GB/T 5009.194-2003 English PDF
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GB/T 5009.194-2003: Determination of immunoglobulin in health foods
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT5009.194-2003GB National Standard of the People's Republic of China Determination of immunoglobulin in health foods Issued on. MARCH 26, 2010 Implemented on. JUNE 1, 2010 Issued by. Ministry of Health of the People’s Republic of China; China National Standardization Management Committee
Table of Contents
For Information Only – GB 5009 & GB 5413 Series Chinese Standards... 3 Foreword... 21 Introduction... 22 1 Application scope... 23 2 Principle... 23 3 Reagents... 23 4 Instruments and devices... 23 5 Analysis steps... 23 6 Calculation result... 24 7 Precision... 24 8 Chromatograms... 25Foreword
This Standard is proposed and administered by Ministry of Health, the People's Republic of China. This Standard is responsibly drafted by Food Hygiene Supervision and Inspection Institute of Ministry of Health. Main drafters of this standard. Yang Zuying, and Song Shufeng.1 Application scope
This Standard specifies the determination method of immunoglobulin IgG in health foods. This Standard applies to determination of immunoglobulin IgG in health foods such as tablets, capsules, and powders. The detection limit of this method. When taking 0.1g of the sample; diluting it to 25 mL; and the injection volume is 20μL, the detection concentration shall be 0.5 mg / mL.2 Principle
According to the principle of high performance affinity chromatography, under the condition of phosphate buffer, immunoglobulin IgG is connected with ligand. Under the condition of pH2.5 hydrochloric acid glycine, ELUTE the immunoglobulin IgG.3 Reagents
3.1 Mobile phase A. pH6.5, 0.05 mol / L phosphate buffer. 3.2 Mobile phase B. pH2.5, 0.05 mol / L glycine hydrochloric acid buffer. 3.3 IgG standard stock solution. WEIGH 0.0100g of IgG standard sample (Sigma Chemical Company), USE mobile phase A (3.1) to dissolve, and SET the volume to 10.0 mL, SHAKE well, the concentration shall be 1.0 mg / mL.4 Instruments and devices
High performance liquid chromatography instrument with UV detection and gradient elution device.5 Analysis steps
5.1 Sample treatment. WEIGH 0.1g (accurate to 0.001 g) of sample, USE mobile phase A (3.1) to dilute to 25.0mL, SHAKE well, INJECT after it is filtered through 0.45μm microporous membrane. 5.3 HPLC reference conditions Chromatogram column. Pharmacia HI-Trap Protein G column, 1 mL. Wavelength. 280 nm.6 Calculation result
Calculation result shall remain two significant figures.7 Precision
Under repeatable condition, the difference of the absolute values of two independent measurement results shall not exceed 10% of the arithmetic mean.8 Chromatograms
Figure 1 IgG standard chromatogram Figure 2 Colostrum chromatogram ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
