GB/T 5009.194-2003 PDF English
US$70.00 · In stock · Download in 9 secondsGB/T 5009.194-2003: Determination of immunoglobulin in health foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB/T 5009.194: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 5009.194-2003 | English | 70 |
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Determination of immunoglobulin in health foods
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| GB/T 5009.194-2003 | English | 70 |
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Determination of immunoglobulin in health foods
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GB/T 5009.194-2003: Determination of immunoglobulin in health foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT5009.194-2003
GB
National Standard
of the People's Republic of China
Determination of immunoglobulin in health foods
Issued on. MARCH 26, 2010
Implemented on. JUNE 1, 2010
Issued by. Ministry of Health of the People’s Republic of China;
China National Standardization Management Committee
Table of Contents
For Information Only – GB 5009 & GB 5413 Series Chinese Standards... 3
Foreword... 21
Introduction... 22
1 Application scope... 23
2 Principle... 23
3 Reagents... 23
4 Instruments and devices... 23
5 Analysis steps... 23
6 Calculation result... 24
7 Precision... 24
8 Chromatograms... 25
Foreword
This Standard is proposed and administered by Ministry of Health, the People's
Republic of China.
This Standard is responsibly drafted by Food Hygiene Supervision and
Inspection Institute of Ministry of Health.
Main drafters of this standard. Yang Zuying, and Song Shufeng.
1 Application scope
This Standard specifies the determination method of immunoglobulin IgG in health foods.
This Standard applies to determination of immunoglobulin IgG in health foods such as
tablets, capsules, and powders.
The detection limit of this method. When taking 0.1g of the sample; diluting it to 25 mL; and
the injection volume is 20μL, the detection concentration shall be 0.5 mg / mL.
2 Principle
According to the principle of high performance affinity chromatography, under the condition
of phosphate buffer, immunoglobulin IgG is connected with ligand. Under the condition of
pH2.5 hydrochloric acid glycine, ELUTE the immunoglobulin IgG.
3 Reagents
3.1 Mobile phase A. pH6.5, 0.05 mol / L phosphate buffer.
3.2 Mobile phase B. pH2.5, 0.05 mol / L glycine hydrochloric acid buffer.
3.3 IgG standard stock solution. WEIGH 0.0100g of IgG standard sample (Sigma Chemical
Company), USE mobile phase A (3.1) to dissolve, and SET the volume to 10.0 mL, SHAKE
well, the concentration shall be 1.0 mg / mL.
4 Instruments and devices
High performance liquid chromatography instrument with UV detection and gradient elution
device.
5 Analysis steps
5.1 Sample treatment. WEIGH 0.1g (accurate to 0.001 g) of sample, USE mobile phase A
(3.1) to dilute to 25.0mL, SHAKE well, INJECT after it is filtered through 0.45μm
microporous membrane.
5.3 HPLC reference conditions
Chromatogram column. Pharmacia HI-Trap Protein G column, 1 mL.
Wavelength. 280 nm.
6 Calculation result
Calculation result shall remain two significant figures.
7 Precision
Under repeatable condition, the difference of the absolute values of two independent
measurement results shall not exceed 10% of the arithmetic mean.
8 Chromatograms
Figure 1 IgG standard chromatogram Figure 2 Colostrum chromatogram
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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