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GB/T 15805.4-2018 English PDF

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GB/T 15805.4-2018: Code of diagnosis for channel catfish virus diseases
Status: Valid

GB/T 15805.4: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB/T 15805.4-2018English279 Add to Cart 3 days [Need to translate] Code of diagnosis for channel catfish virus diseases Valid GB/T 15805.4-2018
GB/T 15805.4-2008English359 Add to Cart 3 days [Need to translate] The quarantine methods of fish -- Part 4: Channel catfish virus (CCV) Obsolete GB/T 15805.4-2008

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Basic data

Standard ID GB/T 15805.4-2018 (GB/T15805.4-2018)
Description (Translated English) Code of diagnosis for channel catfish virus diseases
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 65.020.30
Word Count Estimation 14,168
Date of Issue 2018-07-13
Date of Implementation 2019-02-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 15805.4-2018: Code of diagnosis for channel catfish virus diseases

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of diagnosis for channel catfish virus diseases ICS 65.020.30 B41 National Standards of People's Republic of China Replace GB/T 15805.4-2008 Diagnostic protocol for channel catfish virus Published on.2018-07-13 Implementation of.2019-02-01 State Market Supervisory Administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 15805.4-2008 "Fish quarantine method Part 4. Channel catfish virus (CCV)". This standard Compared with GB/T 15805.4-2008, except for editorial changes, the main technical changes are as follows. --- Increased clinical symptoms of channel catfish virus disease; --- Added a method for detecting channel catfish virus antigen by enzyme-linked immunosorbent assay; --- Changed to phenol chloroform method for extracting viral nucleic acid; --- PCR detection increased internal control DNA, length 186bp; --- Added informative Appendix C "Channel Catfish Virus Disease (CCVD)". Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. National Aquatic Technology Extension Station, China Academy of Inspection and Quarantine, Beijing Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Lu Yonghui, Zhang Wei, Wang Na, Gu Qiang, Yu Weizhong, Jing Hongli, Zhang Lifeng, Xu Wei, Zhou Yi. The previous versions of the standards replaced by this standard are. ---GB/T 15805.1-1995; ---GB/T 15805.4-2008. Diagnostic protocol for channel catfish virus

1 Scope

This standard specifies the clinical symptoms, histopathological features, virus isolation, neutralization test, enzyme-linked immunosorbent assay for channel catfish virus Test and polymerase chain reaction detection methods. This standard applies to epidemiological investigation, diagnosis, quarantine and monitoring of channel catfish virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 18088 entry and exit animal quarantine sampling SC/T 7016.8 Fish Cell Lines Part 8. Channel catfish ovary cell line (CCO) SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. Bp. basepair CCO. channel catfish ovary cells (channelcatfishovarycelline) CCV. Channel catfish virus (channelcatfishvirus) CCVD. channel catfish virus disease (channelcatfishvirusdisease) CPE. cytopathic effect dATP. deoxyadenosine triphosphate (deoxyadenosinetriphosphate) dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate) dGTP. deoxyguannosine triphosphate (deoxyaguanosinetriphosphate) DNA. deoxyribonucleic acid dNTP. deoxy-ribonucleoside triphosphate dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate) EB. ethidium bromide (ethidiumbromide) EDTA. ethylenediaminetetraacetic acid ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay) FBS. fetal bovine serum (fetalbovineserum) IgG. immunoglobulin G (immunoglobulinG) OD. optical density (optical density) OPD. o-phenylenediamine PBST. phosphate buffer solution (phosphatebuffersolution-tween-20) PCR. polymerase chain reaction (polymerase chain reaction)

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