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GB/T 15805.3-2018 English PDF

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GB/T 15805.3-2018: Code of diagnosis for viral haemorrhagic septicaemia (VHS)
Status: Valid

GB/T 15805.3: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 15805.3-2018English359 Add to Cart 4 days [Need to translate] Code of diagnosis for viral haemorrhagic septicaemia (VHS) Valid GB/T 15805.3-2018
GB/T 15805.3-2008English279 Add to Cart 3 days [Need to translate] The quarantine methods of fish -- Part 3: Viral hemorrhagic septicemia virus (VHSV) Obsolete GB/T 15805.3-2008

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Basic data

Standard ID GB/T 15805.3-2018 (GB/T15805.3-2018)
Description (Translated English) Code of diagnosis for viral haemorrhagic septicaemia (VHS)
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 65.020.30
Word Count Estimation 18,166
Date of Issue 2018-07-13
Date of Implementation 2019-02-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 15805.3-2018: Code of diagnosis for viral haemorrhagic septicaemia (VHS)

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Code of diagnosis for viral haemorrhagic septicaemia (VHS) ICS 65.020.30 B41 National Standards of People's Republic of China Replace GB/T 15805.3-2008 Diagnostic protocol for viral hemorrhagic sepsis Published on.2018-07-13 Implementation of.2019-02-01 State Market Supervisory Administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 15805.3-2008 "Fish quarantine methods - Part 3. Viral hemorrhagic sepsis virus (VHSV). Compared with GB/T 15805.3-2008, this standard is subject to the following technical changes as follows. --- Increased sampling; --- Increased clinical symptoms; --- Increased indirect immunofluorescent antibody test (IFAT) for detection of virality a method of hemorrhagic sepsis virus antigen; --- Added enzyme-linked immunosorbent assay (ELISA) to detect viral hemorrhagic a method of septic virus antigen; --- Added RT-PCR and fluorescent RT-PCR detection methods; --- Added comprehensive judgment; --- Added a normative appendix to VHS. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. National Aquatic Technology Extension Station, Shandong Entry-Exit Inspection and Quarantine Bureau, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Beijing Entry Inspection and Quarantine Bureau. The main drafters of this standard. Yu Weizhong, Sun Tao, Yin Weili, Jia Peng, Lu Yonghui, Gu Qiang, Zhang Lifeng, Yue Zhiqin, Wang Jinjin, Jiang Yulin. The previous versions of the standards replaced by this standard are. ---GB/T 15805.1-1995; ---GB/T 15805.3-2008. Diagnostic protocol for viral hemorrhagic sepsis

1 Scope

This standard specifies the clinical symptoms, histopathological features, virus isolation, neutralization test, indirect fluorescent antibody of viral hemorrhagic septicaemia. Tests, enzyme-linked immunosorbent assays, reverse transcriptase polymerase chain reaction, and real-time fluorescent polymerase chain reaction were used to identify methods. This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of fish viral hemorrhagic sepsis.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 18088 entry and exit animal quarantine sampling SC/T 7016.1 Fish Cell Line Part 1. Fat Head Muscle Cell Line (FHM) SC/T 7016.5 Fish Cell Lines Part 5. Epithelial Tumor Cell Line (EPC) SC/T 7016.9 Fish Cell Lines Part 9. Blue Sunfish Cell Line (BF-2) SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. BF-2. Blue carp sunfish cell line (bluegilfrycelline) Bp. basepair CPE. cytopathic effect CTAB. cetyltrimethylammonium bromide Ct. cycle threshold (cyclethreshold) cDNA. Complementary deoxyribonucleic acid (complementarydeoxyribonucleicacid) DNA. deoxyribonucleic acid DEPC. diethylpyrocarbonate dNTP. deoxy-ribonucleoside triphosphate EDTA. ethylenediaminetetraacetic acid EPC. epithelioma cell line (epitheliomapapulosumcyprinicelline) FBS. fetal bovine serum (fetalbovineserum) FHM. fat head muscle cell line (fatheadmonnowcelline) FITC. fluorescein isothiocyanate HEPES. 4-hydroxyethylpiperazineethanesulfonic acid [4-(2-hydroxyethy1)-1-piperazineethanesulfonic acid] OPD. o-phenylenediamine PBS. phosphate buffer solution PBST. phosphate buffer solution (phosphatebuffersolution-tween-20)

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