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GB/T 22246-2008 (GBT 22246-2008)

Chinese standards (related to): 'GB/T 22246-2008'
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GB/T 22246-2008English85 Add to Cart 0-9 seconds. Auto delivery. Determination of calcium pantothenate in health foods GB/T 22246-2008 Valid GBT 22246-2008


   
BASIC DATA
Standard ID GB/T 22246-2008 (GB/T22246-2008)
Description (Translated English) Determination of calcium pantothenate in health foods
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 67.040
Word Count Estimation 6,650
Date of Issue 2008-07-31
Date of Implementation 2008-11-01
Drafting Organization Chinese Center for Disease Control Top Anti Nutrition and Food Safety
Administrative Organization Ministry of Health
Regulation (derived from) National Standard Approval Announcement 2008 No.12 (Total No.125)
Proposing organization Ministry of Health of the People Republic of China
Issuing agency(ies) The People's Republic of China Ministry of Health, China National Standardization Management Committee
Summary This standard specifies the determination of nutrient supplements in health food calcium pantothenate. This standard applies to the determination of nutrient supplements in health food calcium pantothenate. This standard detection limit: When the sampling volume 1. 0g, set the volume to 25mL, injection volume 10��L, the detection limit of 2 �� 10-4g/100g, quantitation limit of 6 �� 10-4g/100g. This standard linear range: 2. 5��g/mL ~ 80��g/mL.


GB/T 22246-2008: PDF in English (GBT 22246-2008)
GB/T 22246-2008
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.040
C 53
Determination of Calcium Pantothenate in
Health Foods
ISSUED ON. JULY 31, 2008
IMPLEMENTED ON. NOVEMBER 1, 2008
Issued by. Ministry of Health of PRC; Standardization Administration of
China.
Table of Contents
Foreword ... 3 
1 Scope ... 4 
2 Principles ... 4 
3 Reagents and Materials ... 4 
4 Apparatuses and Instruments ... 5 
5 Analysis Steps ... 5 
6 Calculation ... 6 
7 Precision ... 7 
8 Chromatograms ... 7 
Foreword
This Standard was proposed by and shall be under the jurisdiction of Ministry of
Health of PRC.
Drafting organizations of this Standard. Chinese Center for Disease Control and
Prevention nutrition and food security.
Participating drafting organizations of this Standard. Hebei Provincial Center for
Disease Control and Prevention.
Chief drafting staffs of this Standard. Yang Dajin, Fang Congrong, Ma Lan, Wang
Zhutian, and Qin Zhenshun.
This Standard is first-time published.
Determination of Calcium Pantothenate in
Health Foods
1 Scope
This Standard specifies the determination method of calcium pantothenate in the
nutrient supplements types of health foods.
This Standard is applicable to determination of calcium pantothenate in the nutrient
supplements types of health foods.
This Standard’s Limit of Detection. when sampling amount is 1.0g, fix the volume to
25mL; when injection amount is 10μL, the Limit of Detection (LOD) is 2x10-4g/100g,
and the Limit of Quantity (LOQ) is 6x10-4g/100g.
This Standard’s linear range. 2.5μg/mL ~ 80μg/mL.
2 Principles
According to calcium pantothenate’s physiochemical properties - it is soluble in water,
it is unstable in acid and alkali, and it is very stable in neutral (pH=5.0~7.0) conditions -
the calcium pantothenate in the specimen shall use water to extract under the
ultrasonic oscillation; after fixed-volume and centrifugation, take supernatant filtration
membrane; go through the separation of C18 reverse phase column; then detect at
200nm wave length of UV detector; identify the qualitative as per the chromatographic
retention time; identify the quantification as per the external-standard method.
3 Reagents and Materials
3.1 Acetonitrile (CH3CN). chromatographic pure.
3.2 Phosphoric acid (H3PO4). analytical reagent.
3.3 Potassium dihydrogen phosphate (KH2PO4). analytical reagent.
3.4 0.02mol/L potassium dihydrogen phosphate solution. take 2.722g of
potassium dihydrogen phosphate; add water to dissolve into 1000mL; use
phosphoric acid to adjust the pH value to 3.0.
3.5 Water (H2O). Grade-1 lab water, conductivity (25°C) is 0.01mS/m.
3.6 Calcium pantothenate standard stock solution. weigh 0.1g of calcium
pantothenate standard product (accurate to 0.0001g); place it in a 100mL flask; add
water to dissolve; fix volume to graduation; mix evenly (keep in 4°C refrigerator for
spare use; such solution can be stored for 5d).
3.7 Calcium pantothenate standard use solution. exactly weigh 1.0mL of standard
stock solution (see Clause 3.6); place it in a 100mL flask; add water to dilute to
graduation; mix evenly (keep in 4°C refrigerator for spare use; such solution can be
stored for 5d).
4 Apparatuses and Instruments
4.1 High performance liquid chromatography instrument. attached with UV Detector.
4.2 Ultrasonic cleaner.
4.3 Centrifuge. 4000r/min.
5 Analysis Steps
5.1 Specimen preparation
5.1.1 Solid tablet or capsule specimens. weigh 0.1g~1.0g of post-pulverizing and
uniform specimen (accurate to 0.001g); make the specimen concentration to be within
the standard curve. Place the specimen into a 25mL flask; add 200mL of water; after
conducting ultrasonic extraction for 20min, add water to the graduation; shake
uniformly; after centrifugation, pass the supernatant through the 0.45μm membrane;
the filtrate is to be injected.
5.1.2 Liquid specimen. except that, for carbonated drink, it needs ultrasound to
remove the carbon dioxide before sampling - for other liquid samples, weigh directly
and accurately the appropriate amount of sample-liquid; make the specimen
concentration to be within the standard curve. Place the specimen into the 25mL flask;
use water to dilute to the graduation, and then mix evenly; pass the specimen through
0.45μm membrane, and set aside filtrate.
5.2 Preparation of standard curve
Absorb 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 mL of calcium pantothenate standard use
solution (equivalent to 2.5μg, 5.0μg, 10μg, 20μg, 40μg, and 80μg of calcium
pantothenate), place them into 10mL flasks respectively, dilute with water to the
graduation, and mix evenly. In the given instrument conditions, conduct
......