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YC/T 251-2008: Tobacco and tobacco products. Determination of glucose, fructose and sucrose. Ion chromatographic method
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Tobacco and tobacco products. Determination of glucose, fructose and sucrose. Ion chromatographic method
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YC/T 251-2008
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Basic data | Standard ID | YC/T 251-2008 (YC/T251-2008) | | Description (Translated English) | Tobacco and tobacco products. Determination of glucose, fructose and sucrose. Ion chromatographic method | | Sector / Industry | Tobacco Industry Standard (Recommended) | | Classification of Chinese Standard | X85 | | Classification of International Standard | 65.160 | | Word Count Estimation | 9,940 | | Date of Issue | 2008-04-14 | | Date of Implementation | 2008-04-14 | | Quoted Standard | GB/T 5606.1; GB/T 6682; GB/T 19616; YC/T 31 | | Issuing agency(ies) | State Tobacco Monopoly Administration | | Summary | This standard specifies the tobacco and tobacco products, glucose, fructose, sucrose ion chromatographic method. This standard applies to tobacco and tobacco products, glucose, fructose, sucrose determination. | YC/T 251-2008: Tobacco and tobacco products. Determination of glucose, fructose and sucrose. Ion chromatographic method
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Tobacco and tobacco products.Determination of glucose, fructose and sucrose.Ion chromatographic method
ICS 65.160
X85
Record Number. 23590-2008
People's Republic of China tobacco industry standards
Tobacco and tobacco products, glucose, fructose,
Sucrose ion chromatography
Posted 2008-04-14
2008-04-14 implementation
State Tobacco Monopoly Bureau issued
Foreword
Appendix A of this standard is an informative annex.
This standard was proposed by the State Tobacco Monopoly Bureau.
This standard by the National Standardization Technical Committee on Tobacco (TC144) centralized.
This standard was drafted. Tobacco Guangdong Industrial Corporation, Research Center for Eco-Environmental Sciences, Shanghai Tobacco (Group) Company, Hunan
Tobacco industry.
Drafters of this standard. light Shen Lin, Hu Jing, Mou, Konghao Hui, Sun Wenliang Wu Ming-Jian, Shi Wen Zhuang, Zhang Mao excellent, Shi Yali.
Tobacco and tobacco products - Determination of glucose, fructose, sucrose
Ion Chromatography
1 Scope
This standard specifies the ion chromatographic method of tobacco and tobacco products, glucose, fructose, sucrose.
This standard applies to glucose, fructose, Tobacco and tobacco products Determination of sucrose.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 5606.1 cigarette - Part 1. Sampling
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
General principles GB/T 19616 batches of raw tobacco samples (GB/T 19616-2004, ISO 4874.2000, MOD)
Preparation and water YC/T 31 samples of tobacco and tobacco products - Determination oven method
Principle 3
Water samples were extracted glucose, fructose, sucrose, after separation by anion exchange, electrochemical detection and analysis.
4 Reagents
4.1 In addition to special requirements, should use analytical reagent, the water should be consistent with GB/T 6682 stipulated a water.
4.2 sodium hydroxide solution, 50% (mass fraction).
4.3 benzoic acid solution, 0.1% (mass fraction).
4.4 mobile phase.
--- Mobile phase A. water;
--- Mobile phase B. sodium hydroxide solution, 50mmol/L. Pipette 2.6mL50% sodium hydroxide solution (4.2) to 1000mL capacity
Volumetric flask, dilute with water to volume;
--- Mobile phase C. sodium hydroxide solution, 250mmol/L. Pipette 13.0mL50% sodium hydroxide solution (4.2) to 1000mL
Volumetric flask, dilute with water to volume;
--- Mobile phase D. sodium acetate solution, 1mol/L. Weigh 41.02g sodium acetate, dissolved in water, and then transferred to 500mL volumetric flask
, Diluted with water to volume.
4.5 single standard stock solution. Weigh 0.1g of glucose, fructose, sucrose, accurate to 0.0001g, 0.1% benzoic acid solution
(4.3) was dissolved, and then were transferred to 100mL volumetric flask, with 0.1% benzoic acid solution (4.3) to the mark, formulated at a concentration of about
1000μg/mL stock solution of a single standard. Stored at 4 ℃ conditions, it should be used within one month.
4.6 mixed standard stock solution. Pipette 10.0mL are glucose, fructose, sucrose single standard stock solution (4.5) in a 100mL container
Flask, with 0.1% benzoic acid solution (4.3) diluted to the mark, formulated as a concentration of about 100μg/mL of glucose, fructose, sucrose,
Mixed standard stock solution.
4.7 Mixed Series Working standard solution. Pipette a certain volume were mixed standard stock solution (4.6) in 50mL volumetric flask, with 0.1% of benzene
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