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Tobacco casings. Determination of glucose, fructose and sucrose. Ion chromatographic method
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YC/T 252-2008
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Basic data | Standard ID | YC/T 252-2008 (YC/T252-2008) | | Description (Translated English) | Tobacco casings. Determination of glucose, fructose and sucrose. Ion chromatographic method | | Sector / Industry | Tobacco Industry Standard (Recommended) | | Classification of Chinese Standard | X85 | | Classification of International Standard | 65.160 | | Word Count Estimation | 8,856 | | Date of Issue | 2008-04-14 | | Date of Implementation | 2008-04-14 | | Quoted Standard | GB/T 6682; YC/T 145.10 | | Issuing agency(ies) | State Tobacco Monopoly Administration | | Summary | This standard specifies the cigarette liquid glucose, fructose, sucrose ion chromatographic method. This standard applies to cigarette liquid glucose, fructose, sucrose determination. |
YC/T 252-2008: Tobacco casings. Determination of glucose, fructose and sucrose. Ion chromatographic method ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tobacco casings.Determination of glucose, fructose and sucrose.Ion chromatographic method
ICS 65.160
X85
Record Number. 23591-2008
People's Republic of China tobacco industry standards
Determination of solid to liquid glucose tobacco, fructose, sucrose
Ion Chromatography
Posted 2008-04-14
2008-04-14 implementation
State Tobacco Monopoly Bureau issued
Foreword
Appendix A of this standard is an informative annex.
This standard was proposed by the State Tobacco Monopoly Bureau.
This standard by the National Standardization Technical Committee on Tobacco (TC144) centralized.
This standard was drafted. Tobacco Guangdong Industrial Corporation, Research Center for Eco-Environmental Sciences, China Tobacco Hunan Industrial Corporation, Shanghai Tobacco
(Group) Company.
Drafters of this standard. light Shen Lin, Hu Jing, Cai Yaqi, Li Feng, Su Tuo line, Sun Wenliang, intercalation of Zhang Xinying, Shih Wen-Chong, Tang Kun sweet.
Determination of solid to liquid glucose tobacco, fructose, sucrose
Ion Chromatography
1 Scope
This standard specifies the smoke glucose, fructose, sucrose ion chromatographic method for determination of the feed solution used.
This standard applies to tobacco use glucose, fructose, sucrose was measured in the feed solution.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
YC/T 145.10 sampling tobacco flavor
Principle 3
Diluted with water and extracted samples of glucose, fructose, sucrose, after separation by anion exchange, electrochemical detection and analysis.
4 Reagents
4.1 In addition to special requirements, should use analytical reagent. Water should be consistent with GB/T 6682 stipulated a water.
4.2 sodium hydroxide solution, 50% (mass fraction).
4.3 benzoic acid solution, 0.1% (mass fraction).
4.4 mobile phase.
--- Mobile phase A. water;
--- Mobile phase B. sodium hydroxide solution, 50mmol/L. Pipette 2.6mL50% sodium hydroxide solution (4.2) to 1000mL capacity
Volumetric flask, dilute with water to volume;
--- Mobile phase C. sodium hydroxide solution, 250mmol/L. Pipette 13.0mL50% sodium hydroxide solution (4.2) to 1000mL
Volumetric flask, dilute with water to volume.
4.5 single standard stock solution. Weigh 0.1g of glucose, fructose, sucrose, accurate to 0.0001g, 0.1% benzoic acid solution
(4.3) was dissolved, and then were transferred to 100mL volumetric flask, with 0.1% benzoic acid solution (4.3) to the mark, formulated at a concentration of about
1000μg/mL stock solution of a single standard. Stored at 4 ℃ conditions, it should be used within one month.
4.6 mixed standard stock solution. Pipette 10.0mL are glucose, fructose, sucrose single standard stock solution (4.5) in a 100mL container
Flask, with 0.1% benzoic acid solution (4.3) diluted to the mark, formulated as a concentration of about 100μg/mL of glucose, fructose, sucrose,
Mixed standard stock solution.
4.7 Mixed Series Working standard solution. Pipette a certain volume were mixed standard stock solution (4.6) in 50mL volumetric flask, with 0.1% of benzene
Formic acid solution (4.3) diluted to the mark, formulated at concentrations of about 0.1μg/mL, 0.5μg/mL, 1.0μg/mL, 2.0μg/mL,
5.0μg/mL, 10μg/mL, glucose, 20μg/mL of fructose, sucrose mixed working standard solution series.
5 Equipment
5.1 Analytical balance. accurate to 0.1mg.
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