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Determination of fat-soluble colorants in food for export
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SN/T 3638-2013
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Basic data Standard ID | SN/T 3638-2013 (SN/T3638-2013) | Description (Translated English) | Determination of fat-soluble colorants in food for export | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | X04 | Classification of International Standard | 67.050 | Word Count Estimation | 41,481 | Quoted Standard | GB/T 6682 | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the food in Sudan Orange G, methyl yellow, Para Red, Red No. 2, citrus, Sudan Red G, Sudan I, Sudan II, Sudan III, Sudan Red 7B, Sudan B, a total of 11 kinds of Sudan IV lipophilic colorants, two methods, i. e., high performance li |
SN/T 3638-2013: Determination of fat-soluble colorants in food for export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of fat-soluble colorants in food for export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of export food liposoluble colorant
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hebei Exit Inspection and Quarantine.
The main drafters of this standard. Wang Fengchi, Li Wei, Mayu Song, Guo Chunhai, Yilian Feng, Duan Wenzhong, Wei Xinxin.
Determination of export food liposoluble colorant
1 Scope
This standard specifies the export of food in Sudan Orange G, methyl yellow, Para Red, Citrus Red No. 2, Sudan G, Sudan Ⅰ, Sudan Ⅱ, Sudan
Ⅲ, Sudan Red 7B, Sudan B, Sudan Ⅳ total of 11 kinds of two methods for determining fat-soluble colorants, namely high performance liquid chromatography (HPLC method)
And liquid chromatography - mass spectrometry/mass spectrometry (LC-MS/MS method).
This standard applies to chili, chili sauce, coated peanuts, sausages and candy Sudan Orange G, methyl yellow, Para Red, Citrus Red No. 2, Sudan
Red G, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ, Sudan Red 7B, Sudan B, measured Sudan Ⅳ11 fat-soluble colorant.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Method 3 first high performance liquid chromatography (HPLC)
3.1 Principle
Using ethyl acetate or acetonitrile - ethyl acetate solution was extracted fat-soluble food colorant, by gel permeation chromatography (GPC), eluted with
Equipped with a diode array detector performance liquid chromatograph, external standard.
3.2 Reagents and materials
Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations.
3.2.1 ethyl acetate.
3.2.2 cyclohexane.
3.2.3 Methanol. HPLC fractions.
3.2.4 acetonitrile. HPLC fractions.
3.2.5 over anhydrous sodium sulfate.
3.2.6 ethyl acetate - acetonitrile (11 volume). Equal volumes of ethyl acetate (3.2.1) and acetonitrile (3.2.4) were mixed uniformly.
3.2.7 ethyl acetate - cyclohexane (11, volume ratio). Equal volumes of ethyl acetate (3.2.1) with cyclohexane (3.2.2) were mixed uniformly.
3.2.8 Standard materials. Sudan Orange G, methyl yellow, Para Red, Citrus Red No. 2, Sudan G, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ, Sudan
7B, Sudan B, Sudan Ⅳ. purity of greater than or equal to 98%, stored in a refrigerator protected from light, for more information see Appendix A.
3.2.9 Standard stock solution. Weigh separately Sudan Orange G, methyl yellow, Para Red, orange red No. 2, Sudan G, Sudan Ⅰ, Sudan Ⅱ,
Sudan Ⅲ, Sudan Red 7B, Sudan B, Sudan Ⅳ standard each 10mg (accurate to 0.1mg), respectively, with ethyl acetate each volume to
100mL, formulated as standard stock solution 100μg/mL of. This solution was below 4 ℃ stored.
3.2.10 intermediate mixed standard solution. accurate Pipette Sudan Orange G, methyl yellow, Para Red, orange red No. 2, Sudan G, Sudan Ⅰ, Sudan Ⅱ,
Sudan Ⅲ, Sudan Red 7B, Sudan B, Sudan Ⅳ standard stock solution of each 1mL to 100mL flask with methanol to volume with
Prepared at a concentration of 1μg/mL, the following 4 ℃ stored.
3.2.11 mixed standard working solution. methanol mixed intermediate standard solution stepwise diluted to 20ng/mL, 50ng/mL according to need,
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