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GB/T 18090-2023: Diagnostic techniques for porcine reproductive and respiratory syndrome
Status: Valid GB/T 18090: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18090-2023 | English | 519 |
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Diagnostic techniques for porcine reproductive and respiratory syndrome
| Valid |
GB/T 18090-2023
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| GB/T 18090-2008 | English | 799 |
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Diagnostic methods of porcine reproductive and respiratory syndrome
| Obsolete |
GB/T 18090-2008
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| GB/T 18090-2000 | English | 599 |
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Diagnostic methods of porcine reproductive and respiratory syndrome
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GB/T 18090-2000
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PDF similar to GB/T 18090-2023
Basic data | Standard ID | GB/T 18090-2023 (GB/T18090-2023) | | Description (Translated English) | Diagnostic techniques for porcine reproductive and respiratory syndrome | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 26,280 | | Date of Issue | 2023-03-17 | | Date of Implementation | 2023-10-01 | | Older Standard (superseded by this standard) | GB/T 18090-2008 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18090-2023: Diagnostic techniques for porcine reproductive and respiratory syndrome---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standards of People's Republic of China
Replacing GB/T 18090-2008
Diagnostic methods of porcine reproductive and respiratory syndrome
Released on 2023-03-17
2023-10-01 implementation
State Administration for Market Regulation
Released by the National Standardization Management Committee
table of contents
Preface III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Biosecurity measures 2
6 Clinical Diagnosis 2
6.1 Susceptible animals 2
6.2 Clinical symptoms 2
6.3 Pathological changes 2
6.4 Result Judgment 2
7 Collection and storage of laboratory test samples 3
7.1 Sampling equipment 3
7.2 Sample collection 3
7.3 Sample storage 3
8 Virus isolation and identification 3
8.1 Main equipment, reagents and cells 3
8.2 Sample preparation 4
8.3 Test method 4
8.4 Result Judgment 5
9 Reverse transcription-polymerase chain reaction (RT-PCR) 5
9.1 Main Equipment 5
9.2 Main reagents 5
9.3 Sample preparation 6
9.4 Test method 6
9.5 Result Judgment 8
10 Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) 8
10.1 Main Equipment 8
10.2 Main Reagents 8
10.3 Sample preparation 8
10.4 Test method 8
10.5 Result Judgment 9
11 Immunoperoxidase Monolayer Assay (IPMA) 9
11.1 Main Equipment 9
11.2 Main reagents 10
11.3 Test method 10
11.4 Result Judgment 10
12 Indirect Immunofluorescence Assay (IFA) 10
12.1 Main Equipment 10
12.2 Main reagents 10
12.3 Test method 10
12.4 Result Judgment 11
13 Indirect enzyme-linked immunosorbent assay (indirect ELISA) 11
13.1 Main Equipment 11
13.2 Main reagents 11
13.3 Test method 11
13.4 Result judgment 12
14 comprehensive judgment 12
Appendix A (Normative) Preparation, Identification and Storage of Porcine Alveolar Macrophages (PAMs) 14
Appendix B (Normative) Preparation of Reagents 16
Appendix C (Informative) RT-PCR, Quantitative RT-PCR Primer Probes and Specific Amplified Fragment Sequences 18
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents"
drafting.
This document replaces GB/T 18090-2008 "Diagnostic Methods for Porcine Reproductive and Respiratory Syndrome". Compared with GB/T 18090-2008, except
Apart from structural adjustments and editorial changes, the main technical changes are as follows.
---Changed the clinical diagnosis (see Chapter 6, Chapter 4 of the.2008 edition);
--- Increased real-time reverse transcription polymerase chain reaction (see Chapter 10).
Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This document is drafted by. Dalian Customs of the People's Republic of China, China Agricultural University, and Zhengzhou Customs of the People's Republic of China.
The main drafters of this document. Hu Chuanwei, Yang Hanchun, Zhao Huijun, Zhou Lei, Zhang Xue, Liu Zhao, Miao Li, Jia Yun, Li Ye.
The release status of previous versions of this document and the documents it replaces are as follows.
---It was first published as GB/T 18090-2000 in.2000 and revised for the first time in.2008;
--- This is the second revision.
Diagnostic methods of porcine reproductive and respiratory syndrome
1 Scope
This document describes methods for the clinical and laboratory diagnosis of porcine reproductive and respiratory syndrome (PRRS).
This document applies to the diagnosis and monitoring of porcine reproductive and respiratory syndrome. Clinical diagnostic methods are suitable for clinically suspected cases of PRRS
Diagnosis, virus isolation and identification, reverse transcription-polymerase chain reaction (RT-PCR) and real-time reverse transcription-polymerase chain reaction (Real-timeRT-PCR)
PCR) is suitable for the detection of porcine reproductive and respiratory syndrome virus (PRRSV), immunoperoxidase monolayer assay (IPMA), indirect immunoassay
Fluorescence assay (IFA) and indirect enzyme-linked immunosorbent assay (ELISA) are suitable for the detection of PRRSV antibodies and the monitoring of infection status.
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General Requirements for Laboratory Biosafety
NY/T 541 Technical specification for collection, preservation and transportation of veterinary diagnostic samples
3 Terms and Definitions
This document does not have terms and definitions that need to be defined.
4 Abbreviations
The following abbreviations apply to this document.
AEC. Aminoethylcarbazole (3-amino-9-ethyl-carbazole)
bp. base pair (basepair)
CPE. cytopathic effect (cytopathic effect)
EB. ethidium bromide
OD. optical density (opticaldensity)
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