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SN/T 4736-2016: Qulitative PCR method for detecting genetically modified component in cows and their derived products
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4736-2016 | English | 229 |
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Qulitative PCR method for detecting genetically modified component in cows and their derived products
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SN/T 4736-2016
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Basic data | Standard ID | SN/T 4736-2016 (SN/T4736-2016) | | Description (Translated English) | Qulitative PCR method for detecting genetically modified component in cows and their derived products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Word Count Estimation | 10,147 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4736-2016: Qulitative PCR method for detecting genetically modified component in cows and their derived products
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Identification of specific transgenic components in cattle and their products)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Specific genetically modified ingredients in cattle and their products
Qualitative PCR detection method
Released on December 12,.2016
2017-07-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. China Academy of Inspection and Quarantine, China Agricultural University, Shandong Entry and Exit Inspection
Epidemic situation.
The main drafters of this standard. Lin Xiangmei, Wang Qin, Li Xiaolin, Zhu Zhenying, Liu Jian, Wang Jianwu, Sun Min, Fu Wei, Liu Xiaofei, Mei Lin, Qiu Songyu,
Liu Dandan.
Specific genetically modified ingredients in cattle and their products
Qualitative PCR detection method
1 Scope
This standard specifies qualitative PCR detection methods for specific transgenic components in cattle and their products.
This standard applies to the qualitative detection of transfer of human lactoferrin gene, transfer of human α-lactalbumin gene and transfer of lysozyme gene to cattle and its products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Qualitative polymerase chain reaction (PCR) method for qualitative detection of bovine and sheep-derived components in GB/T.20190 feed
3 Abbreviations
The following abbreviations apply to this document.
hLA. human whey protein (HumanLactobumin)
hLF. Human Lactoferrin
hLY. human lysozyme (HumanLysozyme)
PCR. Polymerase Chain Reaction (PolymeraseChainReaction)
TE. Tris-HCl, EDTA buffer
Tris. Tris(hydroxymethyl)aminomethane
4 qualitative PCR test
4.1 Main instruments
4.1.1 PCR instrument.
4.1.2 Ultra-clean workbench.
4.1.3 Sterilization pot.
4.1.4 Gel imaging system.
4.1.5 Nucleic Acid Protein Analyzer.
4.1.6 High-speed refrigerated centrifuge, bench-top small centrifuge, Mini personal centrifuge.
4.1.7 Low temperature refrigerator, refrigerator freezer.
4.1.8 Water purifier, double steamer.
4.1.9 Vortex oscillator.
4.1.10 Electrophoresis apparatus.
4.2 Primary reagents
Taq DNA polymerase, agarose (electrophoresis pure), chloroform, isopropanol, isoamyl alcohol, 70% ethanol, TE buffer, 50 times TAE
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