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GB/T 38482-2021: Determination of animal type I collagen - Polyacrylamide gel electrophoresis
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| GB/T 38482-2021 | English | 139 |
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Determination of animal type I collagen - Polyacrylamide gel electrophoresis
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GB/T 38482-2021
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Basic data | Standard ID | GB/T 38482-2021 (GB/T38482-2021) | | Description (Translated English) | Determination of animal type I collagen - Polyacrylamide gel electrophoresis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Word Count Estimation | 6,690 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38482-2021: Determination of animal type I collagen - Polyacrylamide gel electrophoresis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of animal type Ⅰ collagen -- Polyacrylamide gel electrophoresis
ICS 07:080
A21
National Standards of People's Republic of China
Determination of animal-derived type I collagen composition
polyacrylamide gel electrophoresis
Published on 2021-12-31
2022-07-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard is proposed and managed by China National Institute of Standardization:
This standard was drafted by: Yantai Institute of Coastal Zones, Chinese Academy of Sciences, China National Institute of Standardization, Shanghai Ocean University, China Ocean University
Science, Shandong Hongbo Standardization Service Office, and Institute of Process Engineering, Chinese Academy of Sciences:
The main drafters of this standard: Qin Song, Li Jie, Wu Wenhui, Hou Hu, Ma Aijin, Cui Yulin, Chen Jun, Han Chunmei, Huang Yongdong:
Determination of animal-derived type I collagen composition
polyacrylamide gel electrophoresis
1 Scope
This standard specifies a method for determining the composition of animal-derived type I collagen by polyacrylamide gel electrophoresis (SDS-PAGE):
This standard applies to the determination of type I collagen components in animal-derived collagen products:
2 Normative references
The following documents are essential for the application of this document: For dated references, only the dated version applies to this article
pieces: For undated references, the latest edition (including all amendments) applies to this document:
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
Pharmacopoeia of the People's Republic of China (2015 Edition)
3 Terms and Definitions
The following terms and definitions apply to this document:
3:1
Collagen
Three peptide chains of left-handed helical repeating Gly-XY sequence prepared from purified (non-crosslinked) type I collagen
Structural protein coiled into a right-handed supercoiled structure characteristic:
Note: Gly is glycine, X is usually proline, and Y is usually hydroxyproline or hydroxylysine:
3:2
typeⅠcollagen
Collagen with a complete triple helix structure composed of 2 α1 chains and 1 α2 peptide chain:
4 Principles
The use of type I collagen has a triple helix structure that other proteins do not have, and with the action of specific collagenase, through Coomassie
Brilliant blue was used to determine the staining limit of bovine serum albumin (BSA), and the composition of type Ⅰ collagen was determined by SDS-PAGE:
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used:
5:1 Bacterial collagenase:
5:2 Bovine serum albumin (BSA) standard:
5:3 Water:
It should meet the first-class water regulations in GB/T 6682:
5:4 20mmol/L sodium dihydrogen phosphate solution, pH7:4:
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