GB/T 24253-2009 PDF in English
GB/T 24253-2009 (GB/T24253-2009, GBT 24253-2009, GBT24253-2009)
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Textiles -- Evaluation for anti-mites activity
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GB/T 24253-2009: PDF in English (GBT 24253-2009) GB/T 24253-2009
GB
ICS 59.080.30
W 04
NATIONAL STANDARD OF THE
PEOPLE'S REPUBLIC OF CHINA
Textiles - Evaluation for anti-mites activity
ISSUED ON. JUNE 19, 2009
IMPLEMENTED ON. FEBRUARY 01, 2010
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Terms and definitions ... 4
4 Safety precautions ... 5
5 Principle ... 5
6 Equipment and materials ... 5
7 Test mites ... 6
8 Sample preparation ... 6
9 Test methods and procedures ... 7
10 Calculation and evaluation of results ... 9
11 Test report ... 10
Foreword
Annex A of this Standard is informative.
The Standard was proposed by China Textile Industry Association.
This Standard shall be under the jurisdiction of the Basic Standards Subcommittee of
National Standardization Technical Committee on Textiles (SAC/TC 209/SC 1).
Drafting organizations of this Standard. Shenzhen Kangyi Health Products Co., Ltd.,
Beijing Jlsun tech Co., Ltd., Standardization Institute of Textile Industry, Guangdong
Provincial Institute of Microbiology.
Main drafters of this Standard. Shang Chengjie, Fang Xijiang, Xie Xiaobao, Jia
Jiaxiang, Li Ya, Zhang Jintong, Zhang Hongjie, Wang Xingfu, Ouyang Yousheng.
Textiles - Evaluation for anti-mites activity
1 Scope
This Standard specifies the test and evaluation for textiles’ anti-mites activity by
repellent method and inhibition method.
This Standard applies to various kinds of textiles such as feather, fibers, yarns, fabrics
and articles. Wherein repellent method applies to all textile products; inhibition method
is suitable for infrequently-washed products, for example, fillers (cotton, feather, etc.)
and carpets.
This Standard does not involve evaluation for anti-mites product safety.
2 Normative references
The following normative documents contain provisions which, through reference in this
Standard, constitute provisions of this Standard. For dated reference, subsequent
amendments to (excluding correction to), or revisions of, any of these publications do
not apply. However, all parties coming to an agreement according to this Standard are
encouraged to study whether the latest editions of these documents are applicable.
For undated references, the latest editions apply.
GB/T 12490-2007 Textiles - Tests for colour fastness - Colour fastness to domestic
and commercial laundering (ISO 105-C06.1994, MOD)
3 Terms and definitions
The following terms and definitions apply to this Standard.
3.1
Mites
A kind of tiny animal that belongs to Arthropoda, Arachnida, Acari, with the body of
small spherical or elongated shape. The basic structure is divided into two parts. jaw
and body. Mites at adult and nymph stage have four pairs of legs, the larvae have three
pairs of legs.
Note. The mites in this Standard are dust mites that can cause human allergic reactions.
3.2
Anti-mites activity
Activity of products to repellent mites or inhibit the growth and reproduction of mites.
3.3
Control sample
Materials that are used to validate the growth conditions of test mites; it shall use the
same materials with the sample but without anti-mites finishing. If necessary, it can
also use unprocessed 100% cotton fabric, after being high-temperature cooked and
washed with distilled water, as the control sample.
Note. It has been proved that using cotton standard adjacent fabric for color fastness test, after
being high-temperature cooked and washed with distilled water, as a control sample is
appropriate.
4 Safety precautions
Mites are easily diffused under test condition and cause a certain harm to testing
personnel or other people, so the test shall be conducted by trained personnel in the
specified test environment.
5 Principle
PLACE the sample and the control sample on the petri dish respectively, MAKE them
be in contact with mites under specified conditions. After developed for certain time,
COUNT the number of living mites in the sample petri dishes with the control sample
petri dishes, CALCULATE the repellent rate and inhibition rate of mites according to
the test method used, so as to evaluate anti-mites activity.
6 Equipment and materials
6.1 Dissecting microscope or stereo microscope.
6.2 Constant-temperature-and-humidity incubator, temperature range is 20°C ~ 40°C;
accurate to ± 1°C; humidity range is 70% ~ 90%, accurate to ± 5%.
6.3 Petri dishes, plastic or glass, with a diameter of 58 mm and a height of 15 mm.
6.4 Covered containers of plastic, glass, ceramic or enamel material, with a side length
of about 200 mm ~ 300 mm and a height of about 50 mm ~ 100 mm. There is a 50 mm
8.1 Sample size
Fabric. SELECT representative specimens from each sample, CUT them into a circular
with a diameter of 58 mm as a sample.
Feather, fiber, yarn, carpet. SELECT representative specimens from each sample,
CUT the fibers, yarns into short fibers with a length of 10 mm ~ 30 mm, WEIGH 0.40
g ± 0.05 g as a sample.
TAKE respectively 3 samples and 3 control samples.
8.2 Sample pretreatment
PLACE the sample at dry heat condition of 65°C ± 5°C for 10 min.
8.3 Sample washing
If samples’ anti-mites and washability activity is to be tested, WASH the 3 samples on
8.1 according to the test condition A1M in GB/T 12490-2007; one cycle is equivalent
to 5 times of washing (the specific operation of one cycle. ADD 10 steel balls in 150
mL of solution, WASH at 40°C for 45 min, REMOVE the sample, WASH twice in 100
mL of 40°C water, each for 1 min). After reaches specified number of washing times,
sufficiently wash the samples with water and then dry them.
9 Test methods and procedures
9.1 Repellent method
9.1.1 PUT a piece of sponge with a thickness of 10 mm and a side length of about 200
mm INTO a covered container, ADD a suitable amount of saturated brine (6.10) (the
height of water just immerses the sponge).
9.1.2 TAKE 7 perti dishes. PUT a petri dish in the center of the sticky board as the
central petri dish, the remaining 6 petri dishes are placed evenly around the central
petri dish in petal shape; STICK the edges between each dish with transparent tape of
the same width (as a bridge). Then attach 7 petri dishes on the sticky board.
9.1.3 In the 6 petri dish around, PUT respectively sample and control samples at
intervals. LAY the samples ON the bottom of the petri dishes uniformly, smoothly and
tightly, and PUT 0.05 g of mite feed in the center of the samples.
9.1.4 PLACE (2000 ± 200) living mites IN the central dish.
9.1.5 PLACE the sticky board assembly that has been placed with test mites and feed
ON the sponge (see Figure 1), COVER the container, PLACE it in a constant-
temperature-and-humidity incubator at a temperature of (25 ± 2)°C and a relative
Annex A
(Informative)
Nutrients of mites feed
The nutrients of the mites feed used in the same test shall be unified. Feed shall be
avoided to have impurities that contains insecticide. Table A.1 gives the examples of
mites feed nutrients. When using beer yeast in the feed, the amount shall not be more
than 15%. The particle size diameter of feed shall not exceed 100 μm.
Table A.1
Items Food 1 Food 2 Food 3 Food 4 Food 5
Constituent
Fish or fish by-products Ο Ο × × ×
Cereals Ο Ο × × Ο
Yeast Ο Ο × × Ο
Milk or dairy × Ο × × ×
Egg or egg products Ο Ο × × ×
Vegetable protein extracts × Ο × × Ο
Vegetables × Ο × × Ο
Meat or animal by-products × Ο × × ×
Molluscs or crustaceans Ο Ο × × ×
Grease or fat Ο × × × ×
Seaweed Ο Ο × × Ο
Sugar Ο × × × ×
Wheat germ × × Ο Ο ×
Beer yeast × × Ο × ×
Brown beer yeast × × × Ο ×
By-products of original plants × Ο × × Ο
Minerals × × × × Ο
Average
analysis
Natural protein 48% 43% 32%
Natural fat 9% 7% 6%
Ashes 11% 9% 6%
Natural cellulose 2% 2% 2%
Moisture 6% 7.5% 6%
Vitamin (per
kilogram)
Vitamin A 37600 IU 26000 IU 29295 IU
Vitamin D3 2000 IU 1700 IU 1830 IU
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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