011-05-15
Regulation (derived from): Ministry of Health Bulletin No. 7 of 2011
Issuing agency(ies): Ministry of Health of the People's Republic of China
Summary: This Chinese standard applies to the use of Mortierella (Mortierlla alpina) strains, obtained by fermentation of arachidonic acid (ARA) grease.
GB 26401-2011: National food safety standards of food additives arachidonic acid grease (fermentation)
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National food safety standards of food additives arachidonic acid grease (fermentation)
National Standards of People's Republic of China
People's Republic of China Ministry of Health issued
Issued on. 2011-03-15
2011-05-15 implementation
National Food Safety Standard
Food Additives arachidonic acid oil (fermentation)
National Food Safety Standards
Food Additives arachidonic acid oil (fermentation)
1 Scope
This standard applies to the use of Mortierella (Mortierlla alpina) strain, obtained by fermentation of arachidonic acid (ARA)
grease.
2 relative molecular mass and structural formula
2.1 Structure formula
2.2 relative molecular mass
304.5 (according to 2007 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements. comply with Table 1.
Table 1 Sensory requirements
Project requires test methods
Pale yellow color sample taken 10 mL, placed in a test tube, using natural light in the visual
The method to observe its color and morphology, using sniffing gas inspection
taste.
This product has a unique odor odor
Oily liquid state organization
3.2 Physical indicators. to comply with Table 2.
Table 2. Physical and chemical indicators
4 Other requirements
Item Index Test Method
Content (C20H32O2 triglycerides dollars), w /% ≥ 38.0 Appendix A A.3
Unsaponifiables, w /% ≤ 4.0 GB/T 5535.1 ether extraction
Water, w /% ≤ 0.1 GB 5009.3 vacuum drying method
Insoluble impurities, w /% ≤ 0.2 GB/T 15688
Solvent residue/(mg/Kg) ≤ 1.0 GB/T 5009.37
The acid value (in dollars KOH)/(mg/g) ≤ 1.0 GB/T 5009.37
Peroxide value/(meq/kg) ≤ 5.0 GB/T 5009.37 titration
Trans fatty acids, w /% ≤ 1.0 GB 5413.36
Aflatoxin B1/(μg/kg) ≤ 5.0 GB/T 5009.22
Total arsenic (As)/(mg/kg) ≤ 0.1 GB/T 5009.11 hydride generation atomic fluorescence spectrometry
Lead (Pb)/(mg/kg) ≤ 0.1 GB 5009.12 Graphite Furnace Atomic Absorption Spectrometry
2 products should be installed in a suitable container, protected from light vacuum or nitrogen stored optimum storage temperature of the product or less -5 ℃.
3 Appendix A
Testing method
A.1 Safety requirements
Reagents used in this test method is toxic and corrosive, must be careful when operating. Should immediately splashed on the skin, such as
Rinse water, severe cases should be treated immediately. When using flammable, do not use open flame heating.
A.2 General Provisions
Unless otherwise indicated, the reagents used were of analytical grade, third grade water testing water GB/T 6682-2008 stipulated. Inspection
The standard titration solution, impurity measurement standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601,
GB/T 602 and GB/T 603 provisions prepared.
A.3 arachidonic acid (ARA) in triglyceride Determination
A.3.1 Reagents and materials
A.3.1.1 arachidonic acid (ARA) triglyceride standard. Purity ≥99%.
A.3.1.2 tridecane acid triglyceride standard. Purity ≥99%.
A.3.1.3 potassium hydroxide.
A.3.1.4 anhydrous methanol.
A.3.1.5 n-heptane, chromatography.
A.3.1.6 petroleum ether boiling range, 30 ℃ ~ 60 ℃.
A.3.1.7 methanol solution of potassium hydroxide (4 mol/L). Weigh 22.4g of potassium hydroxide (A.3.1.3) in the beaker, adding an appropriate amount of anhydrous methanol
(A.3.1.4) dissolved and transferred to 100mL volumetric flask, when the methanol solution of potassium hydroxide was cooled to room temperature, dilute with anhydrous methanol
Release to the mark.
A.3.1.8 arachidonic acid (ARA) triglyceride stock solution (10 mg/mL). Weigh 250 mg (accurate to 0.000 1 g) of arachidonic
Acid (ARA) triglycerides (A.3.1.1) in 25 mL volumetric flask, add appropriate amount of n-heptane (A.3.1.5) to dissolve and dilute to the mark
Degree, shake.
A.3.1.9 tridecane acid triglyceride internal standard stock solution (10 mg/mL). Weigh 250 mg (accurate to 0.000 1 g) alkyl tridecane
Triglyceride (A.3.1.2) in 25 mL volumetric flask, add appropriate amount of n-heptane (A.3.1.5) to dissolve and dilute to the mark.
A.3.1.10 formulated standard working solution
Table A.1 Pipette respectively right amount of arachidonic acid (ARA) triglyceride stock solution (A.3.1.8) in six 10 mL volumetric flask, respectively
Transferred to 2.0 mL tridecane acid triglyceride internal standard stock solution (A.3.1.9), was added n-heptane (A.3.1.5) diluted to the mark.
Table A.1 standard working solution of arachidonic acid (ARA) triglyceride
Numbering
Volume arachidonic acid (ARA) in triglyceride stock solution
(ML)
Concentration of standard working solution of arachidonic acid (ARA) triglyceride
(Mg/mL)
2 0.1 0.1
3 0.5 0.5
4 1.0 1.0
5 2.0 2.0
6 3.0 3.0
4A.3.1.11 nitrogen purity ≥99.999%.
A.3.1.12 helium, purity ≥99.9999%.
A.3.2 Instruments and Equipment
A.3.2.1 GC - MS.
A.3.2.2 gas chromatograph with flame ionization detector.
A.3.2.3 analytical balance. a sense of the amount of 0.01 g, 0.1 mg.
A.3.2.4 centrifuge.
A.3.2.5 shaker.
A.3.3 qualitative analysis
A.3.3.1 arachidonic acid (ARA) oil
Weigh 0.25 g (accurate to 0.01 g) sample was 50 mL volumetric flask, add appropriate amount of n-heptane (A.3.1.5) to dissolve and diluted
To the mark. Accurately suck 2 mL of n-heptane was dissolved in 10 mL capped centrifuge tube, add 0.2 mL of methanol solution of potassium hydroxide
(A.3.1.7), shake vigorously for 1 min or more, place 10 min (methylation reaction). The organic layer was cloudy, so that it can be clarified by centrifugation.
Take the upper organic phase alternate solution.
A.3.3.2 Determination
A.3.3.2.1 GC conditions
a) Column. DB-5ms (30.0 m × 0.25 mm × 0.25 μm) capillary column, or equivalent person;
b) Column temperature program. 100 ℃ maintained 3 min, to 10 ℃/min was raised to 260 ℃, holding 8 min;
c) Carrier gas. Helium (A.3.1.12);
d) flow rate. 1.0 mL/min;
e) Inlet temperature. 250 ℃;
f) Injection mode. splitless (split ratio 1.10);
g) Injection volume. 1 μL.
A.3.3.2.2 MS conditions
a) Ion source. EI source, 70 eV;
b) interface temperature. 280 ℃;
c) Ion source temperature. 230 ℃;
d) quadrupole temperature. 150 ℃;
e) data acquisition modes. full-scan;
f) mass scan range. 20 m/z ~ 330 m/z;
g) Solvent delay 3.5min.
A.3.3.3 results found
Spectrum of the sample should be arachidonic acid (ARA) methyl ester standards spectra (Appendix B in Figure B.1) consistent.
A.3.4 Quantitative Analysis
A.3.4.1 Principle
Sample arachidonic acid (ARA) of potassium hydroxide in methanol, generating arachidonic acid (ARA), methyl hydrogen flame distribution
Ionization detector gas chromatography were determined within the standard method, the results of arachidonic acid (ARA) triglyceride count.
A.3.4.2 Preparation of test solution
Preparation A.3.4.2.1 arachidonic acid (ARA) oil sample solution
5 Weigh 0.04 g sample (accurate to 0.000 1 g) in 10 mL volumetric flask, add 2.0 mL tridecane acid triglyceride internal standard reservoir
Preparation of solution (A.3.1.9), the following press A.3.3.1 "adding an appropriate amount of n-heptane (A.3.1.5) take the upper organic phase solution standby" operation. each
Do at least two parallel parts of the test solution samples.
Preparation A.3.4.2.2 blank solution
Except that no sample addition, the method for processing according to A.3.4.2.1.
A.3.4.3 standard working solution esterified
Imbibe respectively in series of standard working solution A.3.1.10 2 mL to 10 mL centrifuge tube with lid, from the following press A.3.3.1 "added
0.2 mL of potassium hydroxide in methanol (A.3.1.7) take the upper organic phase solution standby "operation.
A.3.4.4 Determination
A.3.4.4.1 GC conditions
a) Column. DB-23 (30.0 m × 0.25 mm × 0.25 μm) capillary column, or equivalent person;
b) Column temperature program. 90 ℃ to maintain 5 min, 9 ℃/min raised to 240 ℃, holding 5 min, 3 ℃/min raised to 250 ℃;
c) Carrier gas. nitrogen (A.3.1.11);
d) flow rate. 1.33 mL/min;
e) Inlet temperature. 250 ℃;
f) Injection mode. splitless (split ratio of 1. 100);
g) Detector temperature. 300 ℃;
i) Injection volume. 1 μL.
Drawing A.3.4.4.2 standard curve
Determination of the conditions listed by A.3.4.4.1, the standard solution of methyl sequentially after injection, measuring arachidonic acid (ARA) and methyl
Thirteen of methyl carbon peak area. Arachidonic acid (ARA) methyl ester peak area of standard solution of arachidonic acid (ARA) Gan
Oil proportional to the concentration of tri-ester. Standard working solution of arachidonic acid (ARA) triglyceride concentrations (mg/mL) as the abscissa,
Arachidonic acid (ARA) with the 13th carbon of methyl ester peak area ratio of the vertical axis, the standard curve. Arachidonic acid (ARA)
Chromatogram of methyl methacrylate and tridecane Refer to Appendix C in Figure C.1.
According to formula (A.1) calculation of the regression parameters.
() Yaxb = × (A.1)
Where.
y-- arachidonic acid (ARA) with the 13th carbon of methyl ester peak area ratio;
a-- regression slope of the curve;
x-- standard working solution of arachidonic acid (ARA) triglyceride concentration, in milligrams per milliliter (mg/mL);
b-- intercept of the regression curve.
A.3.4.4.3 Determination of test solution
The blank solution (A.3.4.2.2) and the sample solution (A.3.4.2.1) followed by injection, deducting the blank value obtained arachidonic acid (ARA)
Methyl ester/methyl ester of tridecane peak area ratio.
A.3.4.5 Calculation Results
Sample arachidonic acid (ARA) triglyceride content X1 according to formula (A.2) Calculated.
by
100 ×
×× = (A.2)
6 Where.
X1-- sample arachidonic acid (ARA) triglyceride content,%;
After the test solution y-- esterified arachidonic acid (ARA) with the 13th carbon of methyl ester peak area ratio;
a-- regression slope of the curve;
b- intercept of the regression curve;
V-- the final volume of the test solution, in milliliters (mL);
m - mass of the sample, in milligrams (mg).
If the content of arachidonic acid (ARA) dollars, multiplied by the conversion factor of 0.9597.
Calculate the arithmetic mean of the parallel determination results representing the value to two decimal places.
A.3.4.6 Repeatability
Two independent determination results under the absolute difference in repeatability condition must not exceed 10% of the arithmetic mean.
7 Appendix B
Arachidonic acid (ARA) methyl ester standard gas chromatography - mass spectrum
NOTE. The spectra from NIST library
Figure B.1 arachidonic acid (ARA) methyl ester standard quality spectrum
8 Appendix C
After the mixed standard working solution of methyl gas chromatogram
1-- tridecane acid methyl ester (9.9min)
2-- arachidonic acid (ARA) methyl ester (18.8min)
Figure C.1 mixed standard working solution of methyl gas chromatogram
......
Tips & Frequently Asked Questions:
Question 1: How long will the true-PDF of GB 26401-2011_English be delivered?
Answer: Upon your order, we will start to translate GB 26401-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.
Question 2: Can I share the purchased PDF of GB 26401-2011_English with my colleagues?
Answer: Yes. The purchased PDF of GB 26401-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.
Question 3: Does the price include tax/VAT?
Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs):
List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?
Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
