GB 26405-2011 PDF English
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GB 26405-2011: National food safety standard - Food additives - Lutein
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GB 26405-2011: National food safety standard - Food additives - Lutein
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Status: Valid
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GB 26405-2011: National food safety standard - Food additives - Lutein
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB26405-2011GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard - Food Additive - Lutein ISSUED ON: MARCH 15, 2011 IMPLEMENTED ON: MAY 15, 2011 Issued by: Ministry of Health of the People's Republic of China
Table of Contents
1 Scope ... 3 2 Molecular formula, structural formula and relative molecular mass ... 3 3 Technical requirements ... 3 Appendix A Inspection methods ... 5 National Food Safety Standard - Food Additive - Lutein1 Scope
This Standard applies to the food additive lutein, which is made from Tagetes erecta L. oleoresin through saponification and extraction. Commercial lutein products can contain edible vegetable oils, dextrins, antioxidants and other auxiliary materials, which are used for standardization and other purposes.2 Molecular formula, structural formula and relative
molecular mass 2.1 Molecular formula C40H56O2 2.2 Structural formula 2.3 Relative molecular mass 568.88 (according to the international relative atomic mass in 2007)3 Technical requirements
3.1 Sensory requirements: shall meet the requirements of Table 1. 3.2 Physical and chemical indicators: shall meet the requirements of Table 2.Appendix A
Inspection methods A.1 General provisions The reagents and water that are used in this Standard, when no other requirements are specified, refer to analytical reagents and grade-III water which is specified in GB/T 6682-2008. The standard titration solution which are used in the test, the standard solutions for impurity determination, the preparations and products, are all prepared in accordance with the provisions of GB/T 601, GB/T 602, and GB/T 603, when no other requirements are specified. The solution used in the test, if not indicated which solvent is used, refers to aqueous solution. A.2 Identification test A.2.1 Insoluble in water, extremely-slightly soluble in n-hexane, soluble in ethanol and chloroform. A.2.2 In the test for determining the total carotenoid content, the sample solution has a maximum absorption near 446 nm. A.2.3 In the test for determining the lutein content, the retention time of the main peak of the sample solution in the liquid chromatogram shall be the same as the retention time of lutein in the standard solution. A.3 Determination of total carotenoids A.3.1 Reagents and materials A.3.1.1 Solvent: a mixture of n-hexane, acetone, toluene and absolute ethanol (10:7:7:6). A.3.1.2 Absolute ethanol. A.3.2 Instruments and apparatuses Ultraviolet-visible spectrophotometer. A.3.3 Analysis steps Accurately weigh 0.03 g of the sample, accurate to 0.000 1 g; use the solvent of A.3.1.1 to dissolve it; transfer to a 100 mL volumetric flask; add the solvent of A.3.1.1 to fix volume to the mark; shake well. Take 1 mL of this sample solution in a 100 mL volumetric flask; use absolute ethanol to fix volume to the A.4.3.1 Chromatographic column: silica gel column, 4.6 mm × 250 mm, particle size of 3 μm; or other equivalent chromatographic column. A.4.3.2 Mobile phase: prepare according to the volume ratio, that is, n-hexane: ethyl acetate = 70: 30; after mixing uniformly, use a 0.45 μm filter membrane for filtration; perform ultrasonic degassing for later use. A.4.3.3 Column temperature: room temperature. A.4.3.4 Mobile phase flow rate: 1.5 mL/min. A.4.3.5 Injection volume: 10 μL. A.4.4 Analysis steps A.4.4.1 Preparation of standard solution Respectively weigh about 0.01 g of lutein and zeaxanthin standard substances, accurate to 0.000 1 g; use mobile phase to dissolve; transfer to a 50 mL volumetric flask; add mobile phase to fix volume to the mark; shake well for later use. A.4.4.2 Preparation of sample solution Weigh 0.025 g ~ 0.027 g of the sample, accurate to 0.000 1 g; use mobile phase to dissolve it; transfer it into a 100 mL volumetric flask; add mobile phase to fix volume to the mark; shake well for later use. A.4.4.3 Determination Under the reference chromatographic conditions of A.4.3, determine the standard solution of lutein and zeaxanthin; record the chromatogram. It is required that the number of theoretical plates shall be at least 5 000 based on the lutein peak, and the separation between the main peak and the isomer peak shall be at least 1.5. Under the reference chromatographic conditions of A.4.3, measure the sample solution; determine the chemical composition based on the retention time of the standard; record the chromatogram. Repeat the experiment twice to get the average peak area values of lutein and zeaxanthin respectively. A.4.5 Result calculation The lutein content is calculated as the mass fraction w1 of lutein; the value is expressed in %, and calculated according to Formula (A.2): A.5.3.1 Headspace bottle temperature: 80 °C. A.5.3.2 Quantitative loop temperature: 85 °C. A.5.3.3 Transmission line temperature: 100 °C. A.5.3.4 Headspace bottle equilibrium time: 40.0 min. A.5.3.5 Gas cycle time: 30.0 min. A.5.3.6 Pressurization time: 0.2 min. A.5.3.7 Quantitative loop filling time: 0.2 min. A.5.3.8 Quantitative loop equilibrium time: 0.05 min. A.5.3.9 Sampling time: 1.0 min. A.5.3.10 Headspace bottle pressure: 95.15 kPa. A.5.4 Analysis steps A.5.4.1 Preparation of control solution Precisely draw 1.5 μL of n-hexane in a 100 mL volumetric flask; use dimethylformamide (DMF) to dilute to the mark; shake well; draw 25.0 mL to a 50 mL volumetric flask; use DMF to fix volume. This is the control solution (in the control solution, the alkane concentration is 0.004 95 mg/mL). Sensitivity solution preparation: Draw 1.0 mL of the control solution into a 10 mL volumetric flask; use DMF to fix volume; shake well (the concentration of n- hexane in the sensitivity solution is 0.000 495 mg/mL). A.5.4.2 Preparation of sample solution Accurately weigh about 300 mg of the sample; place it in a 10 mL headspace bottle; add 3.0 mL of DMF to dilute; shake well. A.5.4.3 System suitability requirements Take 3.0 mL of the above control solution of A.5.4.1 in a 10 mL headspace bottle; continuously inject 6 times. The number of theoretical plates of the main peak shall not be less than 5 000; the peak area RSD shall not be greater than 10.0%; the signal-to-noise ratio of the main peak in the sensitivity solution shall not be less than 3. A.5.5 Result calculation ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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