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GB/T 35541-2017: pfu DNA polymerase
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35541-2017 | English | 279 |
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pfu DNA polymerase
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GB/T 35541-2017
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PDF similar to GB/T 35541-2017
Standard similar to GB/T 35541-2017 GB/T 41907 GB/T 35540 GB/T 35539 Basic data | Standard ID | GB/T 35541-2017 (GB/T35541-2017) | | Description (Translated English) | pfu DNA polymerase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,150 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35541-2017: pfu DNA polymerase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
pfu DNA polymerase
ICS 07.080
C27
National Standards of People's Republic of China
pfu DNA polymerase
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 technical requirements
5 test method 2
6 packaging, transportation and storage 2
7 shelf life 2
Appendix A (Normative) pfuDNA polymerase enzyme activity test 3
Appendix B (Normative) Exonuclease assay 6
Appendix C (Normative) Endonuclease assay 8
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized.
This standard was drafted. Xiamen Zhi Shan Biological Technology Co., Ltd., Fujian Hua Can Pharmaceutical Co., Ltd., Fujian South Health Technology Co., Ltd.
Division, Shenzhen Huaneng Kang Gene Technology Co., Ltd., Huacan Nansheng (Xiamen) Biotechnology Co., Ltd., Institute of Microbiology, Chinese Academy of Sciences, Xiamen
University, Beijing University of Chemical Technology, Shandong University, Fudan University, Beaver Nano Technology (Suzhou) Co., Ltd., Shanghai Boshi Biological Technology Co., Ltd.,
Angel Yeast Co., Ltd., China Agricultural University, Fujian Agriculture and Forestry University, Shanghai 100 赛 Biological Technology Co., Ltd.
The main drafters of this standard. Song Najie, Huangfa Can, Zheng Dengzhong, He Changhua, Zhan Xuexiong, Li Qing Court, Zhao Jing, Li Quanhong, Sheng Tong,
Chen Jinchun, Chen Xiulan, Zhong Jiang, Liu Bin, Yao Juan, Ren Hui, Xing Zhigang, Huang Fa, Zhang Xying, Teresa Teng, Zhang Lili.
Introduction
The pfuDNA polymerase is derived from Pyrococcus fariosus and has a very high thermostability and fidelity and is widely applied
For routine molecular biology experiments. Development of pfu DNA polymerase national standards to promote the industrialization of such tools, enzymes, pfu
DNA polymerase production and use of great significance.
pfu DNA polymerase
1 Scope
This standard specifies the technical requirements pfuDNA polymerase, test methods, packaging, transportation, storage and shelf life.
This standard applies to genetically engineered bacteria made of pfuDNA polymerase.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
One from Pyrococcus furiosus, molecular weight of 90ku, with high thermal stability and fidelity
DNA polymerase. This enzyme catalyzes the polymerization of deoxyribonucleotides onto the primers from the 5'-3 'direction of the template, with 3'-5' exonuclease activity, none
5'-3 'exonuclease activity.
3.2
Using a synthetic hairpin-type oligonucleotide sequence as a template/primer, 0.14 nmol of deoxynucleotide was polymerized into
The amount of enzyme required in double stranded DNA is 1 viable unit (U).
4 technical requirements
4.1 appearance
Clear transparent liquid, no sediment.
4.2 enzyme activity
≥5000U/mL.
4.3 Impurities
It should not contain exonucleases and endonucleases.
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