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GB/T 45538-2025: Determination of plant extracts antioxidant activity - Thin layer chromatography method
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Determination of plant extracts antioxidant activity - Thin layer chromatography method
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GB/T 45538-2025
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Basic data | Standard ID | GB/T 45538-2025 (GB/T45538-2025) | | Description (Translated English) | Determination of plant extracts antioxidant activity - Thin layer chromatography method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B30 | | Classification of International Standard | 65.020.99 | | Word Count Estimation | 10,175 | | Date of Issue | 2025-04-25 | | Date of Implementation | 2025-11-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 45538-2025: Determination of plant extracts antioxidant activity - Thin layer chromatography method
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ICS 65.020.99
CCSB30
National Standard of the People's Republic of China
Determination of antioxidant activity of plant extracts by thin layer chromatography
2025-11-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the China National Institute of Standardization.
This document was drafted by. Jiangnan University, Shenzhen Institute of Standards and Technology, China National Institute of Standardization, Shenzhen Analysis and Testing Association,
Shanxi Agricultural University, Beijing Tongrentang Health Pharmaceutical Co., Ltd., Beijing Academy of Science and Technology Analysis and Testing Institute (Beijing Institute of
Chemical Analysis and Testing Center), Laiyang Inspection and Testing Center, Infinitus (China) Co., Ltd., Yunnan Yunke Special Plant Extraction Laboratory Co., Ltd.
Co., Ltd., Hebei Meiye Siwei Biotechnology Co., Ltd., Guangdong Yifang Pharmaceutical Co., Ltd., Nanjing Zhongke Pharmaceutical Co., Ltd., China Metrology Science Research Institute
Institute, Liheweidao (Qingdao) Food Industry Co., Ltd., Food and Drug Environmental Inspection Institute (Shandong) Group Co., Ltd., Ingel Testing and Certification
Certificate (Shandong) Co., Ltd.
The main drafters of this document are. Chen Yisheng, Zha Shenghua, Zhan Songkun, Zhang Hong, Wang Ke, Ouyang Jing, Xi Xingjun, Bian Ruifang, Shi Xudong, Xu Xueming,
Wang Wei, Tao Hong, Lu Zhi, Wang Feifei, Liu Rui, Luo Wenhui, Lv Weisheng, Zhou Yajie, Feng Peng, Wang Shanshan, Zhang Yongchang, Liu Mingsheng, Xu Mingyu, Sun Xili,
Li Yanhong, Xu Hong, Meng Xia, Wang Yanwei, Luo Bing.
Determination of antioxidant activity of plant extracts by thin layer chromatography
1 Scope
This document describes a method for determining the antioxidant activity of plant extracts by thin layer chromatography.
This document is applicable to the determination of antioxidant activity of extracts of chamomile, plantain, forsythia, bamboo leaves or sophora japonica, and the antioxidant activity of other plant extracts.
Oxidative activity assay was used as a reference.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Antioxidant activity
The ability of antioxidants in plant extracts to scavenge free radicals or oxidants.
4 Principle
Thin layer chromatography separation principle. Since the chromatographic stationary phase has different antioxidant activities in plant extracts (such as phenols, flavonoids
The adsorption capacity of the mobile phase is different. When the mobile phase flows through the stationary phase, adsorption, desorption, re-adsorption and re-desorption will occur continuously.
This allows compounds with different antioxidant activities to be separated from each other.
2,2-Biphenyl-1-picrylhydrazyl (DPPH) impregnation color development principle. DPPH has a dark purple appearance and is evenly applied to a thin layer of color by impregnation.
The free radical scavenger on the stationary phase reacts with DPPH to significantly lighten its color, forming a light yellow on a dark purple background.
Color spots.
The compounds with different antioxidant activities in plant extracts were separated by thin layer chromatography and DPPH immersion was used to analyze the antioxidant activity of the compounds.
Color reaction (the result of the color reaction is quantitatively detected by a thin layer chromatography scanner). The peak area of rutin is used as the reference peak area.
The total peak area of the plant extract was taken as the sample peak area, and the comprehensive peak area of the plant extract was expressed by the ratio of the sample peak area to the control peak area (Ai).
Antioxidant activity.
5 Reagents and Materials
Unless otherwise stated, all reagents used in the analysis were of analytical grade.
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