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GB/T 23187-2008 English PDF

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GB/T 23187-2008: Determination of lutein in feeds -- High performance liquid chromatography
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PDF similar to GB/T 23187-2008


Standard similar to GB/T 23187-2008

GB 22549   GB 22548   GB/T 23742   GB/T 23186   GB/T 23179   

Basic data

Standard ID GB/T 23187-2008 (GB/T23187-2008)
Description (Translated English) Determination of lutein in feeds -- High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B46
Classification of International Standard 65.120
Word Count Estimation 5,583
Date of Issue 2008-12-31
Date of Implementation 2009-05-01
Quoted Standard GB/T 6682; GB/T 14699.1; GB/T 20195
Regulation (derived from) National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method of high performance liquid chromatography feed lutein. This standard applies to single feed, additive premix feed, concentrated feed and feed with the determination of lutein. Quantitation limit is 0. 5mg/kg.

GB/T 23187-2008: Determination of lutein in feeds -- High performance liquid chromatography

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Determination of lutein in feeds. High performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China Dietary lutein by HPLC Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points. This standard was drafted. Huazhong Agricultural University, Guangzhou-standard detection technology center, Guangzhou Li Dahl Biotechnology Co., Ltd., Foshan City, South Biotechnology Co., Ltd. Sea Vader. The main drafters of this standard. OILS, inflammation in the lake, Chen, Tao Zhengguo, Gao Qin, Kuang-mei, Gu Chui Peng, Huang Kai Bin, Sun Yuguo, Zhang Antonia. Dietary lutein by HPLC

1 Scope

This standard specifies the method of high performance liquid chromatography in feed lutein. This standard applies to single feed, feed additive premix, concentrated feed and feed with the determination of lutein. The limit of quantification Of 0.5mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT) GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT) Principle 3 Lutein sample after alkaline saponification and solvent extraction, the supernatant was filtered, high performance liquid chromatography separation with purple External detector and calculate the content of lutein external standard method.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade reagent water in line with GB/T 6682 stipulated a water. 4.1 n-hexane. 4.2 acetone. 4.3 ethanol. 4.4 toluene. 4.5 methanol. 4.6 isopropanol. 4.7 potassium hydroxide. 4.8 over anhydrous sodium sulfate. 4.9 Extraction solvent. n-hexane - acetone - ethanol - toluene (34 + 23 + 20 + 23) mixture. 4.10 methanol solution of potassium hydroxide (400g/L). 40g of potassium hydroxide dissolved in methanol, cooled and diluted to 100mL with methanol. 4.11 sodium sulphate solution (100g/L). 10g of anhydrous sodium sulfate were dissolved in 100mL water. 4.12 Lutein standard stock solution. Weigh accurately lutein standard (content greater than 99.0%) 2mg (accurate to 0.1mg), to join the less The amount of mobile phase sonication, with the mobile phase constant volume to 100mL brown volumetric flask, as the standard stock solution, 4 ℃ dark preservation, storage period of 3 days. 4.13 Lutein standard working solution. take lutein stock standard solution (4.12) with the mobile phase progressively diluted 0.5μg/mL, 1.0μg/mL, 2.0μg/mL, 5.0μg/mL, 10.0μg/mL, 20.0μg/mL series of standard working solution.

5 Instruments

5.1 constant temperature water bath. 5.2 Analysis of balance. a sense of the amount of 0.0001g. 5.3 High performance liquid chromatograph. with UV-VIS detector.

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