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GB/T 18654.12-2008: Inspection of germplasm for cultured fishes -- Part 12: Method for the Karyotype analysis
Status: Valid GB/T 18654.12: Evolution and historical versions
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| GB/T 18654.12-2008 | English | 229 |
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Inspection of germplasm for cultured fishes -- Part 12: Method for the Karyotype analysis
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GB/T 18654.12-2008
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| GB/T 18654.12-2002 | English | 399 |
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Inspection of germplasm for cultured fishes -- Part 12: Method for the karyotype analysis
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GB/T 18654.12-2002
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Basic data | Standard ID | GB/T 18654.12-2008 (GB/T18654.12-2008) | | Description (Translated English) | Inspection of germplasm for cultured fishes -- Part 12: Method for the Karyotype analysis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 65.150 | | Word Count Estimation | 10,140 | | Date of Issue | 2008-07-31 | | Date of Implementation | 2008-11-01 | | Older Standard (superseded by this standard) | GB/T 18654.12-2002 | | Quoted Standard | GB/T 18654.2 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.12 (Total No.125) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the principles of fish karyotype analysis, instruments and equipment, , slide specimen preparation and karyotype analysis reagents and materials. This section applies to farmed fish karyotype analysis, for natural populations of fish and other aquatic animals may also refer to the implementation. | GB/T 18654.12-2008: Inspection of germplasm for cultured fishes -- Part 12: Method for the Karyotype analysis
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Inspection of germplasm for cultured fishes Part 12. Method for the karyotpe analysis
ICS 65.150
B50
National Standards of People's Republic of China
Replacing GB/T 18654.12-2002
Inspection of germplasm for cultured fishes
Part 12. karyotyping
Posted 2008-07-31
2008-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 18654 "Inspection of germplasm for cultured fishes" is divided into the following sections.
--- Part 1. Inspection rules;
--- Part 2. Sampling methods;
--- Part 3. Determination of traits;
--- Part 4. Determination of age and growth;
--- Part 5. Diet analysis;
--- Part 6. Determination of reproductive performance;
--- Part 7. Ecological Characteristics;
--- Part 8. Determination of the oxygen consumption rate and the critical point of suffocation;
--- Part 9. Includes meat for determination;
--- Part 10. Determination of muscle nutrients;
--- Part 11. Determination of the major muscle amino acid contents;
--- Part 12. karyotype analysis;
--- Part 13. isoenzyme electrophoresis analysis;
--- Part 14. Determination of DNA content;
--- Part 15. RAPD analysis;
This section GB/T Part of 1,218,654.
This Part replaces GB/T 18654.12-2002 "Inspection of germplasm for cultured fishes - Part 12. karyotype analysis."
This section compared with the GB/T 18654.12-2002 main changes are as follows.
--- Increasing the digital camera and other advanced technologies;
--- 7.2.9 of the low permeability time adjusted amended; concentration of 5.4 sodium bicarbonate (NaHCO3) has done a supplement;
Amendments and supplements to be part of the operating method described 3.6,7.2.4,7.2.12,7.3.2 and 8.3; for in Chapter 6 of the instrument set
Be prepared to supplement and improve;
--- On the part of the experiment described in Chapter 3 to Chapter 8 of the modification accurate description of the part of the text to be corrected;
--- Appendix A. 3.4 and A. 3.5 part of the text description be amended to increase the B. 2.1 Digital photomicrography content.
Appendix A of this section and Appendix B is an informative annex.
This section proposed by the People's Republic of China Ministry of Agriculture.
This part of the National Standardization Technical Committee of aquatic freshwater aquaculture division Technical Committee.
This section drafted by. Chinese Academy of Fishery Sciences, Yangtze River Fisheries Research Institute.
The main drafters of this section. Fangyao Lin, Zhou Ruiqiong, Zou Shiping.
This part of the standard replaces the previous editions are.
--- GB/T 18654.12-2002.
Inspection of germplasm for cultured fishes
Part 12. karyotyping
1 Scope
This section GB/T 18654 specifies the principles of fish karyotype analysis, reagents and materials, instruments and equipment, slide specimen
Preparation and karyotype analysis.
This section applies to farmed fish karyotype analysis, for natural populations of fish and other aquatic animals also implementation.
2 Normative references
The following documents contain provisions which, through reference GB/T 18654 in this section constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB/T 18654.2 Inspection of germplasm for cultured fishes - Part 2. Sampling Method
3 Terms and Definitions
The following terms and definitions apply GB/T 18654 in this section.
3.1
By aseptic operation, with fish kidney tissue cells, or blood cells, cultured in vitro during cell division added stimulus, stimulating shower
Pakistan poured into the cell divided. Then, the appropriate concentration of colchicine, cell division in metaphase is repression, and access to fish
Class of metaphase chromosomes.
3.2
To fish by injecting cell division stimulus, stimulate lymphocytes poured into the split state. Remove the head kidney tissue in physiological saline
In its full pieces, then add the appropriate concentration of colchicine.
3.3
The fish was immersed in an appropriate concentration of colchicine solution to split vigorous gill epithelial cells are repressor in metaphase, and eligible for
Fish have metaphase chromosome.
3.4
Selection and development of normal blastocysts or early gastrula embryos, after which cells were dispersed by pipetting, adding the appropriate concentration of colchicine, the fine
Inhibitory cells in metaphase, get fish metaphase chromosome.
3.5
The collected cells after hypotonic, fixed, drop sheet, then diagonal standing, let it dry naturally.
3.6
The collected cells after hypotonic, fixed, drop sheet, then immediately slide into alcohol lamp flame evenly baked (in the slide just left
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