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SN/T 3961-2014 English PDF

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SN/T 3961-2014: Detection and identification of Canna yellow mottle virus
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SN/T 3961-2014English319 Add to Cart 3 days [Need to translate] Detection and identification of Canna yellow mottle virus Valid SN/T 3961-2014

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Basic data

Standard ID SN/T 3961-2014 (SN/T3961-2014)
Description (Translated English) Detection and identification of Canna yellow mottle virus
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 12,118
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies Canna yellow mottle virus detection procedures and methods. This standard applies to the entry and exit of plants and their products Canna yellow mottle virus detection.

SN/T 3961-2014: Detection and identification of Canna yellow mottle virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Quarantine and identification of Canna yellow mottle virus) People's Republic of China Entry-Exit Inspection and Quarantine Standards Quarantine and identification of Canna yellow mottle virus Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine, Fujian Agriculture and Forestry University. The main drafters of this standard. Shen Jianguo, Huang gang water, Chen Jinsong, Li Min, Liao Furong, Zhang Yongjiang, Cai Wei, Yan Cheng, Weng Ruiquan, Zu Jian. Quarantine and identification of Canna yellow mottle virus

1 Scope

This standard specifies Canna yellow mottle virus detection procedures and methods. This standard applies to the entry and exit of plants and their products Canna yellow mottle virus detection. Principle 2 Genomic features Canna yellow mottle virus is the main basis of the quarantine and identification method developed. Canna yellow mottle virus free land Bit, host range, disease symptoms, geographical distribution, transmission, see Appendix particle morphology, genomic and other A. 3 equipment, appliances, and reagents 3.1 Equipment High-speed refrigerated centrifuge, electronic balance (1/1000g), real-time fluorescence PCR, PCR instrument, electrophoresis, horizontal electrophoresis tank, -20 ℃ low temperature -80 ℃ ultra-low temperature refrigerator and freezer gel imaging analysis system, pH meter, a microwave, a magnetic stirrer, water bath pot, Autoclave, Clean bench and so on. 3.2 Appliances Various measurement ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL), PCR reaction tubes, centrifuge tubes (1.5mL), mortar and the like. 3.3 Reagents Nested PCR reagents see Appendix B, real-time PCR reagents in Appendix C.

4 Detection and Identification

4.1 Nested PCR Specific methods in Appendix B. 4.2 real-time PCR Specific methods, see Appendix C.

5 results found recording

5.1 Result judgment --- When the test results nested PCR, real-time PCR two detection methods were positive, it is determined that the detection CaYMV. --- When the test results nested PCR, real-time PCR two detection methods were negative, it is determined not detected CaYMV.

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