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www.ChineseStandard.net Database: 189760 (25 Oct 2025)

SN/T 1247.2-2003 English PDF

US$359.00 · In stock
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SN/T 1247.2-2003: Protocol of immunoperoxidase monolayer assay for porcine reproductive and respiratory syndrome
Status: Obsolete

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1247.2-2003	English	359	Add to Cart	3 days [Need to translate]	Protocol of immunoperoxidase monolayer assay for porcine reproductive and respiratory syndrome	Obsolete	SN/T 1247.2-2003

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Basic data

Standard ID	SN/T 1247.2-2003 (SN/T1247.2-2003)
Description (Translated English)	Protocol of immunoperoxidase monolayer assay for porcine reproductive and respiratory syndrome
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	B41
Classification of International Standard	11.220
Word Count Estimation	9,983
Date of Issue	2003-05-28
Date of Implementation	2003-12-01
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China

SN/T 1247.2-2003: Protocol of immunoperoxidase monolayer assay for porcine reproductive and respiratory syndrome

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of immunoperoxidase monolayer assay for porcine reproductive and respiratory syndrome Book of the People's Republic of China Entry and Exit Inspection and Quarantine Porcine reproductive and respiratory syndrome immunity Peroxidase monolayer test Released on.2003-05-28 Implementation of.2003-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is based on the World Organisation for Animal Health (OIE) "Diagnostic Tests and Vaccine Standards for Mammalian, Avian and Bee A and B Diseases" Book (Fourth Edition,.2000) Part III X. 12 content is written. Appendix A, Appendix B and Appendix C of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Su Yongsheng, Sun Yingjie, Jian Zhongyou, Sun Yanfeng, Pan Fengcheng. This standard is the industry standard for inspection and quarantine issued for the first time. Immune peroxidase monolayer test for porcine reproductive and respiratory syndrome

1 Scope

This standard specifies the method for the detection of antibodies to porcine reproductive and respiratory syndrome using the immunoperoxidase monolayer test. This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of porcine reproductive and respiratory syndrome.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this standard. 3.1 AEC Aminoethyl carbazole. 3.2 HPRO Horseradish peroxidase. 3.3 IPMA Immunoperoxidase monolayer assay. 3.4 PRRS Porcine reproductive and respiratory syndrome.

4 Inspection and quarantine

4.1 Principle The immunoperoxidase monolayer test is to adsorb an antigen on a solid phase carrier, and add a sample to be tested to form an antigen-antibody complex. The enzyme-labeled anti-antibody is colored with the participation of the substrate, and the result is determined by visual inspection with a naked eye or a spectrophotometer after the substrate is developed. The principle of this standard is to culture cells inoculated with porcine reproductive and respiratory syndrome virus on 96-well cell culture plates. Into a certain proportion of diluted serum to be tested, after the end of the reaction, add anti-porcine immunoglobulin antibody labeled with HPRO, after the reaction is completed, The substrate was added and the reaction results were examined. 4.2 Sampling Collect the blood of the pig to be tested, separate the serum, the serum must be fresh, transparent, non-hemolyzed, non-polluting, and sealed in a sterile vial at 4 ° C or -20 ° C ~ -30 ° C refrigerator storage or cold storage conditions immediately sent for inspection. The serum to be tested was numbered uniformly before the test. 4.3 Equipment and materials 4.3.1 Instrumentation ---Micropipette; ---Heat box; ---Ordinary incubator and carbon dioxide incubator; ---Ordinary refrigerator and low temperature refrigerator; ---Inverted microscope;

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