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GB/T 38479-2021: Determination of chitosan content - High performance liquid chromatography
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| GB/T 38479-2021 | English | 159 |
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Determination of chitosan content - High performance liquid chromatography
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GB/T 38479-2021
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Basic data | Standard ID | GB/T 38479-2021 (GB/T38479-2021) | | Description (Translated English) | Determination of chitosan content - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Word Count Estimation | 7,748 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38479-2021: Determination of chitosan content - High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chitosan content -- High performance liquid chromatography
ICS 07:080
CCSA21
National Standards of People's Republic of China
Determination of Chitosan Content by High Performance Liquid Chromatography
Published on 2021-12-31
2022-07-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents"
drafted:
Please note that some content of this document may be patented: The issuing agency of this document assumes no responsibility for identifying patents:
This document is proposed and managed by China National Institute of Standardization:
This document was drafted by: Yantai Institute of Coastal Zones, Chinese Academy of Sciences, Institute of Oceanography, Chinese Academy of Sciences, Shandong Luhai Lansheng Biotechnology
Co:, Ltd:, Shandong Weikang Biomedical Technology Co:, Ltd:, Qingdao University of Science and Technology, China National Institute of Standardization, Chinese Academy of Sciences Process Engineering
Cheng Research Institute:
The main drafters of this document: Guo Zhanyong, Dong Fang, Qin Song, Miao Qin, Qin Rongji, Cui Yulin, Li Pengcheng, Yang Xihong, Li Jinguo, Xing Rong'e,
Ma Aijin, Chen Jun, Huang Yongdong:
Determination of Chitosan Content by High Performance Liquid Chromatography
1 Scope
This document specifies a method for the determination of chitosan content by high performance liquid chromatography:
This document applies to the determination of chitosan content:
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document:
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Terms and Definitions
The following terms and definitions apply to this document:
3:1
Chitosan
Chitin removes more than 55% of the N-acetyl group:
4 Principles
Chitosan was acetylated into chitin and then acid hydrolyzed, and the concentration of glucosamine hydrochloride in the hydrolyzed solution was detected by high performance liquid chromatography:
The content of chitosan in the sample was quantitatively calculated by external standard method:
5 Reagents or materials
5:1 Water
Grade 1 water specified in GB/T 6682:
5:2 Acetonitrile
Chromatographically pure:
5:3 Hydrochloric acid
The quality score is 36%~38%:
5:4 Nitrogen
Purity ≥99:9%:
5:5 Glucosamine hydrochloride
Purity ≥99%:
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