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GB/T 34800-2017: Detection method of activity and impurity of proteinase K
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| GB/T 34800-2017 | English | 119 |
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Detection method of activity and impurity of proteinase K
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GB/T 34800-2017
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Basic data | Standard ID | GB/T 34800-2017 (GB/T34800-2017) | | Description (Translated English) | Detection method of activity and impurity of proteinase K | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,668 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34800-2017: Detection method of activity and impurity of proteinase K---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method of activity and impurity of proteinase K
ICS 07.080
A21
National Standards of People's Republic of China
Proteinase K enzyme activity and impurity detection methods
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the China National Institute of Standardization and centralized.
This standard was drafted. Dongying Ruida Biological Technology Co., Ltd., China National Institute of Standardization, China Biotechnology Development Center, Hebei Agricultural
Industry University, Zhejiang Gongshang University, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Quanzhou Institute of Standardization, Beijing Food
Institute of Science, Hebei Institute of Food Inspection, Henan University.
The main drafters of this standard. Fang Cunlin, Ma Ai Jin, Su Yue, Sun Records, Wang Yanbo, Zheng Lei, Liu Daoliang, Lin Qingshan, Sun Yong, Yun Zhenyu,
Fu Linglin, Zhao Lin, Wu Qi, Zhou Wei, Kang Arts, Chen Xinkai.
Proteinase K enzyme activity and impurity detection methods
1 Scope
This standard specifies the protease K detection principle, equipment and apparatus, the main reagents, analysis steps and results analysis.
This standard applies to biochemical reagents Proteinase K product production and testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 34222 ribonuclease activity test method
GB/T 34801 DNAzyme activity assay
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Protease K proteinaseK
A serine protease that hydrolyzes native keratin.
Note. By product form is divided into solid and liquid.
3.2
Protease K enzyme activity unit proteinaseKactivityunit
Hydrolysis of bovine hemoglobin within 1 min at 57 ° C and pH 8.0 yielded an enzyme amount of 1 μmol of phenolic amino acids.
Note. An enzyme activity unit to U said.
4 principle
Proteinase K catalyzes the cleavage of the carboxyl-terminal peptide bonds of aliphatic and aromatic amino acids. It is at 57 ° C and pH8.0 conditions,
Hydrolysis of bovine hemoglobin substrate produces phenolic-containing amino acids; under basic conditions, Folin reagent can be reduced to form molybdenum blue and tungsten
Blue, the color depth is proportional to the content of phenolic amino acids. By measuring the absorbance at 680 nm, a phenylamino group produced by hydrolysis is obtained
The amount of acid, and then calculate the activity of protease K enzyme.
5 equipment and appliances
5.1 constant temperature water bath. Accuracy of ± 0.1 ℃.
5.2 pH meter. Accuracy of 0.1pH units.
5.3 spectrophotometer wavelength range 380nm ~ 780nm, the absorbance value accurate to 0.001.
5.4 1cm cuvette.
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