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GB/T 21995-2025: Determnation of nitroimidazoles in feeds - Liquid chromatography-tandem mass spectrometry
Status: Valid GB/T 21995: Evolution and historical versions
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| GB/T 21995-2025 | English | 229 |
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Determnation of nitroimidazoles in feeds - Liquid chromatography-tandem mass spectrometry
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GB/T 21995-2025
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| GB/T 21995-2008 | English | 189 |
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Determination of nitroimidazoles in feeds -- Liquid chromatography-tandem mass spectrometry method
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GB/T 21995-2008
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PDF similar to GB/T 21995-2025
Basic data | Standard ID | GB/T 21995-2025 (GB/T21995-2025) | | Description (Translated English) | Determnation of nitroimidazoles in feeds - Liquid chromatography-tandem mass spectrometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 11,111 | | Date of Issue | 2025-06-30 | | Date of Implementation | 2026-01-01 | | Older Standard (superseded by this standard) | GB/T 21995-2008 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 21995-2025: Determnation of nitroimidazoles in feeds - Liquid chromatography-tandem mass spectrometry
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National Standard of the People's Republic of China
ICS 65.120CCS B 46
Determination of nitroimidazoles in feed
Liquid chromatography-tandem mass spectrometry
Determination of nitroimidazoles in feeds-
Liquid chromatography⁃tandem mass spectrometry
Released on June 30, 2025
2026-01-01 implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replaces GB/T 21995-2008
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 21995-2008 "Determination of nitroimidazoles in feed by liquid chromatography-tandem mass spectrometry"
Compared with GB/T 21995-2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope of application has been expanded to include mixed feed additives and isopronidazole, and the limits of detection and quantification have been revised (see Chapter 1,.2008).
Chapter 1 of the.2011 edition);
b) The principle, reagents or materials, test procedures, and precision have been changed (see Chapters 4, 5, 8, and 10,.2008 Edition).
Chapters 3, 4, 7, and 9 of the.2012 World Economic Forum).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. China Agricultural University and Henan Agricultural, Animal and Aquatic Products Inspection Technology Research Institute.
The main drafters of this document are. Xia Xi, Wu Ningpeng, Peng Li, Zhao Liang, Meng Lei, Huang Li, Feng Xiaoyou, Li Xiaowei, Jiang Haiyang, and Shen Jianzhong.
The previous versions of this document and the documents it replaces are as follows.
--First published in.2008 as GB/T 21995-2008;
--This is the first revision.
Determination of nitroimidazoles in feed
Liquid chromatography-tandem mass spectrometry
1 Scope
This document describes a method for the determination of five nitroimidazoles in feed by liquid chromatography-tandem mass spectrometry.
This document applies to the use of metronidazole in compound feeds, concentrated feeds, concentrate supplements, additive premixes and mixed feed additives.
Determination of chlorpromazole, dimethicone, ronidazole, isopronidazole and tinidazole.
The detection limit of this document is 5 μg/kg and the quantification limit is 10 μg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Principles
The analytes in the sample were extracted with acetonitrile solution, purified by mixed cation exchange solid phase extraction column, and detected by liquid chromatography-tandem mass spectrometry.
The samples were determined by matrix-matched standard solution calibration and quantified by internal standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. Meet the requirements of Level 1 in GB/T 6682.
5.2 Acetonitrile. chromatographic grade.
5.3 Methanol. chromatographic grade.
5.4 Acetone. chromatographic grade.
5.5 Formic acid. chromatographic grade.
5.6 Acetic acid. chromatographic grade.
5.7 5% formic acid methanol solution. Take 5 mL of formic acid (5.5), dilute to 100 mL with methanol (5.3), and mix well.
5.8 5% ammonia methanol solution. Take 5 mL of ammonia water, dilute it to 100 mL with methanol (5.3), and mix well.
5.9 0.1% Formic Acid Solution. Take 100 μL of formic acid (5.5), dilute to 100 mL with water, and mix thoroughly.
5.10 0.2% acetic acid solution. Take 1 mL of acetic acid (5.6), dilute to 500 mL with water, and mix well.
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