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GB/T 42113-2022 English PDF

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GB/T 42113-2022: Determination of cyanogenic glycosides in agricultural products - Liquid chromatography with tandem-mass spectrometry method
Status: Valid
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GB/T 42113-2022English279 Add to Cart 3 days [Need to translate] Determination of cyanogenic glycosides in agricultural products - Liquid chromatography with tandem-mass spectrometry method Valid GB/T 42113-2022

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Basic data

Standard ID GB/T 42113-2022 (GB/T42113-2022)
Description (Translated English) Determination of cyanogenic glycosides in agricultural products - Liquid chromatography with tandem-mass spectrometry method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B30
Classification of International Standard 67.050
Word Count Estimation 14,168
Date of Issue 2022-12-30
Date of Implementation 2023-07-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 42113-2022: Determination of cyanogenic glycosides in agricultural products - Liquid chromatography with tandem-mass spectrometry method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.050 CCSB30 National Standards of People's Republic of China Determination of Cyanoglycosides in Agricultural Products Liquid Chromatography-Tandem Mass Spectrometry Posted on 2022-12-30 2023-07-01 implementation State Administration for Market Regulation Released by the National Standardization Management Committee

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed and managed by China National Institute of Standardization. This document is drafted by. Institute of Tropical Crop Variety Resources, Chinese Academy of Tropical Agricultural Sciences, Zhejiang University, China National Institute of Standardization, Beijing Beijing Forestry University. The main drafters of this document. Lu Baiyi, Zhang Zhenwen, Yang Li, Chang Zhihui, Wu Xiaodan, Wang Qinfei, Li Kaimian, Zhong Yongheng, Lin Liming, Yu Houmei, Yao Qingqun. Determination of Cyanoglycosides in Agricultural Products Liquid Chromatography-Tandem Mass Spectrometry

1 Scope

This document describes the contents of linamarin, β-gentiobiose acetone cyanohydrin, lotinoside, β-gentiobiose methyl ethyl ketone cyanohydrin and taxane in agricultural products Determination method of liquid chromatography-tandem mass spectrometry for glucoside, teosin, amygdalin and pruninin. This document is applicable to the determination of cyanogenic glycosides in cassava, linseed, sorghum, bamboo shoots, almonds and other agricultural products.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and Definitions

This document does not have terms and definitions that need to be defined.

4 principles

The cyanogenic glycosides in the sample were extracted by 80% methanol aqueous solution, purified by solid phase extraction column, determined by liquid chromatography-tandem mass spectrometry, and external standard method Quantitative.

5 Reagents and Materials

Unless otherwise specified, only analytical reagents and grade-1 water in accordance with GB/T 6682 are used in the analysis. 5.1 Reagents 5.1.1 Methanol (CH3OH, CAS No.. 67-56-1). chromatographically pure. 5.1.2 Acetonitrile (CH3CN, CAS No.. 75-05-8). chromatographically pure. 5.1.3 Formic acid (HCOOH, CAS No.. 64-18-6). chromatographically pure. 5.2 Reagent preparation 5.2.1 0.1% formic acid aqueous solution (volume fraction). accurately absorb 1.00mL of formic acid (5.1.3), and dilute to 1L with water. 5.2.2 80% methanol aqueous solution (volume fraction). Measure 80mL of methanol (5.1.1), add 20mL of water, and mix well. 5.2.3 10% methanol aqueous solution (volume fraction). Measure 10mL of methanol (5.1.1), add 90mL of water, and mix well. 5.2.4 30% methanol-acetonitrile solution (volume fraction). Measure 30mL of methanol (5.1.1), add 70mL of acetonitrile (5.1.2), and mix well.

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