SN/T 2014: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2014-2014 | English | 639 |
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Detection and identification of Tomato black ring virus
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SN/T 2014-2014
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| SN/T 2014-2007 | English | 479 |
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Tomato black ring virus quarantine and identification methods
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SN/T 2014-2007
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PDF similar to SN/T 2014-2014
Basic data | Standard ID | SN/T 2014-2014 (SN/T2014-2014) | | Description (Translated English) | Detection and identification of Tomato black ring virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 16,172 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Older Standard (superseded by this standard) | SN/T 2014-2007 | | Quoted Standard | SN/T 1840 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the tomato black ring virus quarantine and identification methods. This standard applies to quarantine and identification of plant seeds, seedlings, tissue culture, vegetative propagation material Tomato black ring virus. | SN/T 2014-2014: Detection and identification of Tomato black ring virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Tomato black ring virus quarantine and identification methods)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 2014-2007
Tomato black ring virus quarantine and identification methods
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 2014-2007 "black ring virus quarantine and identification of tomato."
This standard compared with SN/T 2014-2007, the main technical changes are as follows.
--- Increased IC-RT-PCR detection method;
--- Added Appendix A tomato black ring virus Introduction.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shanghai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters. Zhang Yujun, Guo Jingze, Yang Cuiyun, Wang Jincheng, Liu Peng, Liu Fang, Liuyue Ting, Xiahui Juan, Roca phoenix, to Tsui.
This standard replaces the standards previously issued as follows.
--- SN/T 2014-2007.
Tomato black ring virus quarantine and identification methods
1 Scope
This standard specifies the tomato black ring virus quarantine and identification methods.
This standard applies to quarantine and identification of plant seeds, seedlings, tissue culture, vegetative propagation material Tomato black ring virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1840 plant virus immune electron microscopy method
3 Tomato black ring virus basic information
Name. Tomatoblackringvirus
Abbreviation. TBRV
Taxonomic position. Associated cowpea virus family (Secoviridae), nematodes pass polyhedron virus genus (Nepovirus).
Transmission. The virus by nematodes (Longidorusattenuatus and L.elongatus) transmission, mechanical transmission, seeds (and chickweed
Soybeans, etc.) can also be transmitted by vegetative propagation material transport over long distances.
For additional information, see Appendix A. Tomato black ring virus
Principle 4
Biological Characteristics of Tomato black ring virus, serological properties, molecular biology and mitochondrial morphological characteristics (see Appendix A) is to identify the virus
Basis. Using DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay), immune electron microscopy, RT-PCR (reverse transcriptase polymerase chain reaction
Should), IC-RT-PCR (reverse transcriptase polymerase chain reaction immuno-capture) Tomato black ring virus was detected. Using technology to make detection more
As a result, a comprehensive judgment whether the sample with tomato black ring virus.
5 equipment, facilities and appliances
5.1 Equipment
Microplate reader, electronic balance (1/10000g), PCR instrument, electrophoresis, gel imaging system, micro juicer, water bath, Autoclave,
Water meter, a microwave, a magnetic stirrer, a mortar and pestle, low speed centrifuge, high-speed refrigerated centrifuge, bio-incubator, low temperature refrigerator and -80 ℃
Ultra-low temperature freezer.
5.2 Facilities
Quarantine greenhouse (10 ℃ ~ 30 ℃).
5.3 Appliances
Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and supporting tip, ELISA plates, tubes, RESEARCH
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