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SN/T 2327-2009: Determination of canthaxanthin and astaxanthin in foods of animal origin food for import and export
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Determination of canthaxanthin and astaxanthin in foods of animal origin food for import and export
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SN/T 2327-2009
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Basic data | Standard ID | SN/T 2327-2009 (SN/T2327-2009) | | Description (Translated English) | Determination of canthaxanthin and astaxanthin in foods of animal origin food for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.120.01 | | Word Count Estimation | 21,235 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of foodstuffs of animal origin canthaxanthin, astaxanthin HPLC and LC MS/MS confirmatory method. This standard applies to fish, eel, chicken, eggs, duck liver, kidney and milk canthaxanthin, astaxanthin determination and confirmation. | SN/T 2327-2009: Determination of canthaxanthin and astaxanthin in foods of animal origin food for import and export
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Determination of canthaxanthin and astaxanthin in foods of animal origin food for import and export
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food of animal origin canthaxanthin,
Detection of astaxanthin
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B and Appendix C are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. I Contier, Jiang Qian, Liu Cai, Yang Fang, Huang Jie, Liyao Ping, Li Jie.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food of animal origin canthaxanthin,
Detection of astaxanthin
1 Scope
This standard specifies the HPLC and LC import and export food of animal origin canthaxanthin, astaxanthin - MS/MS
Confirmatory method.
This standard applies to fish, eel, chicken, eggs, duck liver, kidney and milk canthaxanthin, astaxanthin determination and confirmation.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
Samples were extracted with acetonitrile, hexane, and concentrated, and high performance liquid chromatography detection, external standard. And if necessary liquid
Chromatography - mass spectrometry/mass spectrometry confirmation.
4 Reagents and materials
All reagents unless otherwise specified, were of analytical grade water as a water GB/T 6682 regulations.
4.1 acetonitrile. chromatography.
4.2 hexane. chromatographically pure.
4.3 n-propanol.
4.4 formic acid.
4.5 tert-butyl-p-cresol (BHT). chemically pure.
4.6 anhydrous sodium. 650 ℃ burning 4h, stored in a desiccator, cool reserve.
4.7 ammonium acetate.
4.8 acetonitrile extraction agent. 0.25gBHT dissolved in 500mL acetonitrile.
4.9 BHT- acetonitrile. 5gBHT was dissolved in 500mL acetonitrile.
4.10 ammonium acetate (5mmol/L, containing 0.2% formic acid). weigh 0.38g ammonium acetate, dissolved in water dilution water, add 2.0mL of formic acid,
Add water to 1.0L.
4.11 canthaxanthin standards. canthaxanthin, CAS. 514-78-3, a purity of greater than or equal to 90% at 4 ℃ dark storage.
4.12 astaxanthin standard. astaxanthin, CAS. 472-61-7, a purity of greater than or equal to 90%, dark storage at -18 ℃.
4.13 standard stock solution. namely, accurately weighed amount (equivalent to 5.00mg pure, accurate to 0.01mg) canthaxanthin, astaxanthin standard,
BHT- were added to acetonitrile solution (4.9) dissolved in 200mL volumetric flask brown, preparation of standard storage at a concentration of 25.0μg/mL of
Preparation of liquid. Nitrogen purge set -18 ℃ dark preservation, use two weeks.
4.14 mixed standard working solution. accurate Pipette were shaken canthaxanthin, astaxanthin standard stock solution, mixed and diluted into appropriate and accurate with acetonitrile
Concentration, ie with the box.
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