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Detection and identification of Narcissus late season yellows virus
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SN/T 3962-2014
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PDF similar to SN/T 3962-2014
Basic data | Standard ID | SN/T 3962-2014 (SN/T3962-2014) | | Description (Translated English) | Detection and identification of Narcissus late season yellows virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,185 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the yellows virus testing procedures and methods Narcissus late season. This standard applies to inbound and outbound detection narcissus Narcissus late season yellows virus. | SN/T 3962-2014: Detection and identification of Narcissus late season yellows virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Quarantine and identification of late season yellow daffodils virus)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of Narcissus late season yellows virus
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine, Fujian Agriculture and Forestry University.
The main drafters of this standard. Shen Jianguo, Liao Furong, Mark dispute, Cai Wei, Li Min, Zhang lights, Yan Cheng, Zhang Yongjiang, Gao Fang Luan, Zu Jian.
Quarantine and identification of Narcissus late season yellows virus
1 Scope
This standard specifies the yellows virus testing procedures and methods Narcissus late season.
This standard applies to inbound and outbound detection narcissus Narcissus late season yellows virus.
Principle 2
Serological and molecular biological characteristics Narcissus late season yellows virus is the main basis of the development of methods for identification of quarantine. Narcissus late season yellows
Taxonomic status of the virus, host range, disease symptoms, geographical distribution, transmission, see Appendix particle morphology, genomic and other A.
3 equipment, appliances, and reagents
3.1 Equipment
High-speed refrigerated centrifuge, electronic balance (1/1000g), PCR instrument, electrophoresis, horizontal electrophoresis tank, -20 ℃ and -80 ℃ low temperature freezer
Ultra-low temperature freezer, gel imaging analysis system, pH meter, a microwave, a magnetic stirrer, constant temperature water bath, autoclaves, clean benches, enzymes
Standard instrument.
3.2 Appliances
Various measurement ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL), PCR reaction tube, RNase-free from
Heart tube (1.5mL), mortar, ELISA plates.
3.3 Reagents
ELISA plate antigen capture enzyme-linked immunosorbent assay (plate-trappedantigenELISA, PTA-ELISA) reagent in Appendix B,
Nested RT-PCR reagents in Appendix C.
4 Detection and Identification
4.1 PTA-ELISA
Specific methods in Appendix B.
4.2 nested RT-PCR
Specific methods, see Appendix C.
5 results found recording
5.1 Result judgment
When yellows virus PTA-ELISA test results were positive Narcissus late season, and nested RT-PCR test results are positive, then the sentence
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