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SN/T 4705-2016 English PDF

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SN/T 4705-2016: Chemicals--Honey bee larval toxicity test, single exposure
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PDF similar to SN/T 4705-2016


Standard similar to SN/T 4705-2016

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Basic data

Standard ID SN/T 4705-2016 (SN/T4705-2016)
Description (Translated English) Chemicals--Honey bee larval toxicity test, single exposure
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard G09
Word Count Estimation 10,176
Date of Issue 2016-12-12
Date of Implementation 2017-07-01
Regulation (derived from) National Quality Inspection (2016) No. 590
Issuing agency(ies) General Administration of Customs

SN/T 4705-2016: Chemicals--Honey bee larval toxicity test, single exposure

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Chemical toxicity test of larvae) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Single exposure toxicity test of chemical bee larvae Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules of GB/T 1.1-2009. This standard and the United Nations Organization for Economic Co-operation and Development (OECD) Chemical Testing Methods No. 237 (2013) "bee larvae single exposure The toxicity test (English version) is consistent with the technical content and is a supplement to the OECD Adult Bee Test Guides 213 and 214, which can be regarded as chemicals. A low-level trial in the overall risk assessment program for bees. This standard has the following structural and editorial changes. --- Removed the introduction; --- Added a chapter on "Scope"; --- Renumber each chapter; --- Removed the reference section. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liu Hanwei, Chen Danchao, Tan Wei, Sun Yun, Li Bing, Chen Weiwei, Li Jinhua. Single exposure toxicity test of chemical bee larvae

1 Scope

This standard specifies the method for single exposure toxicity test of chemical bee larvae. This standard applies to the single exposure toxicity of test chemicals (especially insecticidal ingredients or preparations) to bee larvae.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Dose dose The amount (μg) of the test substance was expressed by the mass of the test substance (μg/larva) used for a single bee larva. 2.2 Half lethal dose LD50 The statistical amount of the test article that caused 50% of the experimental organisms to die after exposure. The half lethal dose of this test is used by a single bee larva The mass of the test substance (μg/larva) was expressed. 2.3 Mortality The proportion of dead subject organisms to the total tested organisms.

3 test preparation

3.1 Test organisms 3.1.1 Source of test organisms A bee larvae (Apismellifera) from three bee colonies, each of which is a repeating group. The bee colony needs to have sufficient The feed supply is healthy and as free as possible from infections and parasites, with detailed source and physiological status records. 3.1.2 Test biosan treatment If the bee colony is sanitized or diseased, the date of treatment and the information about the product used should be indicated in the report. test No sanitation shall be carried out within four weeks before the start. 3.1.3 Acquisition of test organisms On the third day before the test (D-3), the isolation cage (containing an empty honeycomb or a honeycomb with a working bee and an empty nest) will be at least The bee kings of the three bee colonies are isolated in their respective bee colonies (Fig. 1), and the isolation cages are close to the beehives containing the eggs. The queen bee on the second day before the test (D-2) After releasing new eggs, the queen bee should not be kept in the isolation cage for more than 30 hours. The isolation time should be reduced to ensure the body shape between the larvae. Age differences are minimized. The hive containing the bee eggs should be placed in the isolation cage before the first day of the test (D-1).

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