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GB/T 22506-2008: Inspection of grain and oils -- Determination of 1- and 2-acyl lysophosphatidylcholine in enzymatically modified phospholipids -- High performance liquid chromatography
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| GB/T 22506-2008 | English | 139 |
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Inspection of grain and oils -- Determination of 1- and 2-acyl lysophosphatidylcholine in enzymatically modified phospholipids -- High performance liquid chromatography
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GB/T 22506-2008
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Basic data | Standard ID | GB/T 22506-2008 (GB/T22506-2008) | | Description (Translated English) | Inspection of grain and oils -- Determination of 1- and 2-acyl lysophosphatidylcholine in enzymatically modified phospholipids -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.040 | | Word Count Estimation | 6,639 | | Date of Issue | 2008-11-04 | | Date of Implementation | 2009-01-20 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.18 (Total No.131) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the determination of enzyme modified lecithin 1 by high performance liquid chromatography-Principle of lysophosphatidylcholine-lysophosphatidylcholine and 2, reagents, equipment, sample preparation, procedure, Results are expressed and precision and accuracy. This standard applies to soy lecithin, yolk phospholipids, measurement was prepared from rapeseed phospholipid phosphatidylcholine hemolysis. The detection limit of the standard 0. 1��g. | GB/T 22506-2008: Inspection of grain and oils -- Determination of 1- and 2-acyl lysophosphatidylcholine in enzymatically modified phospholipids -- High performance liquid chromatography
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Inspection of grain and oils. Determination of 1- and 2- acyl lysophosphatidylcholine in enzymatically modified phospholipids. High performance liquid chromatography
ICS 67.040
X04
National Standards of People's Republic of China
Inspection of grain in the 1- and enzyme modified lecithin
Determination of 2- lysophosphatidylcholine
High Performance Liquid Chromatography
Posted 2008-11-04
2009-01-20 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
The standard proposed by the National Food Authority.
This standard by the National Standardization Technical Committee OILS.
This standard was drafted. Jiangnan University.
The main drafters of this standard. WangXingGuo, Jin Qingzhe, Shan Liang, Liu Yuan Law, PROJECTILES, Pan Qiuqin.
Inspection of grain in the 1- and enzyme modified lecithin
Determination of 2- lysophosphatidylcholine
High Performance Liquid Chromatography
1 Scope
This standard specifies the determination of enzyme modified lecithin 1-lysophosphatidylcholine (1-aclylysophosphatidyl- by high performance liquid chromatography
choline; 1-LPC) and 2-lysophosphatidylcholine (2-aclylysophosphatidylcholine; 2-LPC) in principle, reagents, instruments provided
Equipment, sample preparation, procedures, and results indicate precision and accuracy.
This standard applies to soy lecithin, egg yolk lecithin, rapeseed lecithin as the raw material for preparing the determination of the lysophosphatidylcholine.
The standard detection limit of 0.1μg.
2 Normative references
Following standard terms of this standard reference and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Enzyme modified lecithin products in the 1- and 2-lysophosphatidylcholine and phosphatidylcholine (PC) and other components separated by high performance liquid chromatography,
Using differential refractive index detector and quantified by external standard.
4 Reagents
The following reagents used, unless otherwise specified, are analytical reagents; water is in line with GB/T 6682 provided a water.
4.1 95% ethanol.
4.2 oxalate (H2C2O4 · 2H2O).
4.3 oxalic acid solution (0.02moL/L). Weigh accurately 1.261g of oxalic acid (4.2), dissolved in water and diluted to 500mL.
4.4 1 Phospholipid standard). phosphatidylcholine (PC), 1- palmitoyl - lysophosphatidylcholine (1-LPC), 2- palmitoyl - lysophosphatidylcholine
(2-LPC).
1) standard can be used Sigma's products. Sigma-Aldrich is given only for the convenience of users of this standard, no other commercial activity, other who can
Enough to reach the required standard, you can choose to use.
5 Equipment
5.1 High performance liquid chromatograph. with refractive index detector.
5.2 high-speed centrifuge. 10000r/min.
5.3 fiber membrane. 0.45μm.
5.4 ultrasonic cleaning. 180W.
5.5 Balance. dividing the value of 0.0001g.
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