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US$159.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 38478-2021: Determination of astaxanthin optical isomers content - Liquid chromatography Status: Valid
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Determination of astaxanthin optical isomers content - Liquid chromatography
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GB/T 38478-2021
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Basic data | Standard ID | GB/T 38478-2021 (GB/T38478-2021) | | Description (Translated English) | Determination of astaxanthin optical isomers content - Liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Word Count Estimation | 7,746 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 38478-2021: Determination of astaxanthin optical isomers content - Liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of astaxanthin opticalisomers content -- Liquid chromatography
ICS 07:080
CCSA21
National Standards of People's Republic of China
Determination of optical isomer content of astaxanthin
liquid chromatography
Published on 2021-12-31
2022-07-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents"
drafted:
This document is proposed and managed by China National Institute of Standardization:
This document is drafted by: Yantai Institute of Coastal Zones, Chinese Academy of Sciences, Institute of Oceanography, Chinese Academy of Sciences, China National Institute of Standardization, Shandong
Provincial Institute of Standardization, Yunnan Longbray Biotechnology Co:, Ltd:, Fujian Normal University Fuqing Branch, and Institute of Process Engineering, Chinese Academy of Sciences:
The main drafters of this document: Liu Jianguo, Qin Song, Su Fang, Han Chunmei, Liang Wenwei, Cui Yulin, Ma Aijin, Chen Jun, Wang Yinchu, Huang Yongdong:
Determination of optical isomer content of astaxanthin
liquid chromatography
1 Scope
This document specifies a method for the determination of optical isomer content in astaxanthin by liquid chromatography:
This document is applicable to the determination of the optical isomer content of astaxanthin in astaxanthin products:
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document:
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Terms and Definitions
There are no terms and definitions that need to be defined in this document:
4 Principles
After grinding and leaching the astaxanthin in the sample, it was enzymatically hydrolyzed by cholesterol esterase to obtain free astaxanthin, which was separated by a chiral chromatographic column and used to obtain free astaxanthin:
It was determined by liquid chromatograph equipped with UV detector and quantified by external standard method:
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used:
5:1 Water
It should comply with the secondary water regulations in GB/T 6682:
5:2 Acetonitrile
Chromatographically pure:
5:3 Cholesterol esterase
Enzyme activity ≥ 10U/mg:
5:4 Methyl tert-butyl ether
Chromatographically pure:
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