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GB/T 28103-2011 English PDF

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GB/T 28103-2011: Detection and identification of wheat streak mosaic virus
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GB/T 28103-2011English229 Add to Cart 3 days [Need to translate] Detection and identification of wheat streak mosaic virus Valid GB/T 28103-2011

PDF similar to GB/T 28103-2011


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Basic data

Standard ID GB/T 28103-2011 (GB/T28103-2011)
Description (Translated English) Detection and identification of wheat streak mosaic virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 10,124
Date of Issue 2011-12-30
Date of Implementation 2012-06-01
Quoted Standard SN/T 1840; SN/T 2122
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the wheat streak mosaic virus (wheat streak mosaic virus) quarantine and identification. This standard applies to grasses seeds and seedlings of wheat streak mosaic virus quarantine and identification.

GB/T 28103-2011: Detection and identification of wheat streak mosaic virus

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Detection and identification of wheat streak mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Wheat streak mosaic virus quarantine and identification methods Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Guo Jingze, Liu Fang, Liuyue Ting, Zhang Yujun, Liu Yong, Cui Tiejun, Wang Jincheng. Wheat streak mosaic virus quarantine and identification methods

1 Scope

This standard specifies the wheat streak mosaic virus (wheatstreakmosaicvirus) quarantine and identification methods. This standard applies to seed and seedling grasses in wheat streak mosaic virus quarantine identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 Wheat streak mosaic virus Basic Information

Wheat streak mosaic virus wheatstreakmosaicvirus, belonging to potato virus Y Branch Potyviridae, wheat mosaic virus genus Tritimovirus. Abbreviation. WSMV. Wheat streak mosaic virus spread through. juice friction propagation; seed-borne; mediator spread. The virus is easy to close by frictional diffusion juice. The virus can be sick wheat seeds transported over long distances. Disease Toxic mass poisoning mediator wheat curl mite Aceriatosichella, adults and nymphs can be carriers of the virus infection, the virus does not pass the eggs. Wheat streak mosaic virus For additional information, see Appendix A.

4 principle of the method

Morphological characteristics of wheat streak mosaic virus, molecular biological characteristics and serologic characteristics of the virus by a major quarantine and identification methods according to. Double antibody sandwich enzyme-linked immunosorbent assay methods, plant viral immune electron microscopy method, reverse transcription-polymerase chain reaction Methods to determine the wheat streak mosaic virus samples with the. 5 equipment, appliances, and reagents 5.1 Equipment Reader, washer, heated water bath, ordinary refrigerator (0 ℃ ~ 4 ℃), - 20 ℃ low temperature freezer, -80 ℃ ultra-low temperature freezer, plant Light incubator (with adjustable temperature range of 0 ℃ ~ 50 ℃), clean bench, electronic balance (1/10000g), trace juicer, transmission electron Microscope, low-speed centrifuge (speed of 3000r/min ~ 5000r/min), PCR instrument, water meter, electrophoresis, gel imaging system. 5.2 Appliances Adjustable micropipette (2.5μL, 10μL, 100μL, 1000μL, 5000μL) and the corresponding tip, ELISA plate, sealing membrane, pH Meter or pH test strip (wide strip and precision strip), 1.5mL centrifuge tubes, PCR reaction tube, flask (500mL, 1000mL), solution Sectional blade, tweezers, scissors, absorbent paper, white porcelain plate, markers, labels, disposable gloves.

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