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SN/T 4714-2016: Technical specification for DNA barcodes database
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SN/T 4714-2016: Technical specification for DNA barcodes database

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Technical specification for DNA barcodes database People's Republic of China Exit Inspection and Quarantine standards DNA Bar Code Database Technical Specifications 2016-12-12 released 2017-07-01 implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released Exit Inspection and Quarantine of People's Republic of China Industry Standard DNA Bar Code Database Technical Specifications China Standard Press First edition of November.2017

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard drafting unit. China Inspection and Quarantine Science Institute, the State Administration of Quality Supervision, Inspection and Quarantine Information Center, People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Bureau, Institute of Microbiology Chinese Academy of Sciences. The main drafters of this standard. Jiang Hongshan, Zhang Yanping, Lei Rong, Yue Qiao Yun, Jun Ma, Chen Ke, Liu force. DNA Bar Code Database Technical Specifications

1 Scope

This standard specifies the DNA bar code classification code, entity coding, attribute data structure, database construction, operation and maintenance techniques Technical specifications. This standard applies to guide the quarantine pest DNA bar code database construction and data exchange.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 2260-2007 Administrative divisions of the People's Republic of China code SN/T 4621 Medical Media Biological Specs Code

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 DNA barcode DNAbarcode The organism can represent a standard, well-mutated, and easily amplified DNA signature of the species. 3.2 Candidate genes candidategenes Use DNA barcoding for species selection for selection of genes. The ideal candidate gene has the following characteristics. Interspecific genetic distance Significantly greater than the intraspecific genetic distance, and overlapping area is small or none. Commonly used candidate genes are mitochondrial cytochrome C oxidase I subunit (COI) Gene, rDNAITS sequence, plastid trnH-psbA sequence, chloroplast rbcL sequence and the like. 3.3 Polymerase chain reaction polymerase chain reaction PCR A molecular biology technique used to amplify specific DNA fragments. In this reaction, oligos complementary to the DNA sequence of interest are used Nucleotides serve as primers for multiple rounds of DNA synthesis. These include DNA denaturation, primer annealing and polymerase-catalyzed synthesis. 3.4 Sequencing experiment information sequencingruninformation Determination of DNA barcode related PCR amplification primers, sequencing primers, experimental procedures, sequencing time, sequencing machinery, sequencing the original peak Map and other information. 3.5 Voucher specimens voucherspecimen Complete collection, identification information (collectors, dates, locations, habitats, appraisers, species name, etc.), for species review, citation, traceability and Permanently preserved specimens (or preserved specimens). ...