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SN/T 2615-2010: Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2615-2010 | English | 239 |
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Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch
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SN/T 2615-2010
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Basic data | Standard ID | SN/T 2615-2010 (SN/T2615-2010) | | Description (Translated English) | Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,925 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Apple side rot phytosanitary quarantine and identification of bacteria. This standard applies to apples and pears into the exit side of apple fruit rot quarantine and identification. | SN/T 2615-2010: Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phialophora malorum (Kidd
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Apple side Rot quarantine and identification methods
DetectionandidentificationofPhialophoramalorum (Kidd
McColoch
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Xinjiang People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
Zhejiang Exit Inspection and Quarantine and the State.
Drafters of this standard. Wu Shan goods, Du Hongzhong, Wang Wen-cheng, Wu Zhiyi, Yan Jin.
Apple side Rot quarantine and identification methods
1 Scope
This standard specifies the identification of phytosanitary quarantine in Apple's side Rot.
This standard applies to entry and exit quarantine and identification of apples and pears apples rot bacteria edge.
Principle 2
Apple side Rot symptoms in the host, morphology and molecular biological characteristics of the main basis for the development of this standard.
3 apparatus and appliances
3.1 Instrument
Microscope (with measurements), stereo microscope, clean benches, light biological incubator, scales, autoclaves, high-speed desktop
Centrifuges, pH meter, water bath, PCR amplification device, electrophoresis, gel imager.
3.2 Appliances
Beakers, flasks, graduated cylinder, test tubes, Petri dishes (diameter 9cm), slides, cover slips, alcohol lamp, plastic pestle, pipettes, pipette
Pipette tips, centrifuge tubes, liquid nitrogen tank.
4 Reagents and culture medium
4.1 Reagents
Agar, potato, glucose, lactate, agarose, sterile double distilled water, liquid nitrogen, Tris-HCl, magnesium oxide, hydrochloric acid, EDTA, hydroxide
Sodium acetate, sodium chloride, potassium chloride, Tris, CTAB, TAE, ethanol, phenol, ethidium bromide (EB), chloroform, isopropanol, isoamyl alcohol, protein
Enzyme K, TaqDNA polymerase, dNTP, DNA molecular weight Marker, PCR primers PMA-1 and PMA-2, real-time fluorescence
PCR primers PMA-P1, PMA-P2 and probe PMA-Probe-457.
4.2 Medium
Potato dextrose medium (PDA). potato 200g, dextrose 20g, Agar 20g, Distilled water 1000mL, sterilization.
Acidic potato dextrose medium (APDA). Preparation 1000mLPDA, after sterilization was cooled to 50 ℃, added 1.5mL85%
Lactic acid.
5 identification methods
5.1 Symptom Check
Disease symptoms in Appendix A.
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