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GB/T 21927-2008 English PDF

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GB/T 21927-2008: Determination of Tertiary Butylhydroquinone in foods -- High-performance of liquid chromatography
Status: Obsolete
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GB/T 21927-2008English399 Add to Cart 3 days [Need to translate] Determination of Tertiary Butylhydroquinone in foods -- High-performance of liquid chromatography Obsolete GB/T 21927-2008

PDF similar to GB/T 21927-2008


Standard similar to GB/T 21927-2008

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Basic data

Standard ID GB/T 21927-2008 (GB/T21927-2008)
Description (Translated English) Determination of Tertiary Butylhydroquinone in foods -- High-performance of liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X40
Classification of International Standard 67.050
Word Count Estimation 6,686
Date of Issue 2008-05-16
Date of Implementation 2008-11-01
Regulation (derived from) National Standard Approval Announcement 2008 No.9 (Total No.122)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the tertiary butyl hydroquinone foods high performance liquid chromatography-fluorescence detector method. This standard applies to edible oils and fats, fried foods, dried fish products, biscuits, noodles, quick cooking rice, determination of TBHQ in foods such as pickled meat, dried fruit, canned. This standard types of food in TBHQ detection limit of 0. 05��g/mL.

GB/T 21927-2008: Determination of Tertiary Butylhydroquinone in foods -- High-performance of liquid chromatography


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Tertiary Butylhydroquinone in foods. High-performance of liquid chromatography ICS 67.050 X40 National Standards of People's Republic of China Determination in foods tertiary butyl hydroquinone High Performance Liquid Chromatography Posted 2008-05-16 2008-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China, China National Standardization Management Commission. This standard by the Standardization Administration of China. This standard was drafted. National Agricultural Processed Food Quality Supervision and Inspection Center, Anhui national agricultural standardization and monitoring center. The main drafters of this standard. Lu industry move, Gu Liang, Liu Kun, Chen Ge, Liu Qinfang, Chen Honglin, Jiang Toshiki. Determination in foods tertiary butyl hydroquinone High Performance Liquid Chromatography

1 Scope

This standard specifies the tert-butyl hydroquinone (TBHQ) in foods high performance liquid chromatography - fluorescence detector method. This standard applies to edible oils and fats, fried foods, dried fish products, biscuits, instant noodles, quick cooking rice, dried fruit, canned, preserved meat and other foods Determination of TBHQ. This standard various foods TBHQ detection limit was 0.05μg/mL (injection volume 10μL). Principle 2 This standard was extracted with acetonitrile foods TBHQ, defatted with n-hexane. Test solution with acetonitrile and 1.5% acetic acid as the eluent, dried Reversed phase C18 column, fluorescence detection, 32 min.

3 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade, distilled water or equivalent purity water. 3.1 tertiary butyl hydroquinone (TBHQ) Standard. Purity ≥98%. 3.2 L- ascorbyl palmitate. 3.3 n-hexane. 3.4 glacial acetic acid. 3.5 acetonitrile. chromatography. 3.6 saturated acetonitrile. acetonitrile and n-hexane were mixed in a separatory funnel and shaken thoroughly. Still stratification, lower solution was collected and dissolved in a liter It was dissolved 100mg of L- ascorbyl palmitate. 3.7 TBHQ standard stock solution. Weigh accurately 100mgTBHQ, Saturated dissolved in acetonitrile and dilute to 100mL. TBHQ concentration 1mg/mL, 0 ℃ ~ 4 ℃ stored. 3.8 TBHQ standard solution. accurate Pipette stock solution 1mLTBHQ, add saturated acetonitrile volume to 100mL, Pipette 1.0, respectively, 5.0,10.0,20.0,50.0mL the TBHQ solution into the 100mL flask, add saturated acetonitrile to the mark, made TBHQ content points Do as TBHQ standard solution 0.1,0.5,1,2,5,10.0μg/mL for the development of a standard curve.

4 instruments and equipment

4.1 high performance liquid chromatography, with a fluorescence detector. 4.2 pipette. 4.3 oscillator. 4.4 Millipore filter. pore size of 0.45μm, organic solvent-based filters. Step 5 Analysis 5.1 Sample Preparation 5.1.1 edible oils and fats Weigh mixed sample of 10g (accurate to 0.001g), with n-hexane was dissolved and set the volume to 100mL. Pipette 20mL of this solution To 150mL separating funnel, adding 20mL saturated acetonitrile, shaking 1min. Still stratification, lower acetonitrile layer was collected, then add 20mL saturated acetonitrile extraction was repeated once, acetonitrile layer was combined volume to 50mL. Through 0.45μm membrane filter as the test solution.

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