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GB/T 34801-2017: Determination of the activity of desoxyribonuclease
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Determination of the activity of desoxyribonuclease
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GB/T 34801-2017
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Basic data | Standard ID | GB/T 34801-2017 (GB/T34801-2017) | | Description (Translated English) | Determination of the activity of desoxyribonuclease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Classification of International Standard | 07.080 | | Word Count Estimation | 5,575 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34801-2017: Determination of the activity of desoxyribonuclease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of desoxyribonuclease
ICS 07.080
A21
National Standards of People's Republic of China
Deoxyribonuclease activity assay
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the China National Institute of Standardization and centralized.
This standard was drafted unit. China National Institute of Standardization, Georgia Gelin Biotechnology Co., Ltd., Fujian SAFU Food Testing Institute have
Co., Ltd., Zhejiang Gongshang University, Hebei Agricultural University, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Beijing Food Science Research
Institute, Hebei Institute of Food Inspection, Dongying City standardized information, Henan University.
The main drafters of this standard. Ma Aijin, Chen Zhiyong, Huang Xiujuan, Wang Yanbo, Sun Records, Zheng Lei, Liu Daoliang, Sun Yong, Yun Zhenyu, Fu Linglin,
Wu Qi, Zhao Lin, Zhou Wei, Liu Peng, Kang Arts, Zong Xuehua.
Deoxyribonuclease activity assay
1 Scope
This standard specifies the principle of DNAzyme activity testing, equipment and apparatus, the main reagents, analysis steps and results analysis.
This standard applies to biochemical reagents deoxyribonuclease activity test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNase deoxyribonuclease; DNase
Phosphodiester hydrolysis in deoxyribonucleic acid (DNA), generating oligonucleotides or mononucleotide nucleases.
3.2
Deoxyribonuclease activity unit ribonucleaseactivityunit
Using 1.5 mL of calf thymus DNA having a concentration of 0.15 mg/mL as a substrate, the cells were cultured at 35 ° C. and pH 5.0 for 1 min
The amount of enzyme required for the absorbance of the reaction solution to increase by 0.001 at 260 nm.
Note. An enzyme activity unit to U said.
4 principle
Deoxyribonuclease catalyzed deoxyribonucleic acid decomposition, the degradation product has a characteristic absorption peak at 260nm wavelength, by external standard method
Deoxyribonuclease activity was calculated.
5 equipment and appliances
5.1 constant temperature water bath. Accuracy of 0.1 ℃.
5.2 UV - visible spectrophotometer Absorbance accurate to 0.001.
5.3 pH meter. Accuracy of 0.01.
5.4 1cm quartz cuvette.
5.5 electronic balance with accuracy of 0.0001g, 0.01g or 0.1g.
6 major reagents
Reagents used in this method are of analytical grade, except for special instructions, the experimental water are secondary water GB/T 6882 regulations.
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