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GB/T 28094-2011 English PDF

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GB/T 28094-2011: Detection and identification of Xanthomonas campestris pv. mangiferaeindicae (Patel et al.) Robbs et al.
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 28094-2011	English	229	Add to Cart	3 days [Need to translate]	Detection and identification of Xanthomonas campestris pv. mangiferaeindicae (Patel et al.) Robbs et al.	Valid	GB/T 28094-2011

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Basic data

Standard ID	GB/T 28094-2011 (GB/T28094-2011)
Description (Translated English)	Detection and identification of Xanthomonas campestris pv. mangiferaeindicae (Patel et al.) Robbs et al.
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B16
Classification of International Standard	65.020.01
Word Count Estimation	10,136
Date of Issue	2011-12-30
Date of Implementation	2012-06-01
Quoted Standard	SN/T 1193; SN/T 2122
Regulation (derived from)	Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary	This standard specifies the mango bacterial black spot pathogen of quarantine and identification. This standard applies to mango fruit, mango seedlings and other plant material bacterial Alternaria quarantine and identification.

GB/T 28094-2011: Detection and identification of Xanthomonas campestris pv. mangiferaeindicae (Patel et al.) Robbs et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas campestris pv. Mangiferaeindicae (Patel et al.) Robbs et al. ICS 65.020.01 B16 National Standards of People's Republic of China Mango bacterial black spot disease quarantine and identification methods mangiferaeindicae (Pateletal.) Robbsetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Liu Peng, Feng Roca, Cuiru Jiang, Wei Yadong, Wang Jincheng, Liu Yong, Zhao Lirong, Huang Guoming. Mango bacterial black spot disease quarantine and identification methods

1 Scope

This standard specifies the method for inspection and identification of mango bacterial black spot pathogen. This standard applies to quarantine and identification of mango fruits, seedlings and other plant material mango bacterial black spot pathogen.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1193 genetic testing laboratory technical requirements SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 mango bacterial black spot pathogen basic information

Chinese name. mango bacterial black spot pathogen. Scientific name. Xanthomonascampestrispv.mangiferaeindicae (Patel, Moniz Ribeiro Disease English. mangobacterialblackspot. Spp community Bacteria, Proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Xanthomonas Head Xanthomonodales, Xanthomonas Branch Xanthomonodaceae, Xanthomonas Xanthomonas. Germs lurking in the diseased leaves, sick branches, fruit and other organizations wintering disease is the main source of primary infection. Germs by wind and rain, and water contact, Long-distance spread is mainly contaminated seedlings, scion and fruits and so on. For additional information, see Appendix mango bacterial black spot pathogen A.

4 principle of the method

Based on characteristic symptoms, and biological characteristics of the pathogen, physiological and biochemical characteristics, molecular characterization and pathogenicity characteristics were seized Test identification.

5 Apparatus appliances

Biological microscope, clean benches, autoclave, incubator, centrifuge (12000r/min), a spectrophotometer, PCR instrument, Electrophoresis, gel imaging, electronic balance (inductance 0.0001g), heated water bath, constant light incubator, low temperature refrigerators, micro adjustable plus Injector (10μL, 100μL, 200μL, 1000μL) and the like.

6 Reagents and culture medium

6.1 Reagents MgCl2, dNTPs (dATP, dTTP, dCTP, dGTP), Taq enzyme, oxidase test strip, biochemical identification of bacteria micro tube, primer

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