SN/T 4708-2016 English PDF

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SN/T 4708-2016: Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4708-2016	229	Add to Cart	3 days	Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports	Valid

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Basic data

Standard ID: SN/T 4708-2016 (SN/T4708-2016)
Description (Translated English): Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Word Count Estimation: 10,174
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4708-2016: Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method of vibrio cholerae and vibrio mimicus by duplex real-time PCR at frontier ports People's Republic of China Exit Inspection and Quarantine standards Vibrio cholerae and Vibrio mimicus double fluorescence at the border crossing PCR detection method 2016-12-12 released 2017-07-01 implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard drafting unit. China Inspection and Quarantine Science Institute, People's Republic of China Jiangsu Exit Inspection and Quarantine. The main drafters of this standard. Han Hui, Yang Yu, Wu Hailei, Tan Ke, Song Yan Beijing, Li Shan, Xu Baoliang. Vibrio cholerae and Vibrio mimicus double fluorescence at the border crossing PCR detection method

1 Scope

This standard specifies the border crossing Vibrio cholerae and mimic Vibrio testing methods, including the detection of objects, sample collection and processing, the operator Law and Results Report. This standard applies to entry and exit personnel at border crossings, environmental water carrying Vibrio cholerae (including O1 group, O139 group and non-O1 group / O139 Vibrio cholerae) and mimicry Vibrio molecular biology tests.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article For all undated references, the latest edition (including all amendments) applies to this document. GB/T 19489-2008 Laboratory biosafety general requirements General Principles of Biosafety in Microbiology and Biomedical Laboratories Regulations on the Transport and Administration of Highly Pathogenic Microbial (Poisonous) Bacteria or Species That Can Infect Human Beings (Ministry of Health Decree No. 45)

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Vibrio Cholerae Vibrio, Gram stain negative. Patients and carriers are the main source of cholera, contaminated by excrement or excrement from patients and carriers Water or food can cause cholera outbreak. Cholera has the heat-resistant O antigen and the heat-labile H antigen. According to different O antigen, Huo Vibrio cholerae can be divided into more than.200 serotypes, of which only O1 and O139 Vibrio cholerae strains can cause cholera epidemic. 3.2 Vibrio mimicus Vibrio, Gram stain negative. Widely found in nature water, sea water and seafood. Is a human and aquatic animal disease Pathogens, which infect humans through water and aquatic products, have become pathogenic to sporadic and epidemic diarrhea and food poisoning in some areas one. Vibrio mimicry biochemical characteristics and Vibrio cholerae are very similar, mainly caused by human acute diarrhea.

4 Abbreviations

The following abbreviations apply to this document. PCR. Polymerase chain reaction DNA. DNA DEPC. Dicobal FAM.6-carboxy-fluorescein, a fluorescent reporter group HEX. Hexachloro-6-methylfluorescein, a fluorescent reporter ......

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