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SN/T 3993-2014: Quarantine protocol for infection with batrachochytrium dendrobatidis
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3993-2014 | English | 209 |
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Quarantine protocol for infection with batrachochytrium dendrobatidis
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SN/T 3993-2014
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PDF similar to SN/T 3993-2014
Basic data | Standard ID | SN/T 3993-2014 (SN/T3993-2014) | | Description (Translated English) | Quarantine protocol for infection with batrachochytrium dendrobatidis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 8,869 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 6682; GB/T 18088 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the clinical diagnosis of poison dart frog chytrid fungus infection, real time polymerase chain reaction detection method. This standard applies to poison dart frog chytrid fungus infection epidemiology, diagnosis, quarantine and m | SN/T 3993-2014: Quarantine protocol for infection with batrachochytrium dendrobatidis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Poison dart frog chytrid fungus infection quarantine technical specifications)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Poison dart frog chytrid fungus infection quarantine Technical Specifications
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is not equivalent part of the World Organisation for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2012 edition) chapter 2.1.1, etc.
The relevant sections.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit-Entry Inspection and Jilin People's Republic of China
Quarantine Bureau.
The main drafters of this standard. Xie star, Peng Xiaoli, Jian-Ping Shi, Liu Tong, Wang Qun force, Chen Shuangya, THE STAFF Xu Tyube, Sun Fuquan,
Wang Jingming.
Poison dart frog chytrid fungus infection quarantine Technical Specifications
1 Scope
This standard specifies the clinical diagnosis of poison dart frog chytrid fungus infection, real time polymerase chain reaction detection method.
This standard applies to poison dart frog chytrid fungus infection epidemiology, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Abbreviations
The following abbreviations apply to this document.
bp. base pairs (basepair)
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
FAM. fluorescent reporter group (Carboxyfluorescein)
MGB. fluorescence quenching group (Minorgroovebinder)
Real-timePCR. real time polymerase chain reaction (real-timepolymerasechainreaction)
Taq. aquatic habitat heat bacteria (Thermusaquaticu)
4 instruments and reagents
4.1 Instrument
4.1.1 refrigerated centrifuge.
4.1.2 -20 ℃ cryogenic refrigerator.
4.1.3 tissue homogenizer.
4.1.4 real-time PCR reaction tube.
4.1.5 real-time PCR instrument.
4.1.6 autoclave.
4.1.7 UV spectrophotometer.
4.2 Reagents
4.2.1 Water should be consistent with GB/T 6682 in a water regulations.
4.2.2 DNA containing poison dart frog chytrid fungus or a plasmid containing the amplified region sequences.
4.2.3 TaqManFastUniversalPCRMasterMix (including dNTP, Taq polymerase and PCR reaction buffer, etc.).
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