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Technical regulation of farm animals somatic cell bank detection
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Basic data
| Standard ID | GB/T 24862-2010 (GB/T24862-2010) |
| Description (Translated English) | Technical regulation of farm animals somatic cell bank detection |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B40 |
| Classification of International Standard | 65.020.30 |
| Word Count Estimation | 10,178 |
| Date of Issue | 2010-06-30 |
| Date of Implementation | 2011-01-01 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | National Standard Approval Announcement 2010 No.2 (Total No.157) |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the method for detection of animal somatic cell library. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry in vitro cell identification. |
GB/T 24862-2010: Technical regulation of farm animals somatic cell bank detection
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Technical regulation of farm animals somatic cell bank detection
ICS 65.020.30
B40
National Standards of People's Republic of China
Animal somatic cell library Technical specification for testing
Issued on. 2010-06-30
2011-01-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is an informative annex.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized.
This standard was drafted. Beijing Animal Husbandry and Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Mayue Hui, Wei Guan Jun, Lixiang Chen, in too Yong, Li Han, Xiao-hong, Liu Tao.
Animal somatic cell library Technical specification for testing
1 Scope
This standard specifies the method for the detection of livestock somatic cell library.
This standard applies to pigs, cattle, sheep, horses, donkeys, chickens, ducks, geese and other poultry cultured cells were identified.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
The total percentage of cells in living cells.
3.2
In the generation of cell survival period, based on the incubation period, exponential growth, stagnation and decline of the dynamic changes in cell number, cell culture
Time as the abscissa, cell density Growth Rules ordinate cell plotted curve.
3.3
Somatic metaphase chromosomes relatively constant set of features according to their number, length, centromere position, with the body, the primary and secondary constriction constriction, etc.
Line up the image.
3.4
The same structure and function of a different type catalytic enzyme.
3.5
The proportion of its cell culture surface.
4 Workspace conditions
Workspace generally from the preparation room, buffer room, sterile room, cell preservation chamber.
The inter-buffer area should not be less than 3m2, and a locker and ultraviolet light, ultraviolet light intensity is not less than 1.5W/m3. UV lamps
Pipe from the ground should not exceed 2.5m, each exposure time was 20min ~ 30min. Minimum standards Aseptic sterile room level should reach ten thousand.
5 major instruments and equipment
Electrophoresis and electrophoresis tank, clean table, refrigerator, oven blast, high-pressure steam sterilizer, liquid nitrogen biological containers, centrifuges, electronic day
Flat, incubator, CO2 incubator, ultra-pure water equipment, automatic gel imaging analysis system, and microscopes.
...
