Search result: GB/T 22246-2025 (GB/T 22246-2008 Older version)
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Determination of pantothenic acid in health foods
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GB/T 22246-2025
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Determination of calcium pantothenate in health foods
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GB/T 22246-2008
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| Standard ID | GB/T 22246-2025 (GB/T22246-2025) | | Description (Translated English) | Determination of pantothenic acid in health foods | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X83 | | Classification of International Standard | 67.050 | | Word Count Estimation | 10,117 | | Date of Issue | 2025-01-24 | | Date of Implementation | 2025-08-01 | | Issuing agency(ies) | State Administration for Market Regulation, National Standardization Administration |
GB/T 22246-2025.Determination of pantothenic acid in health foods
ICS 67.050
CCSX83
National Standard of the People's Republic of China
Replaces GB/T 22246-2008
Determination of Pantothenic Acid in Health Food
Released on 2025-01-24
2025-08-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document specifies the technical requirements related to food quality. For food safety requirements, please refer to relevant laws, regulations, policies and food safety standards.
document.
This document replaces GB/T 22246-2008 "Determination of Calcium Pantothenate in Health Foods". Compared with GB/T 22246-2008, except for the structure
In addition to adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2008 edition);
b) The sample preparation and treatment methods have been changed (see 7.1 and 7.2, 5.1 of the.2008 edition);
c) The calculation formula has been changed (see Chapter 8, Chapter 6 of the.2008 edition);
d) The detection limit and quantification limit have been changed (see Chapter 10, Chapter 1 of the.2008 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Technical Committee for Standardization of Special Foods (SAC/TC466).
This document was drafted by. Jiangsu Aland Nutrition Products Co., Ltd., Jiangzhong Pharmaceutical Co., Ltd., and China Center for Disease Control and Prevention.
Institute of Nursing and Health, Shanghai Quality Supervision and Inspection Technology Research Institute, Jiangsu Huashan Health Technology Co., Ltd., China Light Industry Technology Innovation Center Co., Ltd.
Co., Ltd., Biostime (China) Co., Ltd., China Food Fermentation Industries Research Institute Co., Ltd., and Dongpeng Beverage (Group) Co., Ltd.
The main drafters of this document are. Zhang Xuesong, Chen Fang, Xu Xiaoming, Zheng Guojian, Chi Huazhong, Zhong Qiding, Li Yong, Wang Zhu, Wu Zhuying, Hu Hai'e,
An Hongmei, Wang Daobing, Xu Haoran, Wu Yifan, Yue Hongwei, Wu Xiangqian, Zhang Peixia, Zhu Wei, Zhong Shunhao, Li Xueli, Sheng Jie.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2008 as GB/T 22246-2008;
---This is the first revision.
Determination of Pantothenic Acid in Health Food
1 Scope
This document describes a method for the determination of pantothenic acid in health foods by HPLC.
This document applies to pantothenic acid in health foods in dosage forms such as tablets, hard capsules, soft capsules, granules, powders, oral liquids, and gel candies.
Determination of.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
The pantothenic acid in the sample was extracted with hot water, separated by high performance liquid chromatography and detected by ultraviolet detector. The retention time was used for qualitative analysis and the external standard method was used for quantitative analysis.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Reagents
5.1.1 Water. Grade 1 water as specified in GB/T 6682.
5.1.2 Hydrochloric acid (HCl).
5.1.3 Sodium hydroxide (NaOH).
5.1.4 Phosphoric acid (H3PO4).
5.1.5 Zinc sulfate heptahydrate (ZnSO4·7H2O).
5.1.6 Potassium dihydrogen phosphate (KH2PO4).
5.1.7 Acetonitrile (CH3CN). chromatographic grade.
5.1.8 0.1 mol/L hydrochloric acid solution. Pipette 9 mL of hydrochloric acid (5.1.2) into 800 mL of water, dilute to 1000 mL with water, and mix well.
5.1.9 1.0 mol/L hydrochloric acid solution. Pipette 9 mL of hydrochloric acid (5.1.2) into 80 mL of water, dilute to 100 mL with water, and mix well.
5.1.10 Sodium hydroxide solution (0.01 mol/L). weigh 0.4 g of sodium hydroxide (5.1.3), dissolve it in water and dilute to 1000 mL.
5.1.11 Sodium hydroxide solution (0.1 mol/L). weigh 0.4 g of sodium hydroxide (5.1.3), dissolve it in water and dilute to 100 mL.
5.1.12 Zinc sulfate solution (0.5 mol/L). weigh 14.4 g zinc sulfate heptahydrate (5.1.5), dissolve it in water and dilute to 100 mL.
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GB/T 22246-2008
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.040
C 53
Determination of Calcium Pantothenate in
Health Foods
ISSUED ON. JULY 31, 2008
IMPLEMENTED ON. NOVEMBER 1, 2008
Issued by. Ministry of Health of PRC; Standardization Administration of
China.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Principles ... 4
3 Reagents and Materials ... 4
4 Apparatuses and Instruments ... 5
5 Analysis Steps ... 5
6 Calculation ... 6
7 Precision ... 7
8 Chromatograms ... 7
Foreword
This Standard was proposed by and shall be under the jurisdiction of Ministry of
Health of PRC.
Drafting organizations of this Standard. Chinese Center for Disease Control and
Prevention nutrition and food security.
Participating drafting organizations of this Standard. Hebei Provincial Center for
Disease Control and Prevention.
Chief drafting staffs of this Standard. Yang Dajin, Fang Congrong, Ma Lan, Wang
Zhutian, and Qin Zhenshun.
This Standard is first-time published.
Determination of Calcium Pantothenate in
Health Foods
1 Scope
This Standard specifies the determination method of calcium pantothenate in the
nutrient supplements types of health foods.
This Standard is applicable to determination of calcium pantothenate in the nutrient
supplements types of health foods.
This Standard’s Limit of Detection. when sampling amount is 1.0g, fix the volume to
25mL; when injection amount is 10μL, the Limit of Detection (LOD) is 2x10-4g/100g,
and the Limit of Quantity (LOQ) is 6x10-4g/100g.
This Standard’s linear range. 2.5μg/mL ~ 80μg/mL.
2 Principles
According to calcium pantothenate’s physiochemical properties - it is soluble in water,
it is unstable in acid and alkali, and it is very stable in neutral (pH=5.0~7.0) conditions -
the calcium pantothenate in the specimen shall use water to extract under the
ultrasonic oscillation; after fixed-volume and centrifugation, take supernatant filtration
membrane; go through the separation of C18 reverse phase column; then detect at
200nm wave length of UV detector; identify the qualitative as per the chromatographic
retention time; identify the quantification as per the external-standard method.
3 Reagents and Materials
3.1 Acetonitrile (CH3CN). chromatographic pure.
3.2 Phosphoric acid (H3PO4). analytical reagent.
3.3 Potassium dihydrogen phosphate (KH2PO4). analytical reagent.
3.4 0.02mol/L potassium dihydrogen phosphate solution. take 2.722g of
potassium dihydrogen phosphate; add water to dissolve into 1000mL; use
phosphoric acid to adjust the pH value to 3.0.
3.5 Water (H2O). Grade-1 lab water, conductivity (25°C) is 0.01mS/m.
3.6 Calcium pantothenate standard stock solution. weigh 0.1g of calcium
pantothenate standard product (accurate to 0.0001g); place it in a 100mL flask; add
water to dissolve; fix volume to graduation; mix evenly (keep in 4°C refrigerator for
spare use; such solution can be stored for 5d).
3.7 Calcium pantothenate standard use solution. exactly weigh 1.0mL of standard
stock solution (see Clause 3.6); place it in a 100mL flask; add water to dilute to
graduation; mix evenly (keep in 4°C refrigerator for spare use; such solution can be
stored for 5d).
4 Apparatuses and Instruments
4.1 High performance liquid chromatography instrument. attached with UV Detector.
4.2 Ultrasonic cleaner.
4.3 Centrifuge. 4000r/min.
5 Analysis Steps
5.1 Specimen preparation
5.1.1 Solid tablet or capsule specimens. weigh 0.1g~1.0g of post-pulverizing and
uniform specimen (accurate to 0.001g); make the specimen concentration to be within
the standard curve. Place the specimen into a 25mL flask; add 200mL of water; after
conducting ultrasonic extraction for 20min, add water to the graduation; shake
uniformly; after centrifugation, pass the supernatant through the 0.45μm membrane;
the filtrate is to be injected.
5.1.2 Liquid specimen. except that, for carbonated drink, it needs ultrasound to
remove the carbon dioxide before sampling - for other liquid samples, weigh directly
and accurately the appropriate amount of sample-liquid; make the specimen
concentration to be within the standard curve. Place the specimen into the 25mL flask;
use water to dilute to the graduation, and then mix evenly; pass the specimen through
0.45μm membrane, and set aside filtrate.
5.2 Preparation of standard curve
Absorb 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 mL of calcium pantothenate standard use
solution (equivalent to 2.5μg, 5.0μg, 10μg, 20μg, 40μg, and 80μg of calcium
pantothenate), place them into 10mL flasks respectively, dilute with water to the
graduation, and mix evenly. In the given instrument conditions, conduct
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