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SN/T 4737-2016: Protocol of the real-time PCR for detecting genetically modified component in pigs and their derived products
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Protocol of the real-time PCR for detecting genetically modified component in pigs and their derived products
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SN/T 4737-2016
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Basic data | Standard ID | SN/T 4737-2016 (SN/T4737-2016) | | Description (Translated English) | Protocol of the real-time PCR for detecting genetically modified component in pigs and their derived products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Word Count Estimation | 10,119 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4737-2016: Protocol of the real-time PCR for detecting genetically modified component in pigs and their derived products
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the real-time PCR for detecting genetically modified component in pigs and their derived products
China's entry-exit inspection and quarantine industry standards
Specific genetically modified components in pigs and their products
Real-time quantitative PCR detection method
Published on.2016-12-12
2017-07-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. China Academy of Inspection and Quarantine, Chinese Academy of Agricultural Sciences, Beijing Institute of Animal Husbandry and Veterinary, Chinese People
Shandong Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Lin Xiangmei, Wang Qin, Li Xiaolin, Zhu Zhenying, Liu Jian, Li Kui, Pan Dengke, Sun Min, Fu Wei, Liu Xiaofei, Mei Lin,
Liu Dandan and Qi Songsong.
Specific genetically modified components in pigs and their products
Real-time quantitative PCR detection method
1 Scope
This standard specifies real-time quantitative PCR detection methods for specific transgenic components in pigs and their products.
This standard applies to transfatty acid desaturase gene, transphytase gene, myostatin gene knockout pigs and their products.
Testing.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB/T 19495.2 Laboratory Testing Requirements for Genetically Modified Products
3 Abbreviations
The following abbreviations apply to this document.
APPA. Phytase
MSTN. Myostatin
PCR. Polymerase Chain Reaction (Polymerase Chain Reaction)
sFat-1. w-3 fatty acid desaturase gene (omega-3 fatty acid desaturase)
TE. Tris-HCl, EDTA buffer
4 Anti-pollution measures
The anti-pollution measures of real-time quantitative PCR detection of specific transgenic components in pigs and their products should comply with GB/T 19495.2
Provisions.
5 Real-time quantitative PCR test
5.1 The main instrument
5.1.1 Real-time fluorescence quantitative PCR instrument.
5.1.2 Clean Benches.
5.1.3 Sterilization pot.
5.1.4 Nucleic acid protein analyzer.
5.1.5 High-speed refrigerated centrifuge, bench-top small centrifuge, Mini personal centrifuge.
5.1.6 low temperature refrigerator, refrigerator freezer.
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