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SN/T 3991-2014 English PDF

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SN/T 3991-2014: Detection of akabane virus with real-time RT-PCR
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Standard similar to SN/T 3991-2014

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Basic data

Standard ID SN/T 3991-2014 (SN/T3991-2014)
Description (Translated English) Detection of akabane virus with real-time RT-PCR
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 12,183
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Quoted Standard GB/T 6682; GB 19489
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the Akabane virus real-time fluorescent RT-PCR detection method. This standard applies to livestock and their products in Akabane virus test.

SN/T 3991-2014: Detection of akabane virus with real-time RT-PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Akabane virus real-time fluorescent RT-PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Akabane virus real-time fluorescent RT-PCR detection method Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Ningbo Entry-People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters. Zhang Ji Hong, Tang Tai Shan, Huang Suwen, Rusong, Ni Jianbo, Wang Jianfeng, Zhang often printed. Akabane virus real-time fluorescent RT-PCR detection method

1 Scope

This standard specifies the Akabane virus real-time fluorescent RT-PCR detection method. This standard applies to livestock and their products in Akabane virus test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions adapted to the present document. 3.1.1 Real-time fluorescent RT-PCRrealtimeRT-PCR Join fluorophore in the RT-PCR reaction system, the use of real-time monitoring of the accumulation of the fluorescent signal of the entire RT-PCR amplification process. 3.1.2 Ct values cyclethreshold When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced. 3.2 Acronyms The following abbreviations apply to this document. AKAV. Akabane virus (Akabanevirus) DEPC. diethyl pyrocarbonate (Diethypyrocarbonate) dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate) FAM. 6- carboxyfluorescein (6-carboxyfiuorescein) PBS. phosphate buffer (phosphate-bufferedsaline) RNA. ribonucleic acid (RibonucleicAcid) SDS. sodium dodecyl sulfate (Sodiumdodecylsulfate) TAMRA. 6- carboxy-rhodamine (6-carboxytetramethylrhodamine) Tris. Tris (hydroxymethyl) aminomethane [tris (hydroxymethyl) aminomethane] Taq enzyme. TaqDNA polymerase (TaqDNApolymerase)

4 instruments and equipment

4.1 fluorescence quantitative PCR amplification.

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